Nano-haemoglobin-based oxygen carrier increases the radiosensitivity of non-small-cell lung cancer
Haemoglobin-based oxygen carriers (HBOCs) could improve the hypoxic state of non-small-cell lung cancer (NSCLC) and increase radiotherapy sensitivity. We assessed the in vitro effects of nano-HBOC + irradiation therapy (IR) on NSCLC cells and the in vivo effect on a mouse model. H385 human NSCLC cel...
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Taylor & Francis Group
2025-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2025.2503369 |
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| author | Changmin Liu Yong Li Shanhui Feng Xiaoran Lv Fengjuan Li Binglou Wong Jiaxin Liu Chengmin Yang |
| author_facet | Changmin Liu Yong Li Shanhui Feng Xiaoran Lv Fengjuan Li Binglou Wong Jiaxin Liu Chengmin Yang |
| author_sort | Changmin Liu |
| collection | DOAJ |
| description | Haemoglobin-based oxygen carriers (HBOCs) could improve the hypoxic state of non-small-cell lung cancer (NSCLC) and increase radiotherapy sensitivity. We assessed the in vitro effects of nano-HBOC + irradiation therapy (IR) on NSCLC cells and the in vivo effect on a mouse model. H385 human NSCLC cell line was used to evaluate the nano-HBOC effect + IR on the cellular partial pressure of oxygen (pO2), cell activity and changes in reactive oxygen species (ROS) 1–2 h post-exposure. An NSCLC tumour-bearing mouse model was established to evaluate nano-HBOC+IR efficacy 28 d post-exposure. In vitro, pO2 tended to increase in nano-HBOC groups versus control, cell activity decreased (p < 0.01) and ROS level increased (p < 0.05). Post-irradiation, pO2 increased in nano-HBOC+IR groups versus control (p < 0.01), viability decreased (p < 0.01) and ROS increased (p < 0.01). No significant difference between nano-HBOC groups was observed. In vivo, nano-HBOC was most abundant at the tumour site and pO2 increased 6 h post-injection (p > 0.05). Tumour size was smaller in the IR and nano-HBOC+IR groups versus control. ROS levels and cell death were significantly increased. Nano-HBOC can improve pO2, enhance radiotherapy’s inhibitory ability on NSCLC cell lines and tumour-bearing mouse models, and promote ROS release. |
| format | Article |
| id | doaj-art-e045e13fb3c849ad8b76c22e9237b31e |
| institution | Kabale University |
| issn | 2169-1401 2169-141X |
| language | English |
| publishDate | 2025-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj-art-e045e13fb3c849ad8b76c22e9237b31e2025-08-20T03:55:23ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2025-12-0153124425210.1080/21691401.2025.2503369Nano-haemoglobin-based oxygen carrier increases the radiosensitivity of non-small-cell lung cancerChangmin Liu0Yong Li1Shanhui Feng2Xiaoran Lv3Fengjuan Li4Binglou Wong5Jiaxin Liu6Chengmin Yang7Institute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College Institute of Blood Transfusion, Chengdu, ChinaInterventional Therapy Department, Tianjin Key Laboratory of Cancer Prevention and Therapy, Tianjin Medical University Cancer Institute and Hospital, Tianjin, ChinaDepartment of Oncology, Binzhou Medical University Hospital, Binzhou, ChinaDepartment of Oncology, Binzhou Medical University Hospital, Binzhou, ChinaSchool of Pharmaceutical Sciences, Tiangong University, Tianjin, ChinaInstitute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College Institute of Blood Transfusion, Chengdu, ChinaInstitute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College Institute of Blood Transfusion, Chengdu, ChinaInstitute of Blood Transfusion, Chinese Academy of Medical Sciences and Peking Union Medical College Institute of Blood Transfusion, Chengdu, ChinaHaemoglobin-based oxygen carriers (HBOCs) could improve the hypoxic state of non-small-cell lung cancer (NSCLC) and increase radiotherapy sensitivity. We assessed the in vitro effects of nano-HBOC + irradiation therapy (IR) on NSCLC cells and the in vivo effect on a mouse model. H385 human NSCLC cell line was used to evaluate the nano-HBOC effect + IR on the cellular partial pressure of oxygen (pO2), cell activity and changes in reactive oxygen species (ROS) 1–2 h post-exposure. An NSCLC tumour-bearing mouse model was established to evaluate nano-HBOC+IR efficacy 28 d post-exposure. In vitro, pO2 tended to increase in nano-HBOC groups versus control, cell activity decreased (p < 0.01) and ROS level increased (p < 0.05). Post-irradiation, pO2 increased in nano-HBOC+IR groups versus control (p < 0.01), viability decreased (p < 0.01) and ROS increased (p < 0.01). No significant difference between nano-HBOC groups was observed. In vivo, nano-HBOC was most abundant at the tumour site and pO2 increased 6 h post-injection (p > 0.05). Tumour size was smaller in the IR and nano-HBOC+IR groups versus control. ROS levels and cell death were significantly increased. Nano-HBOC can improve pO2, enhance radiotherapy’s inhibitory ability on NSCLC cell lines and tumour-bearing mouse models, and promote ROS release.https://www.tandfonline.com/doi/10.1080/21691401.2025.2503369Nano-haemoglobin-based oxygen carrierradiotherapynon-small-cell lung cancerreactive oxygen species |
| spellingShingle | Changmin Liu Yong Li Shanhui Feng Xiaoran Lv Fengjuan Li Binglou Wong Jiaxin Liu Chengmin Yang Nano-haemoglobin-based oxygen carrier increases the radiosensitivity of non-small-cell lung cancer Artificial Cells, Nanomedicine, and Biotechnology Nano-haemoglobin-based oxygen carrier radiotherapy non-small-cell lung cancer reactive oxygen species |
| title | Nano-haemoglobin-based oxygen carrier increases the radiosensitivity of non-small-cell lung cancer |
| title_full | Nano-haemoglobin-based oxygen carrier increases the radiosensitivity of non-small-cell lung cancer |
| title_fullStr | Nano-haemoglobin-based oxygen carrier increases the radiosensitivity of non-small-cell lung cancer |
| title_full_unstemmed | Nano-haemoglobin-based oxygen carrier increases the radiosensitivity of non-small-cell lung cancer |
| title_short | Nano-haemoglobin-based oxygen carrier increases the radiosensitivity of non-small-cell lung cancer |
| title_sort | nano haemoglobin based oxygen carrier increases the radiosensitivity of non small cell lung cancer |
| topic | Nano-haemoglobin-based oxygen carrier radiotherapy non-small-cell lung cancer reactive oxygen species |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2025.2503369 |
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