PCR for M. tuberculosis in tissue samples
Background: The presence of granuloma, visualized in histopathology for diagnosing tuberculosis in tissue samples, is not a specific finding. Moreover, histopathological examination of tissue sections needs one to two weeks for final reporting. A rapid and sensitive method is therefore needed for de...
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| Format: | Article |
| Language: | English |
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The Journal of Infection in Developing Countries
2009-03-01
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| Series: | Journal of Infection in Developing Countries |
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| Online Access: | https://jidc.org/index.php/journal/article/view/53 |
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| author | Kiran Chawla Soham Gupta Chiranjay Mukhopadhyay P. Sugandhi Rao Sudha S. Bhat |
| author_facet | Kiran Chawla Soham Gupta Chiranjay Mukhopadhyay P. Sugandhi Rao Sudha S. Bhat |
| author_sort | Kiran Chawla |
| collection | DOAJ |
| description | Background: The presence of granuloma, visualized in histopathology for diagnosing tuberculosis in tissue samples, is not a specific finding. Moreover, histopathological examination of tissue sections needs one to two weeks for final reporting. A rapid and sensitive method is therefore needed for detection of Mycobacterium tuberculosis in these paucibacillary tissue samples.
Methodology: A PCR-assay specific for IS6110 was evaluated for 104 different tissue samples in comparison to histopathology that was considered gold standard.
Results: PCR showed 74.1% sensitivity and 96.1% specificity. False positive and false negative results were observed in three (2.88%) and seven (6.73%) samples, respectively. Positive agreement between histopathology and PCR was observed as 0.737, indicating substantial good agreement between two tests.
Conclusions: PCR can be used for early diagnosis of tuberculosis in tissue samples that can help to initiate timely anti-tubercular treatment and prevent progression to irreversible changes. |
| format | Article |
| id | doaj-art-e0d57861004442bc96a455ccfd4e2fbf |
| institution | DOAJ |
| issn | 1972-2680 |
| language | English |
| publishDate | 2009-03-01 |
| publisher | The Journal of Infection in Developing Countries |
| record_format | Article |
| series | Journal of Infection in Developing Countries |
| spelling | doaj-art-e0d57861004442bc96a455ccfd4e2fbf2025-08-20T02:57:53ZengThe Journal of Infection in Developing CountriesJournal of Infection in Developing Countries1972-26802009-03-0130210.3855/jidc.53PCR for M. tuberculosis in tissue samplesKiran Chawla0Soham Gupta1Chiranjay Mukhopadhyay2P. Sugandhi Rao3Sudha S. Bhat4Department of Microbiology and Pathology, Kasturba Medical College, Manipal. KarnatakaDepartment of Microbiology and Pathology, Kasturba Medical College, Manipal. KarnatakaDepartment of Microbiology and Pathology, Kasturba Medical College, Manipal. KarnatakaDepartment of Microbiology and Pathology, Kasturba Medical College, Manipal. KarnatakaDepartment of Microbiology and Pathology, Kasturba Medical College, Manipal. KarnatakaBackground: The presence of granuloma, visualized in histopathology for diagnosing tuberculosis in tissue samples, is not a specific finding. Moreover, histopathological examination of tissue sections needs one to two weeks for final reporting. A rapid and sensitive method is therefore needed for detection of Mycobacterium tuberculosis in these paucibacillary tissue samples. Methodology: A PCR-assay specific for IS6110 was evaluated for 104 different tissue samples in comparison to histopathology that was considered gold standard. Results: PCR showed 74.1% sensitivity and 96.1% specificity. False positive and false negative results were observed in three (2.88%) and seven (6.73%) samples, respectively. Positive agreement between histopathology and PCR was observed as 0.737, indicating substantial good agreement between two tests. Conclusions: PCR can be used for early diagnosis of tuberculosis in tissue samples that can help to initiate timely anti-tubercular treatment and prevent progression to irreversible changes.https://jidc.org/index.php/journal/article/view/53tissue samplestuberculosisPCRhistopathology |
| spellingShingle | Kiran Chawla Soham Gupta Chiranjay Mukhopadhyay P. Sugandhi Rao Sudha S. Bhat PCR for M. tuberculosis in tissue samples Journal of Infection in Developing Countries tissue samples tuberculosis PCR histopathology |
| title | PCR for M. tuberculosis in tissue samples |
| title_full | PCR for M. tuberculosis in tissue samples |
| title_fullStr | PCR for M. tuberculosis in tissue samples |
| title_full_unstemmed | PCR for M. tuberculosis in tissue samples |
| title_short | PCR for M. tuberculosis in tissue samples |
| title_sort | pcr for m tuberculosis in tissue samples |
| topic | tissue samples tuberculosis PCR histopathology |
| url | https://jidc.org/index.php/journal/article/view/53 |
| work_keys_str_mv | AT kiranchawla pcrformtuberculosisintissuesamples AT sohamgupta pcrformtuberculosisintissuesamples AT chiranjaymukhopadhyay pcrformtuberculosisintissuesamples AT psugandhirao pcrformtuberculosisintissuesamples AT sudhasbhat pcrformtuberculosisintissuesamples |