Development and validation of a droplet digital PCR assay for sensitive detection and quantification of Phytophthora nicotianae

Development and validation of a droplet digital PCR assay for sensitive detection and quantification of Phytophthora nicotianae

Tobacco black shank (TBS) disease, caused by Phytophthora nicotianae (P. nicotianae), poses a significant threat to global agriculture and results in substantial economic losses. Traditional methods, like culture-based techniques and quantitative polymerase chain reaction (qPCR), aid pathogen identi...

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Bibliographic Details
Main Authors: Yuanyuan Liu, Jiali Li, Zining Guo, Chao Feng, Yunhua Gao, Danmei Liu, Di Wang
Format: Article
Language:English
Published: Frontiers Media S.A. 2025-04-01
Series:Frontiers in Plant Science
Subjects:
Phytophthora nicotianae
droplet digital PCR
quantitative PCR
pathogen detection
plant disease management
Online Access:https://www.frontiersin.org/articles/10.3389/fpls.2025.1573949/full
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