Three-dimensional diffractive acoustic tomography
Abstract Acoustically probing biological tissues with light or sound, photoacoustic and ultrasound imaging can provide anatomical, functional, and/or molecular information at depths far beyond the optical diffusion limit. However, most photoacoustic and ultrasound imaging systems rely on linear-arra...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2025-01-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-025-56435-3 |
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| author | Luca Menozzi Tri Vu Aidan J. Canning Harshal Rawtani Carlos Taboada Marie Elise Abi Antoun Chenshuo Ma Jesse Delia Van Tu Nguyen Soon-Woo Cho Jianing Chen Theresa Charity Yirui Xu Phuong Tran Jun Xia Gregory M. Palmer Tuan Vo-Dinh Liping Feng Junjie Yao |
| author_facet | Luca Menozzi Tri Vu Aidan J. Canning Harshal Rawtani Carlos Taboada Marie Elise Abi Antoun Chenshuo Ma Jesse Delia Van Tu Nguyen Soon-Woo Cho Jianing Chen Theresa Charity Yirui Xu Phuong Tran Jun Xia Gregory M. Palmer Tuan Vo-Dinh Liping Feng Junjie Yao |
| author_sort | Luca Menozzi |
| collection | DOAJ |
| description | Abstract Acoustically probing biological tissues with light or sound, photoacoustic and ultrasound imaging can provide anatomical, functional, and/or molecular information at depths far beyond the optical diffusion limit. However, most photoacoustic and ultrasound imaging systems rely on linear-array transducers with elevational focusing and are limited to two-dimensional imaging with anisotropic resolutions. Here, we present three-dimensional diffractive acoustic tomography (3D-DAT), which uses an off-the-shelf linear-array transducer with single-slit acoustic diffraction. Without jeopardizing its accessibility by general users, 3D-DAT has achieved simultaneous 3D photoacoustic and ultrasound imaging with optimal imaging performance in deep tissues, providing near-isotropic resolutions, high imaging speed, and a large field-of-view, as well as enhanced quantitative accuracy and detection sensitivity. Moreover, powered by the fast focal line volumetric reconstruction, 3D-DAT has achieved 50-fold faster reconstruction times than traditional photoacoustic imaging reconstruction. Using 3D-DAT on small animal models, we mapped the distribution of the biliverdin-binding serpin complex in glassfrogs, tracked gold nanoparticle accumulation in a mouse tumor model, imaged genetically-encoded photoswitchable tumors, and investigated polyfluoroalkyl substances exposure on developing embryos. With its enhanced imaging performance and high accessibility, 3D-DAT may find broad applications in fundamental life sciences and biomedical research. |
| format | Article |
| id | doaj-art-e05bd6051f4348ecb1b68f329cda7952 |
| institution | Kabale University |
| issn | 2041-1723 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj-art-e05bd6051f4348ecb1b68f329cda79522025-02-02T12:33:00ZengNature PortfolioNature Communications2041-17232025-01-0116111510.1038/s41467-025-56435-3Three-dimensional diffractive acoustic tomographyLuca Menozzi0Tri Vu1Aidan J. Canning2Harshal Rawtani3Carlos Taboada4Marie Elise Abi Antoun5Chenshuo Ma6Jesse Delia7Van Tu Nguyen8Soon-Woo Cho9Jianing Chen10Theresa Charity11Yirui Xu12Phuong Tran13Jun Xia14Gregory M. Palmer15Tuan Vo-Dinh16Liping Feng17Junjie Yao18Department of Biomedical Engineering, Duke UniversityDepartment of Biomedical Engineering, Duke UniversityDepartment of Biomedical Engineering, Duke UniversityDuke University School of MedicineDepartment of Biomedical Engineering, Duke UniversityTufts Medical Center, BostonDepartment of Biomedical Engineering, Duke UniversityAmerican Museum of Natural History, New York CityDepartment of Biomedical Engineering, Duke UniversityDepartment of Biomedical Engineering, Duke UniversityDepartment of Biomedical Engineering, Duke UniversityDepartment of Radiation Oncology, Duke University School of MedicineDepartment of Biomedical Engineering, Duke UniversityDepartment of Biomedical Engineering, Duke UniversityDepartment of Biomedical Engineering, University at Buffalo, BuffaloDepartment of Radiation Oncology, Duke University School of MedicineDepartment of Biomedical Engineering, Duke UniversityDuke University School of MedicineDepartment of Biomedical Engineering, Duke UniversityAbstract Acoustically probing biological tissues with light or sound, photoacoustic and ultrasound imaging can provide anatomical, functional, and/or molecular information at depths far beyond the optical diffusion limit. However, most photoacoustic and ultrasound imaging systems rely on linear-array transducers with elevational focusing and are limited to two-dimensional imaging with anisotropic resolutions. Here, we present three-dimensional diffractive acoustic tomography (3D-DAT), which uses an off-the-shelf linear-array transducer with single-slit acoustic diffraction. Without jeopardizing its accessibility by general users, 3D-DAT has achieved simultaneous 3D photoacoustic and ultrasound imaging with optimal imaging performance in deep tissues, providing near-isotropic resolutions, high imaging speed, and a large field-of-view, as well as enhanced quantitative accuracy and detection sensitivity. Moreover, powered by the fast focal line volumetric reconstruction, 3D-DAT has achieved 50-fold faster reconstruction times than traditional photoacoustic imaging reconstruction. Using 3D-DAT on small animal models, we mapped the distribution of the biliverdin-binding serpin complex in glassfrogs, tracked gold nanoparticle accumulation in a mouse tumor model, imaged genetically-encoded photoswitchable tumors, and investigated polyfluoroalkyl substances exposure on developing embryos. With its enhanced imaging performance and high accessibility, 3D-DAT may find broad applications in fundamental life sciences and biomedical research.https://doi.org/10.1038/s41467-025-56435-3 |
| spellingShingle | Luca Menozzi Tri Vu Aidan J. Canning Harshal Rawtani Carlos Taboada Marie Elise Abi Antoun Chenshuo Ma Jesse Delia Van Tu Nguyen Soon-Woo Cho Jianing Chen Theresa Charity Yirui Xu Phuong Tran Jun Xia Gregory M. Palmer Tuan Vo-Dinh Liping Feng Junjie Yao Three-dimensional diffractive acoustic tomography Nature Communications |
| title | Three-dimensional diffractive acoustic tomography |
| title_full | Three-dimensional diffractive acoustic tomography |
| title_fullStr | Three-dimensional diffractive acoustic tomography |
| title_full_unstemmed | Three-dimensional diffractive acoustic tomography |
| title_short | Three-dimensional diffractive acoustic tomography |
| title_sort | three dimensional diffractive acoustic tomography |
| url | https://doi.org/10.1038/s41467-025-56435-3 |
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