Identification of Specific Cell Surface Markers on Immune Cells of Squirrel Monkeys (Saimiri sciureus)
Nonhuman primates are an important experimental model for the development of targeted biological therapeutics because of their immunological closeness to humans. However, there are very few antibody reagents relevant for delineating the different immune cell subsets based on nonhuman primate antigen...
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| Format: | Article |
| Language: | English |
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Wiley
2024-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2024/8215195 |
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| author | Bharti P. Nehete Ashley DeLise Pramod N. Nehete |
| author_facet | Bharti P. Nehete Ashley DeLise Pramod N. Nehete |
| author_sort | Bharti P. Nehete |
| collection | DOAJ |
| description | Nonhuman primates are an important experimental model for the development of targeted biological therapeutics because of their immunological closeness to humans. However, there are very few antibody reagents relevant for delineating the different immune cell subsets based on nonhuman primate antigens directly or with cross-reactivity to those in humans. Here, we report specific expression of HLA-DR, PD-1, and CD123 on different circulating immune cell subsets in the peripheral blood that included T cells (CD3+), T cells subsets (CD4+ and CD8+), B cells (CD20+), natural killer (NK) cells (CD3–CD16+), and natural killer T cells (CD3+CD16+) along with different monocyte subsets in squirrel monkey (Saimiri sciureus). We established cross-reactivity of commercial mouse antihuman monoclonal antibodies (mAbs), with these various immune cell surface markers. These findings should aid further future comprehensive understanding of the immune parameters and identification of new biomarkers to significantly improve SQM as a model for biomedical studies. |
| format | Article |
| id | doaj-art-e00e4ae2fcb64fadbbb2c4aaddfb4dc1 |
| institution | Kabale University |
| issn | 2314-7156 |
| language | English |
| publishDate | 2024-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-e00e4ae2fcb64fadbbb2c4aaddfb4dc12025-02-03T01:29:51ZengWileyJournal of Immunology Research2314-71562024-01-01202410.1155/2024/8215195Identification of Specific Cell Surface Markers on Immune Cells of Squirrel Monkeys (Saimiri sciureus)Bharti P. Nehete0Ashley DeLise1Pramod N. Nehete2Department of Comparative MedicineDepartment of Comparative MedicineDepartment of Comparative MedicineNonhuman primates are an important experimental model for the development of targeted biological therapeutics because of their immunological closeness to humans. However, there are very few antibody reagents relevant for delineating the different immune cell subsets based on nonhuman primate antigens directly or with cross-reactivity to those in humans. Here, we report specific expression of HLA-DR, PD-1, and CD123 on different circulating immune cell subsets in the peripheral blood that included T cells (CD3+), T cells subsets (CD4+ and CD8+), B cells (CD20+), natural killer (NK) cells (CD3–CD16+), and natural killer T cells (CD3+CD16+) along with different monocyte subsets in squirrel monkey (Saimiri sciureus). We established cross-reactivity of commercial mouse antihuman monoclonal antibodies (mAbs), with these various immune cell surface markers. These findings should aid further future comprehensive understanding of the immune parameters and identification of new biomarkers to significantly improve SQM as a model for biomedical studies.http://dx.doi.org/10.1155/2024/8215195 |
| spellingShingle | Bharti P. Nehete Ashley DeLise Pramod N. Nehete Identification of Specific Cell Surface Markers on Immune Cells of Squirrel Monkeys (Saimiri sciureus) Journal of Immunology Research |
| title | Identification of Specific Cell Surface Markers on Immune Cells of Squirrel Monkeys (Saimiri sciureus) |
| title_full | Identification of Specific Cell Surface Markers on Immune Cells of Squirrel Monkeys (Saimiri sciureus) |
| title_fullStr | Identification of Specific Cell Surface Markers on Immune Cells of Squirrel Monkeys (Saimiri sciureus) |
| title_full_unstemmed | Identification of Specific Cell Surface Markers on Immune Cells of Squirrel Monkeys (Saimiri sciureus) |
| title_short | Identification of Specific Cell Surface Markers on Immune Cells of Squirrel Monkeys (Saimiri sciureus) |
| title_sort | identification of specific cell surface markers on immune cells of squirrel monkeys saimiri sciureus |
| url | http://dx.doi.org/10.1155/2024/8215195 |
| work_keys_str_mv | AT bhartipnehete identificationofspecificcellsurfacemarkersonimmunecellsofsquirrelmonkeyssaimirisciureus AT ashleydelise identificationofspecificcellsurfacemarkersonimmunecellsofsquirrelmonkeyssaimirisciureus AT pramodnnehete identificationofspecificcellsurfacemarkersonimmunecellsofsquirrelmonkeyssaimirisciureus |