Transcriptional Profiling Reveals Kidney Neutrophil Heterogeneity in Both Healthy People and ccRCC Patients
Neutrophil is known to critically impact the development of renal diseases (e.g., the clear cell renal cell carcinoma (ccRCC)), whereas the heterogeneity of neutrophils in ccRCC remains unclear. In the present study, kidney biopsies from healthy donors and ccRCC tissues were collected for single-cel...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2021/5598627 |
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| author | Yiliang Meng Kai Cai Jingjie Zhao Keyu Huang Xiumei Ma Jian Song Yunguang Liu |
| author_facet | Yiliang Meng Kai Cai Jingjie Zhao Keyu Huang Xiumei Ma Jian Song Yunguang Liu |
| author_sort | Yiliang Meng |
| collection | DOAJ |
| description | Neutrophil is known to critically impact the development of renal diseases (e.g., the clear cell renal cell carcinoma (ccRCC)), whereas the heterogeneity of neutrophils in ccRCC remains unclear. In the present study, kidney biopsies from healthy donors and ccRCC tissues were collected for single-cell RNA sequencing (scRNA-seq). In addition, the subpopulations of neutrophils in a healthy kidney and in the tumor microenvironment (TME) of ccRCC were expressed and then analyzed. The genes reported previously were mapped to all subpopulations identified here. On that basis, biological theme comparison and Gene Set Enrichment Analysis (GSEA) were employed to reveal and compare relevant biological functions. In a healthy kidney, neutrophils exhibit two subpopulations: one is more associated with renal autoimmunity, probably acting as therapeutic target; the other is suggested to resist infectious microorganisms. It is noteworthy that six subpopulations were identified in ccRCC biopsy, and two were more relevant to autoimmunity, while the other four are more relevant to the tumor pathology. Besides, ccRCC neutrophil could resist anticancer immune therapies of ipilimumab and pembrolizumab for their low/no expressions of CTLA-4, PD-1, and PD-L1. Thus, this study can help understand the heterogeneity and pathological significance of neutrophils in renal diseases. |
| format | Article |
| id | doaj-art-e05128ba7fb24ab5ae0ea5e4a65c7015 |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-e05128ba7fb24ab5ae0ea5e4a65c70152025-02-03T01:10:10ZengWileyJournal of Immunology Research2314-88612314-71562021-01-01202110.1155/2021/55986275598627Transcriptional Profiling Reveals Kidney Neutrophil Heterogeneity in Both Healthy People and ccRCC PatientsYiliang Meng0Kai Cai1Jingjie Zhao2Keyu Huang3Xiumei Ma4Jian Song5Yunguang Liu6Department of Oncology, People’s Hospital of Baise, Baise City, Guangxi Province, ChinaRadiation Therapy Center, The First Affiliated Hospital of Guangxi University of Chinese Medicine, Guangxi Province, ChinaLife Science and Clinical Research Center, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaDepartment of Hepatobiliary Surgery, The First Affiliated Hospital of Guangxi Medical University, Nanning City, Guangxi Province, ChinaDepartment of Radiation Oncology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Radiation Oncology, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, ChinaDepartment of Pediatrics, The Affiliated Hospital of Youjiang Medical University for Nationalities, Baise City, Guangxi Province, ChinaNeutrophil is known to critically impact the development of renal diseases (e.g., the clear cell renal cell carcinoma (ccRCC)), whereas the heterogeneity of neutrophils in ccRCC remains unclear. In the present study, kidney biopsies from healthy donors and ccRCC tissues were collected for single-cell RNA sequencing (scRNA-seq). In addition, the subpopulations of neutrophils in a healthy kidney and in the tumor microenvironment (TME) of ccRCC were expressed and then analyzed. The genes reported previously were mapped to all subpopulations identified here. On that basis, biological theme comparison and Gene Set Enrichment Analysis (GSEA) were employed to reveal and compare relevant biological functions. In a healthy kidney, neutrophils exhibit two subpopulations: one is more associated with renal autoimmunity, probably acting as therapeutic target; the other is suggested to resist infectious microorganisms. It is noteworthy that six subpopulations were identified in ccRCC biopsy, and two were more relevant to autoimmunity, while the other four are more relevant to the tumor pathology. Besides, ccRCC neutrophil could resist anticancer immune therapies of ipilimumab and pembrolizumab for their low/no expressions of CTLA-4, PD-1, and PD-L1. Thus, this study can help understand the heterogeneity and pathological significance of neutrophils in renal diseases.http://dx.doi.org/10.1155/2021/5598627 |
| spellingShingle | Yiliang Meng Kai Cai Jingjie Zhao Keyu Huang Xiumei Ma Jian Song Yunguang Liu Transcriptional Profiling Reveals Kidney Neutrophil Heterogeneity in Both Healthy People and ccRCC Patients Journal of Immunology Research |
| title | Transcriptional Profiling Reveals Kidney Neutrophil Heterogeneity in Both Healthy People and ccRCC Patients |
| title_full | Transcriptional Profiling Reveals Kidney Neutrophil Heterogeneity in Both Healthy People and ccRCC Patients |
| title_fullStr | Transcriptional Profiling Reveals Kidney Neutrophil Heterogeneity in Both Healthy People and ccRCC Patients |
| title_full_unstemmed | Transcriptional Profiling Reveals Kidney Neutrophil Heterogeneity in Both Healthy People and ccRCC Patients |
| title_short | Transcriptional Profiling Reveals Kidney Neutrophil Heterogeneity in Both Healthy People and ccRCC Patients |
| title_sort | transcriptional profiling reveals kidney neutrophil heterogeneity in both healthy people and ccrcc patients |
| url | http://dx.doi.org/10.1155/2021/5598627 |
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