The Effect of High Intensity Focused Ultrasound Keratoplasty on Rabbit Anterior Segment
Purpose. To evaluate the safety of high-intensity focused ultrasound keratoplasty as a treatment for presbyopia by examining its effect on the rabbit anterior segment. Methods. The right corneas of 36 New Zealand rabbits were treated with HIFU keratoplasty. The animals were sacrificed at 1, 7, 15, 3...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2017/6067890 |
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| author | Menglei Wang Meixuan Li Pisong Yan Qiang Luo Yu Zhang Zhiyu Du |
| author_facet | Menglei Wang Meixuan Li Pisong Yan Qiang Luo Yu Zhang Zhiyu Du |
| author_sort | Menglei Wang |
| collection | DOAJ |
| description | Purpose. To evaluate the safety of high-intensity focused ultrasound keratoplasty as a treatment for presbyopia by examining its effect on the rabbit anterior segment. Methods. The right corneas of 36 New Zealand rabbits were treated with HIFU keratoplasty. The animals were sacrificed at 1, 7, 15, 30, 60, and 90 days after operation. Collagen type I, MMP-2, and MMP-9 were evaluated using immunohistochemistry. For the detection of apoptosis, the TUNEL method was applied. The SOD and MDA levels were analyzed with assay kits. Results. Collagen type I, MMP-2, and MMP-9 levels were altered after the operation but returned to normal within 90 days. The apoptotic index (AI) of the corneal cells decreased from 1 to 30 days gradually. No apoptosis was observed in the epithelial cells of the lens, and the SOD and MDA levels were normal at any time point. Conclusion. After HIFU keratoplasty, the histomorphology of the cornea changed, the corneal collagen type I levels decreased, the corneal MMP-2 and MMP-9 levels increased, and the corneal cells underwent apoptosis for a period of time. Ninety days after the operation, the levels returned to normal, and the lenses were not affected. Thus, HIFU presents good biological safety for eyes. |
| format | Article |
| id | doaj-art-e0c29cbefa4b4d388cf143dc82687116 |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-e0c29cbefa4b4d388cf143dc826871162025-02-03T06:44:19ZengWileyJournal of Ophthalmology2090-004X2090-00582017-01-01201710.1155/2017/60678906067890The Effect of High Intensity Focused Ultrasound Keratoplasty on Rabbit Anterior SegmentMenglei Wang0Meixuan Li1Pisong Yan2Qiang Luo3Yu Zhang4Zhiyu Du5Department of Ophthalmology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, ChinaDepartment of Ophthalmology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, ChinaMedal Eye Institute, Chongqing 400050, ChinaKey Laboratory of Molecular Biology for Infectious Diseases (Ministry of Education), Institute for Viral Hepatitis, Department of Infectious Diseases, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, ChinaDepartment of Ophthalmology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, ChinaDepartment of Ophthalmology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, ChinaPurpose. To evaluate the safety of high-intensity focused ultrasound keratoplasty as a treatment for presbyopia by examining its effect on the rabbit anterior segment. Methods. The right corneas of 36 New Zealand rabbits were treated with HIFU keratoplasty. The animals were sacrificed at 1, 7, 15, 30, 60, and 90 days after operation. Collagen type I, MMP-2, and MMP-9 were evaluated using immunohistochemistry. For the detection of apoptosis, the TUNEL method was applied. The SOD and MDA levels were analyzed with assay kits. Results. Collagen type I, MMP-2, and MMP-9 levels were altered after the operation but returned to normal within 90 days. The apoptotic index (AI) of the corneal cells decreased from 1 to 30 days gradually. No apoptosis was observed in the epithelial cells of the lens, and the SOD and MDA levels were normal at any time point. Conclusion. After HIFU keratoplasty, the histomorphology of the cornea changed, the corneal collagen type I levels decreased, the corneal MMP-2 and MMP-9 levels increased, and the corneal cells underwent apoptosis for a period of time. Ninety days after the operation, the levels returned to normal, and the lenses were not affected. Thus, HIFU presents good biological safety for eyes.http://dx.doi.org/10.1155/2017/6067890 |
| spellingShingle | Menglei Wang Meixuan Li Pisong Yan Qiang Luo Yu Zhang Zhiyu Du The Effect of High Intensity Focused Ultrasound Keratoplasty on Rabbit Anterior Segment Journal of Ophthalmology |
| title | The Effect of High Intensity Focused Ultrasound Keratoplasty on Rabbit Anterior Segment |
| title_full | The Effect of High Intensity Focused Ultrasound Keratoplasty on Rabbit Anterior Segment |
| title_fullStr | The Effect of High Intensity Focused Ultrasound Keratoplasty on Rabbit Anterior Segment |
| title_full_unstemmed | The Effect of High Intensity Focused Ultrasound Keratoplasty on Rabbit Anterior Segment |
| title_short | The Effect of High Intensity Focused Ultrasound Keratoplasty on Rabbit Anterior Segment |
| title_sort | effect of high intensity focused ultrasound keratoplasty on rabbit anterior segment |
| url | http://dx.doi.org/10.1155/2017/6067890 |
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