Supplementation of lake extender with cysteamine preserves quality parameters and fertility potential of post-thawed rooster sperm during cryopreservation process
Abstract Background In the cryopreservation process, rooster spermatozoa are vastly sensitive to reactive oxygen species (ROS). This study aimed to investigate the effects of Lake extender supplemented via Cysteamine (CYS) on the quality and fertility characteristics of rooster semen during the cryo...
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BMC
2025-01-01
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| Series: | BMC Veterinary Research |
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| Online Access: | https://doi.org/10.1186/s12917-024-04468-z |
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| author | Reza Masoudi Saeid Esmaeilkhanian Maryam Hatami Mohsen Sharafi Ali Hatefi Fatemeh Zarei Reihaneh Nateghi Mohammad Reza Tohidkia |
| author_facet | Reza Masoudi Saeid Esmaeilkhanian Maryam Hatami Mohsen Sharafi Ali Hatefi Fatemeh Zarei Reihaneh Nateghi Mohammad Reza Tohidkia |
| author_sort | Reza Masoudi |
| collection | DOAJ |
| description | Abstract Background In the cryopreservation process, rooster spermatozoa are vastly sensitive to reactive oxygen species (ROS). This study aimed to investigate the effects of Lake extender supplemented via Cysteamine (CYS) on the quality and fertility characteristics of rooster semen during the cryopreservation process. Methods Semen samples were collected from 10 proved Ross-308 roosters, diluted and cryopreserved in the Lake extender which contained 0, 1, 2, 4, and 8 mM of CYS (C-0, C-1, C-2, C-4, and C-8, respectively). Motility parameters, membrane integrity, abnormal morphology, mitochondrial activity, acrosome integrity, viability, apoptosis status, lipid peroxidation, DNA fragmentation, ROS concentration, as well as fertility potential were evaluated after thawing. Results total motility and progressive motility were higher (P ≤ 0.05) in C-1 and C-2 compared to the other groups. The C-1 showed higher (P ≤ 0.05) membrane integrity, mitochondrial activity, acrosome integrity, viability, and lower (P ≤ 0.05) late apoptosis, lipid peroxidation, DNA fragmentation, and ROS concentration compared to the other groups. During fertility evaluation, C-1 presented a higher fertility rate than the control group. In cases of velocity parameters, abnormal morphology, early apoptosis, necrosis, and hatching rate, no significant difference (P > 0.05) was found. Conclusion using the optimal concentration of CYS (1 mM) in the Lake extender is useful for protecting rooster sperm during the cryopreservation process and it could be used for reproductive programs. |
| format | Article |
| id | doaj-art-e0d784a223f34ba28f51c92685e33b99 |
| institution | Kabale University |
| issn | 1746-6148 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Veterinary Research |
| spelling | doaj-art-e0d784a223f34ba28f51c92685e33b992025-01-19T12:27:16ZengBMCBMC Veterinary Research1746-61482025-01-012111910.1186/s12917-024-04468-zSupplementation of lake extender with cysteamine preserves quality parameters and fertility potential of post-thawed rooster sperm during cryopreservation processReza Masoudi0Saeid Esmaeilkhanian1Maryam Hatami2Mohsen Sharafi3Ali Hatefi4Fatemeh Zarei5Reihaneh Nateghi6Mohammad Reza Tohidkia7Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO)Animal Science Research Institute of Iran (ASRI), Agricultural Research Education and Extension Organization (AREEO)Department of Animal Science, Faculty of Agriculture, University of TabrizSemex AllianceDepatment of Animal Science, University of TehranDepartment of Animal Science, Faculty of Agriculture, University of TabrizDepartment of Animal and Poultry Physiology, Faculty of Animal Science, Gorgan University of Agricultural Science and Natural ResourcesResearch Center for Pharmaceutical Nanotechnology, Tabriz University of Medical SciencesAbstract Background In the cryopreservation process, rooster spermatozoa are vastly sensitive to reactive oxygen species (ROS). This study aimed to investigate the effects of Lake extender supplemented via Cysteamine (CYS) on the quality and fertility characteristics of rooster semen during the cryopreservation process. Methods Semen samples were collected from 10 proved Ross-308 roosters, diluted and cryopreserved in the Lake extender which contained 0, 1, 2, 4, and 8 mM of CYS (C-0, C-1, C-2, C-4, and C-8, respectively). Motility parameters, membrane integrity, abnormal morphology, mitochondrial activity, acrosome integrity, viability, apoptosis status, lipid peroxidation, DNA fragmentation, ROS concentration, as well as fertility potential were evaluated after thawing. Results total motility and progressive motility were higher (P ≤ 0.05) in C-1 and C-2 compared to the other groups. The C-1 showed higher (P ≤ 0.05) membrane integrity, mitochondrial activity, acrosome integrity, viability, and lower (P ≤ 0.05) late apoptosis, lipid peroxidation, DNA fragmentation, and ROS concentration compared to the other groups. During fertility evaluation, C-1 presented a higher fertility rate than the control group. In cases of velocity parameters, abnormal morphology, early apoptosis, necrosis, and hatching rate, no significant difference (P > 0.05) was found. Conclusion using the optimal concentration of CYS (1 mM) in the Lake extender is useful for protecting rooster sperm during the cryopreservation process and it could be used for reproductive programs.https://doi.org/10.1186/s12917-024-04468-zApoptosis statusCryopreservationCysteamineFertilityRooster |
| spellingShingle | Reza Masoudi Saeid Esmaeilkhanian Maryam Hatami Mohsen Sharafi Ali Hatefi Fatemeh Zarei Reihaneh Nateghi Mohammad Reza Tohidkia Supplementation of lake extender with cysteamine preserves quality parameters and fertility potential of post-thawed rooster sperm during cryopreservation process BMC Veterinary Research Apoptosis status Cryopreservation Cysteamine Fertility Rooster |
| title | Supplementation of lake extender with cysteamine preserves quality parameters and fertility potential of post-thawed rooster sperm during cryopreservation process |
| title_full | Supplementation of lake extender with cysteamine preserves quality parameters and fertility potential of post-thawed rooster sperm during cryopreservation process |
| title_fullStr | Supplementation of lake extender with cysteamine preserves quality parameters and fertility potential of post-thawed rooster sperm during cryopreservation process |
| title_full_unstemmed | Supplementation of lake extender with cysteamine preserves quality parameters and fertility potential of post-thawed rooster sperm during cryopreservation process |
| title_short | Supplementation of lake extender with cysteamine preserves quality parameters and fertility potential of post-thawed rooster sperm during cryopreservation process |
| title_sort | supplementation of lake extender with cysteamine preserves quality parameters and fertility potential of post thawed rooster sperm during cryopreservation process |
| topic | Apoptosis status Cryopreservation Cysteamine Fertility Rooster |
| url | https://doi.org/10.1186/s12917-024-04468-z |
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