INVESTIGATE THE ANTIBACTERIAL ACTIVITY OF AMYGDALIN AGAINST SOME TYPES OF ANTIBIOTIC RESISTANT PATHOGENIC BACTERIA
Extraction of Amygdalin from apple seeds by alcoholic extraction procedure were doneand applied a high-performance liquid chromatographic (HPLC) procedure for quantification ofit to investigate extraction efficiency and (16) bacterial isolates ( 4 isolates of Staphylococcusaureus, 4 isolates of Esch...
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College of Veterinary Medicine, University of Basrah, Iraq
2018-11-01
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| Series: | Basrah Journal of Veterinary Research |
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| Online Access: | https://bjvr.uobasrah.edu.iq/article_174007_7c6b216c4af650205a5132cf1e6c5ea6.pdf |
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| author | Ahmed A. Mhawesh Dhafar N. Al-Ugaili Yasameen Mad olaiwy |
| author_facet | Ahmed A. Mhawesh Dhafar N. Al-Ugaili Yasameen Mad olaiwy |
| author_sort | Ahmed A. Mhawesh |
| collection | DOAJ |
| description | Extraction of Amygdalin from apple seeds by alcoholic extraction procedure were doneand applied a high-performance liquid chromatographic (HPLC) procedure for quantification ofit to investigate extraction efficiency and (16) bacterial isolates ( 4 isolates of Staphylococcusaureus, 4 isolates of Escherichia coli , 4 isolates of Pseudomonas aeruginosa and 4 isolates ofStreptococcus pyogenes from skin infections samples are choose by accomplished of drugsensitivity tests as a multidrug resistant, the antimicrobial activity of methanolic extract ofAmygdalin from crushed apple seeds was tested against each group of four pathogenic bacteriaspecies of gram positive (Staphylococcus aureus and Streptococcus pyogenes ) and gramnegative (Escherichia coli and Pseudomonas aeruginosa) by well diffusion method, the resultsshown that plant extract gave antibacterial activity through the inhibition zone , but theconcentration of the raw material was more inhibited than the dilute substance concentration,inhibition of the substance was obtained on all types of bacteria were in Staphylococcus aureus,inhibition zone of substances concentrations (100 mg/ml, 50 mg/ml) were (40, 28 mm)respectively , in Pseudomonas aeruginosa inhibition zone of substances concentrations (100mg/ml, 50 mg/ml) were (50, 35 mm) respectively, in Escherichia coli, inhibition zone ofsubstances concentrations (100 mg/ml, 50 mg/ml) were (40, 25 mm) respectively, also thisresults showed that three types of bacteria above were inhibited in synergistic effect, whileinhibition zone of Streptococcus pyogenes in concentration 100 mg/ml was 30 mm and inconcentration 50 mg/ml was 20 mm. |
| format | Article |
| id | doaj-art-e00a8a3de5e34a85991b589090fe481b |
| institution | Kabale University |
| issn | 1813-8497 2410-8456 |
| language | English |
| publishDate | 2018-11-01 |
| publisher | College of Veterinary Medicine, University of Basrah, Iraq |
| record_format | Article |
| series | Basrah Journal of Veterinary Research |
| spelling | doaj-art-e00a8a3de5e34a85991b589090fe481b2025-08-20T03:49:45ZengCollege of Veterinary Medicine, University of Basrah, IraqBasrah Journal of Veterinary Research1813-84972410-84562018-11-01173 (Proceeding of 6th International Scientific Conference, College of Veterinary Medicine University of Basrah, Iraq)64065110.23975/bjvetr.2018.174007174007INVESTIGATE THE ANTIBACTERIAL ACTIVITY OF AMYGDALIN AGAINST SOME TYPES OF ANTIBIOTIC RESISTANT PATHOGENIC BACTERIAAhmed A. Mhawesh0Dhafar N. Al-Ugaili1Yasameen Mad olaiwy2College of Biotechnology, Al-Nahrain University,Baghdad,IraqCollege of Biotechnology, Al-Nahrain University,Baghdad,IraqCollege of Biotechnology, Al-Nahrain University,Baghdad,IraqExtraction of Amygdalin from apple seeds by alcoholic extraction procedure were doneand applied a high-performance liquid chromatographic (HPLC) procedure for quantification ofit to investigate extraction efficiency and (16) bacterial isolates ( 4 isolates of Staphylococcusaureus, 4 isolates of Escherichia coli , 4 isolates of Pseudomonas aeruginosa and 4 isolates ofStreptococcus pyogenes from skin infections samples are choose by accomplished of drugsensitivity tests as a multidrug resistant, the antimicrobial activity of methanolic extract ofAmygdalin from crushed apple seeds was tested against each group of four pathogenic bacteriaspecies of gram positive (Staphylococcus aureus and Streptococcus pyogenes ) and gramnegative (Escherichia coli and Pseudomonas aeruginosa) by well diffusion method, the resultsshown that plant extract gave antibacterial activity through the inhibition zone , but theconcentration of the raw material was more inhibited than the dilute substance concentration,inhibition of the substance was obtained on all types of bacteria were in Staphylococcus aureus,inhibition zone of substances concentrations (100 mg/ml, 50 mg/ml) were (40, 28 mm)respectively , in Pseudomonas aeruginosa inhibition zone of substances concentrations (100mg/ml, 50 mg/ml) were (50, 35 mm) respectively, in Escherichia coli, inhibition zone ofsubstances concentrations (100 mg/ml, 50 mg/ml) were (40, 25 mm) respectively, also thisresults showed that three types of bacteria above were inhibited in synergistic effect, whileinhibition zone of Streptococcus pyogenes in concentration 100 mg/ml was 30 mm and inconcentration 50 mg/ml was 20 mm.https://bjvr.uobasrah.edu.iq/article_174007_7c6b216c4af650205a5132cf1e6c5ea6.pdf(hplc)pseudomonas aeruginosainhibition zone |
| spellingShingle | Ahmed A. Mhawesh Dhafar N. Al-Ugaili Yasameen Mad olaiwy INVESTIGATE THE ANTIBACTERIAL ACTIVITY OF AMYGDALIN AGAINST SOME TYPES OF ANTIBIOTIC RESISTANT PATHOGENIC BACTERIA Basrah Journal of Veterinary Research (hplc) pseudomonas aeruginosa inhibition zone |
| title | INVESTIGATE THE ANTIBACTERIAL ACTIVITY OF AMYGDALIN AGAINST SOME TYPES OF ANTIBIOTIC RESISTANT PATHOGENIC BACTERIA |
| title_full | INVESTIGATE THE ANTIBACTERIAL ACTIVITY OF AMYGDALIN AGAINST SOME TYPES OF ANTIBIOTIC RESISTANT PATHOGENIC BACTERIA |
| title_fullStr | INVESTIGATE THE ANTIBACTERIAL ACTIVITY OF AMYGDALIN AGAINST SOME TYPES OF ANTIBIOTIC RESISTANT PATHOGENIC BACTERIA |
| title_full_unstemmed | INVESTIGATE THE ANTIBACTERIAL ACTIVITY OF AMYGDALIN AGAINST SOME TYPES OF ANTIBIOTIC RESISTANT PATHOGENIC BACTERIA |
| title_short | INVESTIGATE THE ANTIBACTERIAL ACTIVITY OF AMYGDALIN AGAINST SOME TYPES OF ANTIBIOTIC RESISTANT PATHOGENIC BACTERIA |
| title_sort | investigate the antibacterial activity of amygdalin against some types of antibiotic resistant pathogenic bacteria |
| topic | (hplc) pseudomonas aeruginosa inhibition zone |
| url | https://bjvr.uobasrah.edu.iq/article_174007_7c6b216c4af650205a5132cf1e6c5ea6.pdf |
| work_keys_str_mv | AT ahmedamhawesh investigatetheantibacterialactivityofamygdalinagainstsometypesofantibioticresistantpathogenicbacteria AT dhafarnalugaili investigatetheantibacterialactivityofamygdalinagainstsometypesofantibioticresistantpathogenicbacteria AT yasameenmadolaiwy investigatetheantibacterialactivityofamygdalinagainstsometypesofantibioticresistantpathogenicbacteria |