Detection of Gallibacterium anatis in layers by PCR and their histopathological observation in natural case
In order to observe the histopathological changes of the layer oviduct cysts infected with Gallibacterium anatis, target genes were amplified by PCR from genome DNA extracted from lung, trachea, oviduct and ovary of nine birds infected naturally, using genus-specificity primes of Gallibacterium anat...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Zhejiang University Press
2010-01-01
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| Series: | 浙江大学学报. 农业与生命科学版 |
| Subjects: | |
| Online Access: | https://www.academax.com/doi/10.3785/j.issn.1008-9209.2010.01.018 |
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| Summary: | In order to observe the histopathological changes of the layer oviduct cysts infected with Gallibacterium anatis, target genes were amplified by PCR from genome DNA extracted from lung, trachea, oviduct and ovary of nine birds infected naturally, using genus-specificity primes of Gallibacterium anatis, and the Gallibacterium anatis PCR positive samples were fixed in the formalin and dehydrated with gradient alcohol. The paraffin sections were made and stained with haematoxyylin and eosin, then were observed by light microscope. The results showed that Gallibacterium anatis mainly induced exfoliation of the mucosa epithelium cells, hyperaemia of cortex capillary and infiltration of inflammation cells in up-respiratory tract, as well as inflammation cells in mucosa of magnum and uterus in oviduct and nucleus of secretory cells broken into small pieces. This pathological changes could be one of the important diagnosis points of the disease, which laid the foundation for elucidating the characteristic pathological changes of oviduct and the development mechanism of the disease. |
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| ISSN: | 1008-9209 2097-5155 |