The Suppression of Signal Transducer and Activator of Transcription-3 in A549 human Lung Carcinoma Cells Induced by Marine Sponge Callyspongia aerizusa
Yuni Elsa Hadisaputri,1,* Annida Adha Nurhaniefah,1,* Mutakin Mutakin,2 Rini Hendriani,3 Andri Rezano,4 Iyan Sopyan,5 Yusnaini Yusnaini,6 Yonathan Asikin,7 Rizky Abdulah3 1Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor, Indonesia; 2Depa...
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Dove Medical Press
2025-01-01
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| author | Hadisaputri YE Nurhaniefah AA Mutakin M Hendriani R Rezano A Sopyan I Yusnaini Y Asikin Y Abdulah R |
| author_facet | Hadisaputri YE Nurhaniefah AA Mutakin M Hendriani R Rezano A Sopyan I Yusnaini Y Asikin Y Abdulah R |
| author_sort | Hadisaputri YE |
| collection | DOAJ |
| description | Yuni Elsa Hadisaputri,1,* Annida Adha Nurhaniefah,1,* Mutakin Mutakin,2 Rini Hendriani,3 Andri Rezano,4 Iyan Sopyan,5 Yusnaini Yusnaini,6 Yonathan Asikin,7 Rizky Abdulah3 1Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor, Indonesia; 2Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor, Indonesia; 3Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor, Indonesia; 4Department of Biomedical Sciences, Cell Biology Division, Faculty of Medicine, Universitas Padjadjaran, Jatinangor, Indonesia; 5Department of Pharmaceutical and Pharmacy Technology, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor, Indonesia; 6Faculty of Fisheries and Marine Sciences, Universitas Halu Oleo, Kendari, Indonesia; 7Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Okinawa, Japan*These authors contributed equally to this workCorrespondence: Yuni Elsa Hadisaputri, Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang KM.21, Jatinangor, West Java, 45363, Indonesia, Tel +62-22-84288888 Ext. 3510, Email yuni.elsa@unpad.ac.idIntroduction: Lung cancer is recognized as a highly lethal disease, demanding swift and accurate solutions. Previous analysis showed the cytotoxic impact of Callyspongia aerizusa (C. aerizusa) extract containing ergost-22-en-3-one and ergost-7-en3-ol against A549 lung cancer cells, with an IC50 value of 9.38 μg/mL. However, the extract did not have cytotoxicity towards Het-1A esophagus epithelial cells. Several reviews also validated the upregulation of pro-apoptotic molecules and the inhibition of anti-apoptotic molecules linked to the caspase-dependent signaling pathway.Purpose: The objective of this research was to extend the understanding of the effects of C. aerizusa extract on A549 lung carcinoma, examining its influence on various signaling pathways, malignancy, migration, and invasion.Materials and Methods: PCR was used to measure mRNA expression, targeting PTEN, Akt, mTOR, STAT-3, IL-6, VEGF, and HIF1α. Additionally, Western Blot analysis was adopted to assess PTEN, p-Akt, Akt, p-mTOR, and p-STAT-3 protein expressions. Wound healing and invasion assays were performed to measure the migration and invasion capabilities of A549 cells post-treatment with C. aerizusa extract.Results: The mRNA expression analysis showed an increase in Akt and m-TOR but a decrease in PTEN and STAT-3 after 24 hours of treatment with C. aerizusa extract. At the protein level, there was a downregulation of p-Akt, Akt, p-mTOR, and p-STAT-3, while PTEN increased during 24-hour treatment. Wound healing and invasion assay results showed a weakened ability of A549 cells after a 24-hour treatment with C. aerizusa extract. Moreover, IL-6 and HIF-1α mRNA expression levels decreased during 24 hours, while VEGF mRNA had a slight decrease compared to untreated cells.Conclusion: In conclusion, the ergosteroids present in marine sponge C. aerizusa extract signified a remarkable reduction in malignancy, migration, and invasion capabilities in A549 lung carcinoma cells. These results suggested their promising candidacy for future anti-angiogenesis in anticancer therapy.Keywords: Callyspongia aerizusa, A549 cells, PI3K/Akt signaling, STAT-3, wound healing, invasion |
| format | Article |
| id | doaj-art-e08ee3d7ddc0445cbb4e6c8be6707173 |
| institution | OA Journals |
| issn | 1179-1454 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | Journal of Experimental Pharmacology |
| spelling | doaj-art-e08ee3d7ddc0445cbb4e6c8be67071732025-08-20T02:35:46ZengDove Medical PressJournal of Experimental Pharmacology1179-14542025-01-01Volume 17152599192The Suppression of Signal Transducer and Activator of Transcription-3 in A549 human Lung Carcinoma Cells Induced by Marine Sponge Callyspongia aerizusaHadisaputri YENurhaniefah AAMutakin MHendriani RRezano ASopyan IYusnaini YAsikin YAbdulah RYuni Elsa Hadisaputri,1,* Annida Adha Nurhaniefah,1,* Mutakin Mutakin,2 Rini Hendriani,3 Andri Rezano,4 Iyan Sopyan,5 Yusnaini Yusnaini,6 Yonathan Asikin,7 Rizky Abdulah3 1Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor, Indonesia; 2Department of Pharmaceutical Analysis and Medicinal Chemistry, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor, Indonesia; 3Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor, Indonesia; 4Department of Biomedical Sciences, Cell Biology Division, Faculty of Medicine, Universitas Padjadjaran, Jatinangor, Indonesia; 5Department of Pharmaceutical and Pharmacy Technology, Faculty of Pharmacy, Universitas Padjadjaran, Jatinangor, Indonesia; 6Faculty of Fisheries and Marine Sciences, Universitas Halu Oleo, Kendari, Indonesia; 7Department of Bioscience and Biotechnology, Faculty of Agriculture, University of the Ryukyus, Okinawa, Japan*These authors contributed equally to this workCorrespondence: Yuni Elsa Hadisaputri, Department of Pharmaceutical Biology, Faculty of Pharmacy, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang KM.21, Jatinangor, West Java, 45363, Indonesia, Tel +62-22-84288888 Ext. 3510, Email yuni.elsa@unpad.ac.idIntroduction: Lung cancer is recognized as a highly lethal disease, demanding swift and accurate solutions. Previous analysis showed the cytotoxic impact of Callyspongia aerizusa (C. aerizusa) extract containing ergost-22-en-3-one and ergost-7-en3-ol against A549 lung cancer cells, with an IC50 value of 9.38 μg/mL. However, the extract did not have cytotoxicity towards Het-1A esophagus epithelial cells. Several reviews also validated the upregulation of pro-apoptotic molecules and the inhibition of anti-apoptotic molecules linked to the caspase-dependent signaling pathway.Purpose: The objective of this research was to extend the understanding of the effects of C. aerizusa extract on A549 lung carcinoma, examining its influence on various signaling pathways, malignancy, migration, and invasion.Materials and Methods: PCR was used to measure mRNA expression, targeting PTEN, Akt, mTOR, STAT-3, IL-6, VEGF, and HIF1α. Additionally, Western Blot analysis was adopted to assess PTEN, p-Akt, Akt, p-mTOR, and p-STAT-3 protein expressions. Wound healing and invasion assays were performed to measure the migration and invasion capabilities of A549 cells post-treatment with C. aerizusa extract.Results: The mRNA expression analysis showed an increase in Akt and m-TOR but a decrease in PTEN and STAT-3 after 24 hours of treatment with C. aerizusa extract. At the protein level, there was a downregulation of p-Akt, Akt, p-mTOR, and p-STAT-3, while PTEN increased during 24-hour treatment. Wound healing and invasion assay results showed a weakened ability of A549 cells after a 24-hour treatment with C. aerizusa extract. Moreover, IL-6 and HIF-1α mRNA expression levels decreased during 24 hours, while VEGF mRNA had a slight decrease compared to untreated cells.Conclusion: In conclusion, the ergosteroids present in marine sponge C. aerizusa extract signified a remarkable reduction in malignancy, migration, and invasion capabilities in A549 lung carcinoma cells. These results suggested their promising candidacy for future anti-angiogenesis in anticancer therapy.Keywords: Callyspongia aerizusa, A549 cells, PI3K/Akt signaling, STAT-3, wound healing, invasionhttps://www.dovepress.com/the-suppression-of-signal-transducer-and-activator-of-transcription-3--peer-reviewed-fulltext-article-JEPcallyspongia aerizusaa549 cellspi3k/akt signalingstat-3wound healinginvasion |
| spellingShingle | Hadisaputri YE Nurhaniefah AA Mutakin M Hendriani R Rezano A Sopyan I Yusnaini Y Asikin Y Abdulah R The Suppression of Signal Transducer and Activator of Transcription-3 in A549 human Lung Carcinoma Cells Induced by Marine Sponge Callyspongia aerizusa Journal of Experimental Pharmacology callyspongia aerizusa a549 cells pi3k/akt signaling stat-3 wound healing invasion |
| title | The Suppression of Signal Transducer and Activator of Transcription-3 in A549 human Lung Carcinoma Cells Induced by Marine Sponge Callyspongia aerizusa |
| title_full | The Suppression of Signal Transducer and Activator of Transcription-3 in A549 human Lung Carcinoma Cells Induced by Marine Sponge Callyspongia aerizusa |
| title_fullStr | The Suppression of Signal Transducer and Activator of Transcription-3 in A549 human Lung Carcinoma Cells Induced by Marine Sponge Callyspongia aerizusa |
| title_full_unstemmed | The Suppression of Signal Transducer and Activator of Transcription-3 in A549 human Lung Carcinoma Cells Induced by Marine Sponge Callyspongia aerizusa |
| title_short | The Suppression of Signal Transducer and Activator of Transcription-3 in A549 human Lung Carcinoma Cells Induced by Marine Sponge Callyspongia aerizusa |
| title_sort | suppression of signal transducer and activator of transcription 3 in a549 nbsp human lung carcinoma cells induced by marine sponge callyspongia aerizusa |
| topic | callyspongia aerizusa a549 cells pi3k/akt signaling stat-3 wound healing invasion |
| url | https://www.dovepress.com/the-suppression-of-signal-transducer-and-activator-of-transcription-3--peer-reviewed-fulltext-article-JEP |
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