The Genetic Structure of Wild Orobanche cumana Wallr. (Orobanchaceae) Populations in Eastern Bulgaria Reflects Introgressions from Weedy Populations
Orobanche cumana is a holoparasitic plant naturally distributed from central Asia to south-eastern Europe, where it parasitizes wild Asteraceae species. It is also an important parasitic weed of sunflower crops. The objective of this research was to investigate genetic diversity, population structur...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/150432 |
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| author | Rocío Pineda-Martos Antonio J. Pujadas-Salvà José M. Fernández-Martínez Kiril Stoyanov Leonardo Velasco Begoña Pérez-Vich |
| author_facet | Rocío Pineda-Martos Antonio J. Pujadas-Salvà José M. Fernández-Martínez Kiril Stoyanov Leonardo Velasco Begoña Pérez-Vich |
| author_sort | Rocío Pineda-Martos |
| collection | DOAJ |
| description | Orobanche cumana is a holoparasitic plant naturally distributed from central Asia to south-eastern Europe, where it parasitizes wild Asteraceae species. It is also an important parasitic weed of sunflower crops. The objective of this research was to investigate genetic diversity, population structure, and virulence on sunflower of O. cumana populations parasitizing wild plants in eastern Bulgaria. Fresh tissue of eight O. cumana populations and mature seeds of four of them were collected in situ on wild hosts. Genetic diversity and population structure were studied with SSR markers and compared to weedy populations. Two main gene pools were identified in Bulgarian populations, with most of the populations having intermediate characteristics. Cross-inoculation experiments revealed that O. cumana populations collected on wild species possessed similar ability to parasitize sunflower to those collected on sunflower. The results were explained on the basis of an effective genetic exchange between populations parasitizing sunflower crops and those parasitizing wild species. The occurrence of bidirectional gene flow may have an impact on wild populations, as new physiological races continuously emerge in weedy populations. Also, genetic variability of wild populations may favour the ability of weedy populations to overcome sunflower resistance mechanisms. |
| format | Article |
| id | doaj-art-dfecaa0755f943df88b4461edebd5983 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-dfecaa0755f943df88b4461edebd59832025-02-03T00:59:57ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/150432150432The Genetic Structure of Wild Orobanche cumana Wallr. (Orobanchaceae) Populations in Eastern Bulgaria Reflects Introgressions from Weedy PopulationsRocío Pineda-Martos0Antonio J. Pujadas-Salvà1José M. Fernández-Martínez2Kiril Stoyanov3Leonardo Velasco4Begoña Pérez-Vich5Institute for Sustainable Agriculture (IAS-CSIC), Finca Alameda del Obispo, Avenida Menéndez-Pidal s/n, 14004 Córdoba, SpainDepartment of Agricultural and Forestry Sciences and Resources, University of Córdoba, Campus de Rabanales, Edificio Celestino Mutis, Carretera de Madrid Km 396, 14071 Córdoba, SpainInstitute for Sustainable Agriculture (IAS-CSIC), Finca Alameda del Obispo, Avenida Menéndez-Pidal s/n, 14004 Córdoba, SpainDepartment of Botany, Agricultural University of Plovdiv, 12 Mendeleev, 4000 Plovdiv, BulgariaInstitute for Sustainable Agriculture (IAS-CSIC), Finca Alameda del Obispo, Avenida Menéndez-Pidal s/n, 14004 Córdoba, SpainInstitute for Sustainable Agriculture (IAS-CSIC), Finca Alameda del Obispo, Avenida Menéndez-Pidal s/n, 14004 Córdoba, SpainOrobanche cumana is a holoparasitic plant naturally distributed from central Asia to south-eastern Europe, where it parasitizes wild Asteraceae species. It is also an important parasitic weed of sunflower crops. The objective of this research was to investigate genetic diversity, population structure, and virulence on sunflower of O. cumana populations parasitizing wild plants in eastern Bulgaria. Fresh tissue of eight O. cumana populations and mature seeds of four of them were collected in situ on wild hosts. Genetic diversity and population structure were studied with SSR markers and compared to weedy populations. Two main gene pools were identified in Bulgarian populations, with most of the populations having intermediate characteristics. Cross-inoculation experiments revealed that O. cumana populations collected on wild species possessed similar ability to parasitize sunflower to those collected on sunflower. The results were explained on the basis of an effective genetic exchange between populations parasitizing sunflower crops and those parasitizing wild species. The occurrence of bidirectional gene flow may have an impact on wild populations, as new physiological races continuously emerge in weedy populations. Also, genetic variability of wild populations may favour the ability of weedy populations to overcome sunflower resistance mechanisms.http://dx.doi.org/10.1155/2014/150432 |
| spellingShingle | Rocío Pineda-Martos Antonio J. Pujadas-Salvà José M. Fernández-Martínez Kiril Stoyanov Leonardo Velasco Begoña Pérez-Vich The Genetic Structure of Wild Orobanche cumana Wallr. (Orobanchaceae) Populations in Eastern Bulgaria Reflects Introgressions from Weedy Populations The Scientific World Journal |
| title | The Genetic Structure of Wild Orobanche cumana Wallr. (Orobanchaceae) Populations in Eastern Bulgaria Reflects Introgressions from Weedy Populations |
| title_full | The Genetic Structure of Wild Orobanche cumana Wallr. (Orobanchaceae) Populations in Eastern Bulgaria Reflects Introgressions from Weedy Populations |
| title_fullStr | The Genetic Structure of Wild Orobanche cumana Wallr. (Orobanchaceae) Populations in Eastern Bulgaria Reflects Introgressions from Weedy Populations |
| title_full_unstemmed | The Genetic Structure of Wild Orobanche cumana Wallr. (Orobanchaceae) Populations in Eastern Bulgaria Reflects Introgressions from Weedy Populations |
| title_short | The Genetic Structure of Wild Orobanche cumana Wallr. (Orobanchaceae) Populations in Eastern Bulgaria Reflects Introgressions from Weedy Populations |
| title_sort | genetic structure of wild orobanche cumana wallr orobanchaceae populations in eastern bulgaria reflects introgressions from weedy populations |
| url | http://dx.doi.org/10.1155/2014/150432 |
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