NMR metabolic profiling of the liver following administrationof alcohol andthemushroom Ganoderma lucidum in rats
We have evaluated the efficiency of a metabonomic approach to metabolic phenotyping and detection of early metabolic changes under a toxic influence. For this purpose, a metabolic profiling of rat liver was performed with 1H NMR spectroscopy. Rat tissues from animals in three groups were analyzed. G...
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2015-12-01
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| Series: | Вавиловский журнал генетики и селекции |
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| Online Access: | https://vavilov.elpub.ru/jour/article/view/442 |
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| author | M. S. Krestina O. B. Shevelev I. V. Koptyug L. A. Gerlinskaya S. E. Peltek A. E. Akulov |
| author_facet | M. S. Krestina O. B. Shevelev I. V. Koptyug L. A. Gerlinskaya S. E. Peltek A. E. Akulov |
| author_sort | M. S. Krestina |
| collection | DOAJ |
| description | We have evaluated the efficiency of a metabonomic approach to metabolic phenotyping and detection of early metabolic changes under a toxic influence. For this purpose, a metabolic profiling of rat liver was performed with 1H NMR spectroscopy. Rat tissues from animals in three groups were analyzed. Group C consisted of control animals; animals in group A received alcohol repeatedly (15 % ethanol); and animals in group A+ R received alcohol in combination with a hepatoprotective herbal medicine (Reishi, Ganoderma lucidum) repeatedly. Noteworthy, alcohol consumption did not cause pathological changes, but stimulated hepatocyte proliferation. Our data suggest that changes in metabolite concentrations in A represent a typical metabolic response to alcohol consumption, namely decrease in glycine, leucine, isoleucine, valine, choline and lactate content, and increase in TMAO content. Treatment with Reishi (A+ R) had positive effects, in that it restored the levels of glycine, valine and TMAO. Furthermore, increase in NAD, ATP, UTP, succinate, pyranose, and acetate concentrations was observed in A+ R. A correlation was found between the valine, isoleucine, lactate, choline, and pyranose content and the number of binuclear hepatocytes. Binuclear hepatocytes indicate proliferative activity, and the concentration of the metabolites participating in the formation of new hepatic cells decreases. Thus, the study of liver tissues by 1H NMR spectroscopy allows for detection of early changes in metabolite concentrations following chronic consumption of alcohol at insignificant doses. Consequently, 1H NMR spectroscopy can serve as a promising approach to detecting alcohol-related liver pathologies and assessing the efficiency of the therapy used. |
| format | Article |
| id | doaj-art-e014cf8e911f4172b332b57f1dfff614 |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2015-12-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-e014cf8e911f4172b332b57f1dfff6142025-02-01T09:58:02ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592015-12-0119449950410.18699/VJ15.067400NMR metabolic profiling of the liver following administrationof alcohol andthemushroom Ganoderma lucidum in ratsM. S. Krestina0O. B. Shevelev1I. V. Koptyug2L. A. Gerlinskaya3S. E. Peltek4A. E. Akulov5International Tomography Center of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, Russia Novosibirsk State University, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInternational Tomography Center of the Siberian Branch of the Russian Academy of Sciences, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaInstitute of Cytology and Genetics SB RAS, Novosibirsk, RussiaWe have evaluated the efficiency of a metabonomic approach to metabolic phenotyping and detection of early metabolic changes under a toxic influence. For this purpose, a metabolic profiling of rat liver was performed with 1H NMR spectroscopy. Rat tissues from animals in three groups were analyzed. Group C consisted of control animals; animals in group A received alcohol repeatedly (15 % ethanol); and animals in group A+ R received alcohol in combination with a hepatoprotective herbal medicine (Reishi, Ganoderma lucidum) repeatedly. Noteworthy, alcohol consumption did not cause pathological changes, but stimulated hepatocyte proliferation. Our data suggest that changes in metabolite concentrations in A represent a typical metabolic response to alcohol consumption, namely decrease in glycine, leucine, isoleucine, valine, choline and lactate content, and increase in TMAO content. Treatment with Reishi (A+ R) had positive effects, in that it restored the levels of glycine, valine and TMAO. Furthermore, increase in NAD, ATP, UTP, succinate, pyranose, and acetate concentrations was observed in A+ R. A correlation was found between the valine, isoleucine, lactate, choline, and pyranose content and the number of binuclear hepatocytes. Binuclear hepatocytes indicate proliferative activity, and the concentration of the metabolites participating in the formation of new hepatic cells decreases. Thus, the study of liver tissues by 1H NMR spectroscopy allows for detection of early changes in metabolite concentrations following chronic consumption of alcohol at insignificant doses. Consequently, 1H NMR spectroscopy can serve as a promising approach to detecting alcohol-related liver pathologies and assessing the efficiency of the therapy used.https://vavilov.elpub.ru/jour/article/view/442sprague-dawley ratsliveralcoholganoderma lucidum1h nmr spectroscopyin vitrometabonomicsdiagnosis of early metabolic disorders |
| spellingShingle | M. S. Krestina O. B. Shevelev I. V. Koptyug L. A. Gerlinskaya S. E. Peltek A. E. Akulov NMR metabolic profiling of the liver following administrationof alcohol andthemushroom Ganoderma lucidum in rats Вавиловский журнал генетики и селекции sprague-dawley rats liver alcohol ganoderma lucidum 1h nmr spectroscopy in vitro metabonomics diagnosis of early metabolic disorders |
| title | NMR metabolic profiling of the liver following administrationof alcohol andthemushroom Ganoderma lucidum in rats |
| title_full | NMR metabolic profiling of the liver following administrationof alcohol andthemushroom Ganoderma lucidum in rats |
| title_fullStr | NMR metabolic profiling of the liver following administrationof alcohol andthemushroom Ganoderma lucidum in rats |
| title_full_unstemmed | NMR metabolic profiling of the liver following administrationof alcohol andthemushroom Ganoderma lucidum in rats |
| title_short | NMR metabolic profiling of the liver following administrationof alcohol andthemushroom Ganoderma lucidum in rats |
| title_sort | nmr metabolic profiling of the liver following administrationof alcohol andthemushroom ganoderma lucidum in rats |
| topic | sprague-dawley rats liver alcohol ganoderma lucidum 1h nmr spectroscopy in vitro metabonomics diagnosis of early metabolic disorders |
| url | https://vavilov.elpub.ru/jour/article/view/442 |
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