A Review of the Utility of Established Cell Lines for Isolation and Propagation of the Southern African Territories Serotypes of Foot-and-Mouth Disease Virus
Cell culture underpins virus isolation and virus neutralisation tests, which are both gold-standard diagnostic methods for foot-and-mouth disease (FMD). Cell culture is also crucial for the propagation of inactivated foot-and-mouth disease virus (FMDV) vaccines. Both primary cells and cell lines are...
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2024-12-01
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| author | Kitsiso Gaboiphiwe Tshephang Iris Kabelo Petronella Thato Mosholombe Joseph Hyera Elliot Mpolokang Fana Kabo Masisi Kebaneilwe Lebani |
| author_facet | Kitsiso Gaboiphiwe Tshephang Iris Kabelo Petronella Thato Mosholombe Joseph Hyera Elliot Mpolokang Fana Kabo Masisi Kebaneilwe Lebani |
| author_sort | Kitsiso Gaboiphiwe |
| collection | DOAJ |
| description | Cell culture underpins virus isolation and virus neutralisation tests, which are both gold-standard diagnostic methods for foot-and-mouth disease (FMD). Cell culture is also crucial for the propagation of inactivated foot-and-mouth disease virus (FMDV) vaccines. Both primary cells and cell lines are utilised in FMDV isolation and propagation. Widely used cell lines for FMDV and isolation and propagation include baby hamster kidney cells (BHK-21), swine kidney cells (IB-RS-2), foetal goat tongue (ZZ-R 127), foetal porcine kidney cells (LFBKvB6), bovine kidney cells (BK), human telomerase reverse transcriptase bovine thyroid (hTERT-BTY) and porcine kidney-originating PK-15 or SK 6 cell lines. This review highlights how different receptors and molecules—integrins, heparan sulphate (HS), and the Jumonji C-domain containing Protein 6 (JMJD6)—found on the surface of different cell types contribute to differences experienced with susceptibility and sensitivity of the cells to infection with different serotypes of FMDV. This review specifically focuses on Southern African territory (SAT) serotypes, which are unique to the Southern African context and are often under-investigated in cell line development for FMDV isolation and propagation. |
| format | Article |
| id | doaj-art-e0738b2a21f24402a88f9fb3c2f12001 |
| institution | Kabale University |
| issn | 1999-4915 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Viruses |
| spelling | doaj-art-e0738b2a21f24402a88f9fb3c2f120012025-01-24T13:52:21ZengMDPI AGViruses1999-49152024-12-011713910.3390/v17010039A Review of the Utility of Established Cell Lines for Isolation and Propagation of the Southern African Territories Serotypes of Foot-and-Mouth Disease VirusKitsiso Gaboiphiwe0Tshephang Iris Kabelo1Petronella Thato Mosholombe2Joseph Hyera3Elliot Mpolokang Fana4Kabo Masisi5Kebaneilwe Lebani6World Organisation for Animal Health (WOAH) Foot-and-Mouth Disease Reference Laboratory, Botswana Vaccine Institute, Private Bag 0031, Gaborone, BotswanaDepartment of Biological Sciences and Biotechnology, School of Life Sciences, Botswana International University of Science and Technology, Private Bag 16, Palapye 10071, BotswanaDepartment of Biological Sciences and Biotechnology, School of Life Sciences, Botswana International University of Science and Technology, Private Bag 16, Palapye 10071, BotswanaWorld Organisation for Animal Health (WOAH) Foot-and-Mouth Disease Reference Laboratory, Botswana Vaccine Institute, Private Bag 0031, Gaborone, BotswanaWorld Organisation for Animal Health (WOAH) Foot-and-Mouth Disease Reference Laboratory, Botswana Vaccine Institute, Private Bag 0031, Gaborone, BotswanaDepartment of Biological Sciences and Biotechnology, School of Life Sciences, Botswana International University of Science and Technology, Private Bag 16, Palapye 10071, BotswanaDepartment of Biological Sciences and Biotechnology, School of Life Sciences, Botswana International University of Science and Technology, Private Bag 16, Palapye 10071, BotswanaCell culture underpins virus isolation and virus neutralisation tests, which are both gold-standard diagnostic methods for foot-and-mouth disease (FMD). Cell culture is also crucial for the propagation of inactivated foot-and-mouth disease virus (FMDV) vaccines. Both primary cells and cell lines are utilised in FMDV isolation and propagation. Widely used cell lines for FMDV and isolation and propagation include baby hamster kidney cells (BHK-21), swine kidney cells (IB-RS-2), foetal goat tongue (ZZ-R 127), foetal porcine kidney cells (LFBKvB6), bovine kidney cells (BK), human telomerase reverse transcriptase bovine thyroid (hTERT-BTY) and porcine kidney-originating PK-15 or SK 6 cell lines. This review highlights how different receptors and molecules—integrins, heparan sulphate (HS), and the Jumonji C-domain containing Protein 6 (JMJD6)—found on the surface of different cell types contribute to differences experienced with susceptibility and sensitivity of the cells to infection with different serotypes of FMDV. This review specifically focuses on Southern African territory (SAT) serotypes, which are unique to the Southern African context and are often under-investigated in cell line development for FMDV isolation and propagation.https://www.mdpi.com/1999-4915/17/1/39foot-and-mouth disease virus (FMDV)foot-and-mouth disease (FMD)SAT serotypescell linesFMD vaccine productionFMD diagnostic |
| spellingShingle | Kitsiso Gaboiphiwe Tshephang Iris Kabelo Petronella Thato Mosholombe Joseph Hyera Elliot Mpolokang Fana Kabo Masisi Kebaneilwe Lebani A Review of the Utility of Established Cell Lines for Isolation and Propagation of the Southern African Territories Serotypes of Foot-and-Mouth Disease Virus Viruses foot-and-mouth disease virus (FMDV) foot-and-mouth disease (FMD) SAT serotypes cell lines FMD vaccine production FMD diagnostic |
| title | A Review of the Utility of Established Cell Lines for Isolation and Propagation of the Southern African Territories Serotypes of Foot-and-Mouth Disease Virus |
| title_full | A Review of the Utility of Established Cell Lines for Isolation and Propagation of the Southern African Territories Serotypes of Foot-and-Mouth Disease Virus |
| title_fullStr | A Review of the Utility of Established Cell Lines for Isolation and Propagation of the Southern African Territories Serotypes of Foot-and-Mouth Disease Virus |
| title_full_unstemmed | A Review of the Utility of Established Cell Lines for Isolation and Propagation of the Southern African Territories Serotypes of Foot-and-Mouth Disease Virus |
| title_short | A Review of the Utility of Established Cell Lines for Isolation and Propagation of the Southern African Territories Serotypes of Foot-and-Mouth Disease Virus |
| title_sort | review of the utility of established cell lines for isolation and propagation of the southern african territories serotypes of foot and mouth disease virus |
| topic | foot-and-mouth disease virus (FMDV) foot-and-mouth disease (FMD) SAT serotypes cell lines FMD vaccine production FMD diagnostic |
| url | https://www.mdpi.com/1999-4915/17/1/39 |
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