The Method of Segmentation of Cervical Nuclei in Complex Background

Automatic screening technology developed in recent years. It applies image processing, and first recognizes nucleus and then measures the DNA contents accurately, so it can provide auxiliary for a doctor′s diagnosis. Image segmentation is the key technique of automatic screening system which directl...

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Main Authors: ZHAO Jing, LIANG Long-kai, HE Yong-jun, XIE Yi-ning
Format: Article
Language:zho
Published: Harbin University of Science and Technology Publications 2019-06-01
Series:Journal of Harbin University of Science and Technology
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Online Access:https://hlgxb.hrbust.edu.cn/#/digest?ArticleID=1677
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author ZHAO Jing
LIANG Long-kai
HE Yong-jun
XIE Yi-ning
author_facet ZHAO Jing
LIANG Long-kai
HE Yong-jun
XIE Yi-ning
author_sort ZHAO Jing
collection DOAJ
description Automatic screening technology developed in recent years. It applies image processing, and first recognizes nucleus and then measures the DNA contents accurately, so it can provide auxiliary for a doctor′s diagnosis. Image segmentation is the key technique of automatic screening system which directly determines the performance of the systems. However, the imaging environments under the microscope are complex. One the one hand, uneven illumination, background shading and uneven dyed nucleus exist. On the other hand, there are inevitably blood cells, lymphocytes, garbage, impurities and conglobation cells in cell images. These conditions degrade the performance of image segmentation. In order to solve these problems, we put forward a method to segment cervical nuclei in complex background. This method first employs the local threshold method to segment images. In this procedure we propose a parameter adapting method which adjusts its parameters automatically according to the function of local threshold window size and the binarized outline number. The local threshold method transforms an image into a binary image which is then passed to image corrosion operator to generate a marking image. With the binary image, the watershed algorithm was finally performed to segment the image. The experiment shows that the method can adapt to the complex image environment and separate the cells with lower overlapping nuclei images.
format Article
id doaj-art-e01a17fe302a461b82b4e511caed13d8
institution Kabale University
issn 1007-2683
language zho
publishDate 2019-06-01
publisher Harbin University of Science and Technology Publications
record_format Article
series Journal of Harbin University of Science and Technology
spelling doaj-art-e01a17fe302a461b82b4e511caed13d82025-08-20T03:43:43ZzhoHarbin University of Science and Technology PublicationsJournal of Harbin University of Science and Technology1007-26832019-06-012403222810.15938/j.jhust.2019.03.004The Method of Segmentation of Cervical Nuclei in Complex BackgroundZHAO Jing0LIANG Long-kai1HE Yong-jun2XIE Yi-ning3Harbin University of Science and Technology, School of Computer Science and Technology, Harbin 150080, ChinaHarbin University of Science and Technology, School of Computer Science and Technology, Harbin 150080, ChinaHarbin University of Science and Technology, School of Computer Science and Technology, Harbin 150080, ChinaHarbin University of Science and Technology, School of Computer Science and Technology, Harbin 150080, ChinaAutomatic screening technology developed in recent years. It applies image processing, and first recognizes nucleus and then measures the DNA contents accurately, so it can provide auxiliary for a doctor′s diagnosis. Image segmentation is the key technique of automatic screening system which directly determines the performance of the systems. However, the imaging environments under the microscope are complex. One the one hand, uneven illumination, background shading and uneven dyed nucleus exist. On the other hand, there are inevitably blood cells, lymphocytes, garbage, impurities and conglobation cells in cell images. These conditions degrade the performance of image segmentation. In order to solve these problems, we put forward a method to segment cervical nuclei in complex background. This method first employs the local threshold method to segment images. In this procedure we propose a parameter adapting method which adjusts its parameters automatically according to the function of local threshold window size and the binarized outline number. The local threshold method transforms an image into a binary image which is then passed to image corrosion operator to generate a marking image. With the binary image, the watershed algorithm was finally performed to segment the image. The experiment shows that the method can adapt to the complex image environment and separate the cells with lower overlapping nuclei images.https://hlgxb.hrbust.edu.cn/#/digest?ArticleID=1677nucleus segmentationparameter adaptivewatershed algorithmlocal threshold method
spellingShingle ZHAO Jing
LIANG Long-kai
HE Yong-jun
XIE Yi-ning
The Method of Segmentation of Cervical Nuclei in Complex Background
Journal of Harbin University of Science and Technology
nucleus segmentation
parameter adaptive
watershed algorithm
local threshold method
title The Method of Segmentation of Cervical Nuclei in Complex Background
title_full The Method of Segmentation of Cervical Nuclei in Complex Background
title_fullStr The Method of Segmentation of Cervical Nuclei in Complex Background
title_full_unstemmed The Method of Segmentation of Cervical Nuclei in Complex Background
title_short The Method of Segmentation of Cervical Nuclei in Complex Background
title_sort method of segmentation of cervical nuclei in complex background
topic nucleus segmentation
parameter adaptive
watershed algorithm
local threshold method
url https://hlgxb.hrbust.edu.cn/#/digest?ArticleID=1677
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