Evaluation of Ternary Mobile Phases for the Analysis of Carbonyl Compound Derivatives Using High-Performance Liquid Chromatography
In this study, the feasibility of ternary mobile phases was examined in a high-performance liquid chromatography (HPLC)-based analysis of carbonyl compounds (CCs). To test the performance of different ternary phases, the liquid phase standards containing a 15 aldehyde/ketone-DNPH(o) mix were analyze...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/tsw.2011.4 |
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| author | Duy Xuan Ho Ki-Hyun Kim |
| author_facet | Duy Xuan Ho Ki-Hyun Kim |
| author_sort | Duy Xuan Ho |
| collection | DOAJ |
| description | In this study, the feasibility of ternary mobile phases was examined in a high-performance liquid chromatography (HPLC)-based analysis of carbonyl compounds (CCs). To test the performance of different ternary phases, the liquid phase standards containing a 15 aldehyde/ketone-DNPH(o) mix were analyzed through a series of five-point calibration experiments. For this comparison, three types of ternary mobile phases were prepared initially by mixing water (W) with two of the following three organic solvents: isopropanol (I), methanol (M), and tetrahydrofuran (T). The resulting three types of ternary phases (named as WIM, WTM, and WIT) were tested and evaluated in relation to the water content or in terms of methanol-to-water ratio (M/W). The results derived by the three ternary phases revealed that the optimal resolution was attained near maximum water content, while those of WIT consistently suffered from poor resolution problems. The relative performances of WIM and WTM phases, if assessed by three key operating parameters (sensitivity, retention time, and resolution), were found to be reliable for most selected CCs with the decreasing M/W ratio. |
| format | Article |
| id | doaj-art-e0b44f73203648d6b912fde04e4e4cbc |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-e0b44f73203648d6b912fde04e4e4cbc2025-08-20T03:25:59ZengWileyThe Scientific World Journal1537-744X2011-01-011111910.1100/tsw.2011.4Evaluation of Ternary Mobile Phases for the Analysis of Carbonyl Compound Derivatives Using High-Performance Liquid ChromatographyDuy Xuan Ho0Ki-Hyun Kim1Department of Environment and Energy, Sejong University, Seoul, KoreaDepartment of Environment and Energy, Sejong University, Seoul, KoreaIn this study, the feasibility of ternary mobile phases was examined in a high-performance liquid chromatography (HPLC)-based analysis of carbonyl compounds (CCs). To test the performance of different ternary phases, the liquid phase standards containing a 15 aldehyde/ketone-DNPH(o) mix were analyzed through a series of five-point calibration experiments. For this comparison, three types of ternary mobile phases were prepared initially by mixing water (W) with two of the following three organic solvents: isopropanol (I), methanol (M), and tetrahydrofuran (T). The resulting three types of ternary phases (named as WIM, WTM, and WIT) were tested and evaluated in relation to the water content or in terms of methanol-to-water ratio (M/W). The results derived by the three ternary phases revealed that the optimal resolution was attained near maximum water content, while those of WIT consistently suffered from poor resolution problems. The relative performances of WIM and WTM phases, if assessed by three key operating parameters (sensitivity, retention time, and resolution), were found to be reliable for most selected CCs with the decreasing M/W ratio.http://dx.doi.org/10.1100/tsw.2011.4 |
| spellingShingle | Duy Xuan Ho Ki-Hyun Kim Evaluation of Ternary Mobile Phases for the Analysis of Carbonyl Compound Derivatives Using High-Performance Liquid Chromatography The Scientific World Journal |
| title | Evaluation of Ternary Mobile Phases for the Analysis of Carbonyl Compound Derivatives Using High-Performance Liquid Chromatography |
| title_full | Evaluation of Ternary Mobile Phases for the Analysis of Carbonyl Compound Derivatives Using High-Performance Liquid Chromatography |
| title_fullStr | Evaluation of Ternary Mobile Phases for the Analysis of Carbonyl Compound Derivatives Using High-Performance Liquid Chromatography |
| title_full_unstemmed | Evaluation of Ternary Mobile Phases for the Analysis of Carbonyl Compound Derivatives Using High-Performance Liquid Chromatography |
| title_short | Evaluation of Ternary Mobile Phases for the Analysis of Carbonyl Compound Derivatives Using High-Performance Liquid Chromatography |
| title_sort | evaluation of ternary mobile phases for the analysis of carbonyl compound derivatives using high performance liquid chromatography |
| url | http://dx.doi.org/10.1100/tsw.2011.4 |
| work_keys_str_mv | AT duyxuanho evaluationofternarymobilephasesfortheanalysisofcarbonylcompoundderivativesusinghighperformanceliquidchromatography AT kihyunkim evaluationofternarymobilephasesfortheanalysisofcarbonylcompoundderivativesusinghighperformanceliquidchromatography |