Increased TNF-α Initiates Cytoplasmic Vacuolization in Whole Blood Coculture with Dengue Virus
During the acute febrile phase of dengue virus (DENV) infection, viremia can cause severe systemic immune responses accompanied by hematologic disorders. This study investigated the potential induction and mechanism of the cytopathic effects of DENV on peripheral blood cells ex vivo. At one day post...
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| Format: | Article |
| Language: | English |
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Wiley
2021-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2021/6654617 |
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| author | Rahmat Dani Satria Tzu-Wen Huang Ming-Kai Jhan Ting-Jing Shen Po-Chun Tseng Yun-Ting Wang Zhen-Yu Yang Chung-Hsi Hsing Chiou-Feng Lin |
| author_facet | Rahmat Dani Satria Tzu-Wen Huang Ming-Kai Jhan Ting-Jing Shen Po-Chun Tseng Yun-Ting Wang Zhen-Yu Yang Chung-Hsi Hsing Chiou-Feng Lin |
| author_sort | Rahmat Dani Satria |
| collection | DOAJ |
| description | During the acute febrile phase of dengue virus (DENV) infection, viremia can cause severe systemic immune responses accompanied by hematologic disorders. This study investigated the potential induction and mechanism of the cytopathic effects of DENV on peripheral blood cells ex vivo. At one day postinfection, there was viral nonstructural protein NS1 but no further virus replication measured in the whole blood culture. Notably, DENV exposure caused significant vacuolization in monocytic phagocytes. With a minor change in the complete blood cell count, except for a minor increase in neutrophils and a significant decrease in monocytes, the immune profiling assay identified several changes, particularly a significant reduction in CD14-positive monocytes as well as CD11c-positive dendritic cells. Abnormal production of TNF-α was highly associated with the induction of vacuolization. Manipulating TNF-α expression resulted in cytopathogenic effects. These results demonstrate the potential hematological damage caused by ex vivo DENV-induced TNF-α. |
| format | Article |
| id | doaj-art-e0158f08724b48e294d1db83905469ec |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2021-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-e0158f08724b48e294d1db83905469ec2025-02-03T06:05:36ZengWileyJournal of Immunology Research2314-88612314-71562021-01-01202110.1155/2021/66546176654617Increased TNF-α Initiates Cytoplasmic Vacuolization in Whole Blood Coculture with Dengue VirusRahmat Dani Satria0Tzu-Wen Huang1Ming-Kai Jhan2Ting-Jing Shen3Po-Chun Tseng4Yun-Ting Wang5Zhen-Yu Yang6Chung-Hsi Hsing7Chiou-Feng Lin8International Ph.D. Program in Medicine, College of Medicine, Taipei Medical University, Taipei 110, TaiwanDepartment of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, TaiwanDepartment of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, TaiwanDepartment of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, TaiwanDepartment of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, TaiwanDepartment of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, TaiwanDepartment of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, TaiwanDepartment of Medical Research, Chi Mei Medical Center, Tainan 710, TaiwanDepartment of Microbiology and Immunology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 110, TaiwanDuring the acute febrile phase of dengue virus (DENV) infection, viremia can cause severe systemic immune responses accompanied by hematologic disorders. This study investigated the potential induction and mechanism of the cytopathic effects of DENV on peripheral blood cells ex vivo. At one day postinfection, there was viral nonstructural protein NS1 but no further virus replication measured in the whole blood culture. Notably, DENV exposure caused significant vacuolization in monocytic phagocytes. With a minor change in the complete blood cell count, except for a minor increase in neutrophils and a significant decrease in monocytes, the immune profiling assay identified several changes, particularly a significant reduction in CD14-positive monocytes as well as CD11c-positive dendritic cells. Abnormal production of TNF-α was highly associated with the induction of vacuolization. Manipulating TNF-α expression resulted in cytopathogenic effects. These results demonstrate the potential hematological damage caused by ex vivo DENV-induced TNF-α.http://dx.doi.org/10.1155/2021/6654617 |
| spellingShingle | Rahmat Dani Satria Tzu-Wen Huang Ming-Kai Jhan Ting-Jing Shen Po-Chun Tseng Yun-Ting Wang Zhen-Yu Yang Chung-Hsi Hsing Chiou-Feng Lin Increased TNF-α Initiates Cytoplasmic Vacuolization in Whole Blood Coculture with Dengue Virus Journal of Immunology Research |
| title | Increased TNF-α Initiates Cytoplasmic Vacuolization in Whole Blood Coculture with Dengue Virus |
| title_full | Increased TNF-α Initiates Cytoplasmic Vacuolization in Whole Blood Coculture with Dengue Virus |
| title_fullStr | Increased TNF-α Initiates Cytoplasmic Vacuolization in Whole Blood Coculture with Dengue Virus |
| title_full_unstemmed | Increased TNF-α Initiates Cytoplasmic Vacuolization in Whole Blood Coculture with Dengue Virus |
| title_short | Increased TNF-α Initiates Cytoplasmic Vacuolization in Whole Blood Coculture with Dengue Virus |
| title_sort | increased tnf α initiates cytoplasmic vacuolization in whole blood coculture with dengue virus |
| url | http://dx.doi.org/10.1155/2021/6654617 |
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