Dental pulp stem cell-derived intracellular vesicles prevent orthodontic relapse by inhibiting PI3K/Akt/NF-κB-mediated osteoclast activity
Abstract Background Orthodontic relapse, the undesired deviation of teeth from their corrected positions, remains a significant challenge in clinical orthodontics. Incomplete periodontal bone remodeling has been identified as a key factor in this process. Despite decades of research, currently there...
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-01-01
|
| Series: | Stem Cell Research & Therapy |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13287-025-04146-3 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832586023524106240 |
|---|---|
| author | Boyuan Peng Ziwei Li Yong Cheng Henghua Jiang Qingsong Ye Guangli Han |
| author_facet | Boyuan Peng Ziwei Li Yong Cheng Henghua Jiang Qingsong Ye Guangli Han |
| author_sort | Boyuan Peng |
| collection | DOAJ |
| description | Abstract Background Orthodontic relapse, the undesired deviation of teeth from their corrected positions, remains a significant challenge in clinical orthodontics. Incomplete periodontal bone remodeling has been identified as a key factor in this process. Despite decades of research, currently there are no effective strategies to prevent relapse. Methods We isolated and identified dental pulp stem cell-derived intracellular vesicles (DPSC-IV) from human dental pulp tissue. To investigate its effect, DPSC-IV was added to osteoblast or osteoclast differentiation medium. During the orthodontic retention period, DPSC-IV was administrated to rats by subgingival injection. Relapse distance and relapse rate were calculated to evaluate DPSC-IV’s ability to prevent relapse. Additionally, Western blot analysis were used to examine DPSC-IV’s inhibitory effect on osteoclast differentiation. Results DPSC-IV significantly promoted osteoblast differentiation and inhibited osteoclast differentiation. Application of DPSC-IV during retention resulted in a significant reduction in both relapse distance and relapse rate, with improved periodontal structure and decreased osteoclast activity. This effect was mediated by the PI3K/Akt/NF-κB signaling pathway and could be reversed by the PI3K activator insulin-like growth factor-1 (IGF-1). Conclusion This study highlights the potential of DPSC-IV as a novel preventive approach against orthodontic relapse, offering a novel strategy for maintaining long-term orthodontic stability. Graphical Abstract |
| format | Article |
| id | doaj-art-e0bd93c58e6f4713814da8d455b81b53 |
| institution | Kabale University |
| issn | 1757-6512 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Stem Cell Research & Therapy |
| spelling | doaj-art-e0bd93c58e6f4713814da8d455b81b532025-01-26T12:18:08ZengBMCStem Cell Research & Therapy1757-65122025-01-0116111410.1186/s13287-025-04146-3Dental pulp stem cell-derived intracellular vesicles prevent orthodontic relapse by inhibiting PI3K/Akt/NF-κB-mediated osteoclast activityBoyuan Peng0Ziwei Li1Yong Cheng2Henghua Jiang3Qingsong Ye4Guangli Han5State Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan UniversityCenter of Regenerative Medicine, Department of Stomatology, Renmin Hospital of Wuhan UniversityState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan UniversityState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan UniversityCenter of Regenerative Medicine, Department of Stomatology, Renmin Hospital of Wuhan UniversityState Key Laboratory of Oral & Maxillofacial Reconstruction and Regeneration, Key Laboratory of Oral Biomedicine Ministry of Education, Hubei Key Laboratory of Stomatology, School & Hospital of Stomatology, Wuhan UniversityAbstract Background Orthodontic relapse, the undesired deviation of teeth from their corrected positions, remains a significant challenge in clinical orthodontics. Incomplete periodontal bone remodeling has been identified as a key factor in this process. Despite decades of research, currently there are no effective strategies to prevent relapse. Methods We isolated and identified dental pulp stem cell-derived intracellular vesicles (DPSC-IV) from human dental pulp tissue. To investigate its effect, DPSC-IV was added to osteoblast or osteoclast differentiation medium. During the orthodontic retention period, DPSC-IV was administrated to rats by subgingival injection. Relapse distance and relapse rate were calculated to evaluate DPSC-IV’s ability to prevent relapse. Additionally, Western blot analysis were used to examine DPSC-IV’s inhibitory effect on osteoclast differentiation. Results DPSC-IV significantly promoted osteoblast differentiation and inhibited osteoclast differentiation. Application of DPSC-IV during retention resulted in a significant reduction in both relapse distance and relapse rate, with improved periodontal structure and decreased osteoclast activity. This effect was mediated by the PI3K/Akt/NF-κB signaling pathway and could be reversed by the PI3K activator insulin-like growth factor-1 (IGF-1). Conclusion This study highlights the potential of DPSC-IV as a novel preventive approach against orthodontic relapse, offering a novel strategy for maintaining long-term orthodontic stability. Graphical Abstracthttps://doi.org/10.1186/s13287-025-04146-3Orthodontic tooth movementIntercellular vesiclesBone remodelingOral medicineStem cellsCell communication |
| spellingShingle | Boyuan Peng Ziwei Li Yong Cheng Henghua Jiang Qingsong Ye Guangli Han Dental pulp stem cell-derived intracellular vesicles prevent orthodontic relapse by inhibiting PI3K/Akt/NF-κB-mediated osteoclast activity Stem Cell Research & Therapy Orthodontic tooth movement Intercellular vesicles Bone remodeling Oral medicine Stem cells Cell communication |
| title | Dental pulp stem cell-derived intracellular vesicles prevent orthodontic relapse by inhibiting PI3K/Akt/NF-κB-mediated osteoclast activity |
| title_full | Dental pulp stem cell-derived intracellular vesicles prevent orthodontic relapse by inhibiting PI3K/Akt/NF-κB-mediated osteoclast activity |
| title_fullStr | Dental pulp stem cell-derived intracellular vesicles prevent orthodontic relapse by inhibiting PI3K/Akt/NF-κB-mediated osteoclast activity |
| title_full_unstemmed | Dental pulp stem cell-derived intracellular vesicles prevent orthodontic relapse by inhibiting PI3K/Akt/NF-κB-mediated osteoclast activity |
| title_short | Dental pulp stem cell-derived intracellular vesicles prevent orthodontic relapse by inhibiting PI3K/Akt/NF-κB-mediated osteoclast activity |
| title_sort | dental pulp stem cell derived intracellular vesicles prevent orthodontic relapse by inhibiting pi3k akt nf κb mediated osteoclast activity |
| topic | Orthodontic tooth movement Intercellular vesicles Bone remodeling Oral medicine Stem cells Cell communication |
| url | https://doi.org/10.1186/s13287-025-04146-3 |
| work_keys_str_mv | AT boyuanpeng dentalpulpstemcellderivedintracellularvesiclespreventorthodonticrelapsebyinhibitingpi3kaktnfkbmediatedosteoclastactivity AT ziweili dentalpulpstemcellderivedintracellularvesiclespreventorthodonticrelapsebyinhibitingpi3kaktnfkbmediatedosteoclastactivity AT yongcheng dentalpulpstemcellderivedintracellularvesiclespreventorthodonticrelapsebyinhibitingpi3kaktnfkbmediatedosteoclastactivity AT henghuajiang dentalpulpstemcellderivedintracellularvesiclespreventorthodonticrelapsebyinhibitingpi3kaktnfkbmediatedosteoclastactivity AT qingsongye dentalpulpstemcellderivedintracellularvesiclespreventorthodonticrelapsebyinhibitingpi3kaktnfkbmediatedosteoclastactivity AT guanglihan dentalpulpstemcellderivedintracellularvesiclespreventorthodonticrelapsebyinhibitingpi3kaktnfkbmediatedosteoclastactivity |