Molecular cloning, expression and functional analysis of foxl2 from Chinese sturgeon (Acipenser sinensis) in relation to sex differentiation
IntroductionOur understanding of the regulatory mechanisms that govern the development of sexual characteristics in sturgeon, particularly the Chinese sturgeon (Acipenser sinensis), is limited. Foxl2, a member of the forkhead/HNF-3-related transcription factor family, is known for its role in precis...
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Frontiers Media S.A.
2024-12-01
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| Series: | Frontiers in Marine Science |
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| author | Yacheng Hu Yacheng Hu Ruihua Tan Yang Li Yang Li Tingting Shu Tingting Shu Gang Yang Zhong Chu Haoran Wang Xueqing Liu Xueqing Liu Xin Zhu Xin Zhu Binzhong Wang Binzhong Wang Baifu Guo Baifu Guo Runqiu Wang Runqiu Wang Huantao Qu Huantao Qu Haoyong Song Haoyong Song Dezhi Zhang Dezhi Zhang Hejun Du Hejun Du Zhiyuan Li Zhiyuan Li Jianbo Chang Jianbo Chang Dianchang Wang Dan Xu Wei Jiang Wei Jiang Qingfei Li Yuanjin Yang Yuanjin Yang |
| author_facet | Yacheng Hu Yacheng Hu Ruihua Tan Yang Li Yang Li Tingting Shu Tingting Shu Gang Yang Zhong Chu Haoran Wang Xueqing Liu Xueqing Liu Xin Zhu Xin Zhu Binzhong Wang Binzhong Wang Baifu Guo Baifu Guo Runqiu Wang Runqiu Wang Huantao Qu Huantao Qu Haoyong Song Haoyong Song Dezhi Zhang Dezhi Zhang Hejun Du Hejun Du Zhiyuan Li Zhiyuan Li Jianbo Chang Jianbo Chang Dianchang Wang Dan Xu Wei Jiang Wei Jiang Qingfei Li Yuanjin Yang Yuanjin Yang |
| author_sort | Yacheng Hu |
| collection | DOAJ |
| description | IntroductionOur understanding of the regulatory mechanisms that govern the development of sexual characteristics in sturgeon, particularly the Chinese sturgeon (Acipenser sinensis), is limited. Foxl2, a member of the forkhead/HNF-3-related transcription factor family, is known for its role in precise gene regulation within specific tissues and its control over ovarian aromatase, a key enzyme in ovarian development and maturation. This study aimed to investigate the sexually dimorphic expression of foxl2 during the molecular and morphological sex differentiation stages in Chinese sturgeon.MethodsWe successfully isolated the full-length cDNAs of foxl2 in Chinese sturgeon. We then examined the expression profiles of this gene across various somatic tissues in both female and male specimens.ResultsFoxl2 was predominantly expressed in the ovulatory organs of two-year-old Chinese sturgeon, with significantly lower expression in other somatic tissues. We observed sexual dimorphism in foxl2 expression within gonads that had achieved morphological differentiation.DiscussionThe results suggest that foxl2 expression is intricately linked to the process of ovarian differentiation in Chinese sturgeon. Furthermore, we propose that the mRNA quantities of foxl2 could serve as an accurate biomarker for sex determination in sturgeon during their early growth stages. |
| format | Article |
| id | doaj-art-e06faed08dbd4b4a96c8eba0b58b8ca2 |
| institution | OA Journals |
| issn | 2296-7745 |
| language | English |
| publishDate | 2024-12-01 |
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| series | Frontiers in Marine Science |
| spelling | doaj-art-e06faed08dbd4b4a96c8eba0b58b8ca22025-08-20T01:56:56ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452024-12-011110.3389/fmars.2024.15069321506932Molecular cloning, expression and functional analysis of foxl2 from Chinese sturgeon (Acipenser sinensis) in relation to sex differentiationYacheng Hu0Yacheng Hu1Ruihua Tan2Yang Li3Yang Li4Tingting Shu5Tingting Shu6Gang Yang7Zhong Chu8Haoran Wang9Xueqing Liu10Xueqing Liu11Xin Zhu12Xin Zhu13Binzhong Wang14Binzhong Wang15Baifu Guo16Baifu Guo17Runqiu Wang18Runqiu Wang19Huantao Qu20Huantao Qu21Haoyong Song22Haoyong Song23Dezhi Zhang24Dezhi Zhang25Hejun Du26Hejun Du27Zhiyuan Li28Zhiyuan Li29Jianbo Chang30Jianbo Chang31Dianchang Wang32Dan Xu33Wei Jiang34Wei Jiang35Qingfei Li36Yuanjin Yang37Yuanjin Yang38Chinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, ChinaHubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, ChinaNational Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai, ChinaChinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, ChinaHubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, ChinaChinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, ChinaHubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, ChinaSchool of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong, ChinaSchool of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong, ChinaSchool of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong, ChinaChinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, ChinaHubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, ChinaChinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, ChinaHubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, ChinaChinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, ChinaHubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, ChinaChinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, ChinaHubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, ChinaChinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, ChinaHubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, ChinaChinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, ChinaHubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, ChinaChinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, ChinaHubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, ChinaChinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, ChinaHubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, ChinaChinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, ChinaHubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, ChinaChinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, ChinaHubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, ChinaChinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, ChinaHubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, ChinaChina Three Gorges Corporation, Wuhan, Hubei, ChinaNational Pathogen Collection Center for Aquatic Animals, Shanghai Ocean University, Shanghai, ChinaChinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, ChinaHubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, ChinaSchool of Marine Science and Engineering, Qingdao Agricultural University, Qingdao, Shandong, ChinaChinese Sturgeon Research Institute, China Three Gorges Corporation, Yichang, Hubei, ChinaHubei Key Laboratory of Three Gorges Project for Conservation of Fishes, Yichang, Hubei, ChinaIntroductionOur understanding of the regulatory mechanisms that govern the development of sexual characteristics in sturgeon, particularly the Chinese sturgeon (Acipenser sinensis), is limited. Foxl2, a member of the forkhead/HNF-3-related transcription factor family, is known for its role in precise gene regulation within specific tissues and its control over ovarian aromatase, a key enzyme in ovarian development and maturation. This study aimed to investigate the sexually dimorphic expression of foxl2 during the molecular and morphological sex differentiation stages in Chinese sturgeon.MethodsWe successfully isolated the full-length cDNAs of foxl2 in Chinese sturgeon. We then examined the expression profiles of this gene across various somatic tissues in both female and male specimens.ResultsFoxl2 was predominantly expressed in the ovulatory organs of two-year-old Chinese sturgeon, with significantly lower expression in other somatic tissues. We observed sexual dimorphism in foxl2 expression within gonads that had achieved morphological differentiation.DiscussionThe results suggest that foxl2 expression is intricately linked to the process of ovarian differentiation in Chinese sturgeon. Furthermore, we propose that the mRNA quantities of foxl2 could serve as an accurate biomarker for sex determination in sturgeon during their early growth stages.https://www.frontiersin.org/articles/10.3389/fmars.2024.1506932/fullChinese sturgeonFOXL2sex differentiationgonadendangered fish |
| spellingShingle | Yacheng Hu Yacheng Hu Ruihua Tan Yang Li Yang Li Tingting Shu Tingting Shu Gang Yang Zhong Chu Haoran Wang Xueqing Liu Xueqing Liu Xin Zhu Xin Zhu Binzhong Wang Binzhong Wang Baifu Guo Baifu Guo Runqiu Wang Runqiu Wang Huantao Qu Huantao Qu Haoyong Song Haoyong Song Dezhi Zhang Dezhi Zhang Hejun Du Hejun Du Zhiyuan Li Zhiyuan Li Jianbo Chang Jianbo Chang Dianchang Wang Dan Xu Wei Jiang Wei Jiang Qingfei Li Yuanjin Yang Yuanjin Yang Molecular cloning, expression and functional analysis of foxl2 from Chinese sturgeon (Acipenser sinensis) in relation to sex differentiation Frontiers in Marine Science Chinese sturgeon FOXL2 sex differentiation gonad endangered fish |
| title | Molecular cloning, expression and functional analysis of foxl2 from Chinese sturgeon (Acipenser sinensis) in relation to sex differentiation |
| title_full | Molecular cloning, expression and functional analysis of foxl2 from Chinese sturgeon (Acipenser sinensis) in relation to sex differentiation |
| title_fullStr | Molecular cloning, expression and functional analysis of foxl2 from Chinese sturgeon (Acipenser sinensis) in relation to sex differentiation |
| title_full_unstemmed | Molecular cloning, expression and functional analysis of foxl2 from Chinese sturgeon (Acipenser sinensis) in relation to sex differentiation |
| title_short | Molecular cloning, expression and functional analysis of foxl2 from Chinese sturgeon (Acipenser sinensis) in relation to sex differentiation |
| title_sort | molecular cloning expression and functional analysis of foxl2 from chinese sturgeon acipenser sinensis in relation to sex differentiation |
| topic | Chinese sturgeon FOXL2 sex differentiation gonad endangered fish |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2024.1506932/full |
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