The Possible Role of Neutrophils in the Induction of Osteoclastogenesis
The ligand of the receptor activator of NF-κB (RANKL) is a key molecule in the formation of osteoclasts, the key cells that cause the disease-associated alveolar bone resorption in periodontitis. We hypothesized that polymorphonuclear leukocytes (PMNs), found as the most prominent cells of inflamed...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2019/8672604 |
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| author | Carolyn G. J. Moonen Teun J. de Vries Patrick Rijkschroeff Patrice E. Poubelle Elena A. Nicu Bruno G. Loos |
| author_facet | Carolyn G. J. Moonen Teun J. de Vries Patrick Rijkschroeff Patrice E. Poubelle Elena A. Nicu Bruno G. Loos |
| author_sort | Carolyn G. J. Moonen |
| collection | DOAJ |
| description | The ligand of the receptor activator of NF-κB (RANKL) is a key molecule in the formation of osteoclasts, the key cells that cause the disease-associated alveolar bone resorption in periodontitis. We hypothesized that polymorphonuclear leukocytes (PMNs), found as the most prominent cells of inflamed periodontal tissues, could play an important role in providing signals to trigger osteoclastogenesis and thus activating pathological bone resorption in periodontitis. RANKL expression was investigated on circulatory PMNs (cPMNs) and oral PMNs (oPMNs) taken from both controls and periodontitis patients. On average, 2.3% and 2.4% RANKL expression was detected on the cPMNs and oPMNs from periodontitis patients, which did not differ significantly from healthy controls. Since cPMNs may acquire a more osteoclastogenesis-facilitating phenotype while migrating into the inflamed periodontium, we next investigated whether stimulated (with LPS, TNF-α, or IL-6) cPMNs have the capacity to contribute to osteoclastogenesis. Enduring surface expression of RANKL for short-lived cells as cPMNs was achieved by fixating stimulated cPMNs. RANKL expression on stimulated cPMNs, as assessed by flow cytometry and immunohistochemistry, was limited (6.48±0.72%, mean expression±SEM) after 24 and 48 hours of stimulation with LPS. Likewise, stimulation with TNF-α and IL-6 resulted in limited RANKL expression levels. These limited levels of expression did not induce osteoclastogenesis when cocultured with preosteoclasts for 10 days. We report that, under the aforementioned experimental conditions, neither cPMNs nor oPMNs directly induced osteoclastogenesis. Further elucidation of the key cellular players and immune mediators that stimulate alveolar bone resorption in periodontitis will help to unravel its pathogenesis. |
| format | Article |
| id | doaj-art-e0784bcbcb0f433184ca58077adea348 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-e0784bcbcb0f433184ca58077adea3482025-02-03T05:48:07ZengWileyJournal of Immunology Research2314-88612314-71562019-01-01201910.1155/2019/86726048672604The Possible Role of Neutrophils in the Induction of OsteoclastogenesisCarolyn G. J. Moonen0Teun J. de Vries1Patrick Rijkschroeff2Patrice E. Poubelle3Elena A. Nicu4Bruno G. Loos5Department of Periodontology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, NetherlandsDepartment of Periodontology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, NetherlandsDepartment of Periodontology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, NetherlandsDepartment of Medicine, Centre de Recherche en Rhumatologie et Immunologie (CRRI), Centre Hospitalier de l’Université Laval (CRCHUL), Faculty of Medicine, Université Laval, Québec, QC, CanadaDepartment of Periodontology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, NetherlandsDepartment of Periodontology, Academic Centre for Dentistry Amsterdam (ACTA), University of Amsterdam and Vrije Universiteit Amsterdam, Amsterdam, NetherlandsThe ligand of the receptor activator of NF-κB (RANKL) is a key molecule in the formation of osteoclasts, the key cells that cause the disease-associated alveolar bone resorption in periodontitis. We hypothesized that polymorphonuclear leukocytes (PMNs), found as the most prominent cells of inflamed periodontal tissues, could play an important role in providing signals to trigger osteoclastogenesis and thus activating pathological bone resorption in periodontitis. RANKL expression was investigated on circulatory PMNs (cPMNs) and oral PMNs (oPMNs) taken from both controls and periodontitis patients. On average, 2.3% and 2.4% RANKL expression was detected on the cPMNs and oPMNs from periodontitis patients, which did not differ significantly from healthy controls. Since cPMNs may acquire a more osteoclastogenesis-facilitating phenotype while migrating into the inflamed periodontium, we next investigated whether stimulated (with LPS, TNF-α, or IL-6) cPMNs have the capacity to contribute to osteoclastogenesis. Enduring surface expression of RANKL for short-lived cells as cPMNs was achieved by fixating stimulated cPMNs. RANKL expression on stimulated cPMNs, as assessed by flow cytometry and immunohistochemistry, was limited (6.48±0.72%, mean expression±SEM) after 24 and 48 hours of stimulation with LPS. Likewise, stimulation with TNF-α and IL-6 resulted in limited RANKL expression levels. These limited levels of expression did not induce osteoclastogenesis when cocultured with preosteoclasts for 10 days. We report that, under the aforementioned experimental conditions, neither cPMNs nor oPMNs directly induced osteoclastogenesis. Further elucidation of the key cellular players and immune mediators that stimulate alveolar bone resorption in periodontitis will help to unravel its pathogenesis.http://dx.doi.org/10.1155/2019/8672604 |
| spellingShingle | Carolyn G. J. Moonen Teun J. de Vries Patrick Rijkschroeff Patrice E. Poubelle Elena A. Nicu Bruno G. Loos The Possible Role of Neutrophils in the Induction of Osteoclastogenesis Journal of Immunology Research |
| title | The Possible Role of Neutrophils in the Induction of Osteoclastogenesis |
| title_full | The Possible Role of Neutrophils in the Induction of Osteoclastogenesis |
| title_fullStr | The Possible Role of Neutrophils in the Induction of Osteoclastogenesis |
| title_full_unstemmed | The Possible Role of Neutrophils in the Induction of Osteoclastogenesis |
| title_short | The Possible Role of Neutrophils in the Induction of Osteoclastogenesis |
| title_sort | possible role of neutrophils in the induction of osteoclastogenesis |
| url | http://dx.doi.org/10.1155/2019/8672604 |
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