Extracellular MMP-9-Based Assessment of Ocular Surface Inflammation in Patients with Primary Open-Angle Glaucoma
Purpose. Objective assessment of dry eye disease (DED) severity and ocular inflammation using the InflammaDry® test for extracellular matrix metalloproteinase-9 (MMP-9) and the impact of antiglaucoma eye drops in people with primary open-angle glaucoma (POAG). Methods. Overall, 90 adults (180 eyes)...
Saved in:
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2019-01-01
|
| Series: | Journal of Ophthalmology |
| Online Access: | http://dx.doi.org/10.1155/2019/1240537 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832558692642324480 |
|---|---|
| author | Anna Zaleska-Żmijewska Ewa Strzemecka Zbigniew M. Wawrzyniak Jacek P. Szaflik |
| author_facet | Anna Zaleska-Żmijewska Ewa Strzemecka Zbigniew M. Wawrzyniak Jacek P. Szaflik |
| author_sort | Anna Zaleska-Żmijewska |
| collection | DOAJ |
| description | Purpose. Objective assessment of dry eye disease (DED) severity and ocular inflammation using the InflammaDry® test for extracellular matrix metalloproteinase-9 (MMP-9) and the impact of antiglaucoma eye drops in people with primary open-angle glaucoma (POAG). Methods. Overall, 90 adults (180 eyes) were included: 60 had been diagnosed with POAG and were treated with prostaglandin analogue monotherapy and 30 were suspected of having POAG but did not receive any treatment (control group). Of those treated with prostaglandin eye drops, 30 received a preservative-free formulation (tafluprost) and 30 were treated with a formulation containing the preservative benzalkonium chloride (BAK) (latanoprost). Measurement of extracellular MMP-9 levels (InflammaDry test) provided a marker for ocular surface inflammation. Further assessments of disease severity and inflammation comprised Goldmann applanation tonometry for intraocular pressure (IOP), Schirmer’s test with anesthesia, ocular surface staining with unpreserved fluorescein (Oxford scale index), tear breakup time (TBUT), McMonnies questionnaire, and the Ocular Surface Disease index (OSDI). Results. Clinically significant MMP-9 levels (>40 ng/mL) were detected in tear film from 46.7% of subjects treated with BAK-containing medication. In contrast, only 16.7% of subjects treated with preservative-free medication or untreated individuals demonstrated similar MMP-9 levels. This difference was statistically significant (p<0.05). MMP-9 results correlated with other indicators of inflammation and disease severity. BAK-containing medication was associated with rapid TBUT (<5 seconds) in 50% of cases, while only 10% of untreated subjects and individuals using preservative-free medication demonstrated comparable TBUT results. Conclusion. Measurement of ocular surface MMP-9 level provides a useful marker for inflammation and DED in POAG. Use of a preservative-free topical prostaglandin formulation results in lower levels of ocular inflammation, compared with BAK-containing medication. |
| format | Article |
| id | doaj-art-dfcc039bf65040b2959cd683abbbb50a |
| institution | Kabale University |
| issn | 2090-004X 2090-0058 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Ophthalmology |
| spelling | doaj-art-dfcc039bf65040b2959cd683abbbb50a2025-02-03T01:31:48ZengWileyJournal of Ophthalmology2090-004X2090-00582019-01-01201910.1155/2019/12405371240537Extracellular MMP-9-Based Assessment of Ocular Surface Inflammation in Patients with Primary Open-Angle GlaucomaAnna Zaleska-Żmijewska0Ewa Strzemecka1Zbigniew M. Wawrzyniak2Jacek P. Szaflik3Department of Ophthalmology, II Faculty of Medicine, Medical University of Warsaw, Żwirki i Wigury 61, 02-091 Warsaw, PolandDepartment of Ophthalmology, II Faculty of Medicine, Medical University of Warsaw, Żwirki i Wigury 61, 02-091 Warsaw, PolandFaculty of Electronics and Information Technologies, Warsaw University of Technology, plac Politechniki 1, 00-661 Warsaw, PolandDepartment of Ophthalmology, II Faculty of Medicine, Medical University of Warsaw, Żwirki i Wigury 61, 02-091 Warsaw, PolandPurpose. Objective assessment of dry eye disease (DED) severity and ocular inflammation using the InflammaDry® test for extracellular matrix metalloproteinase-9 (MMP-9) and the impact of antiglaucoma eye drops in people with primary open-angle glaucoma (POAG). Methods. Overall, 90 adults (180 eyes) were included: 60 had been diagnosed with POAG and were treated with prostaglandin analogue monotherapy and 30 were suspected of having POAG but did not receive any treatment (control group). Of those treated with prostaglandin eye drops, 30 received a preservative-free formulation (tafluprost) and 30 were treated with a formulation containing the preservative benzalkonium chloride (BAK) (latanoprost). Measurement of extracellular MMP-9 levels (InflammaDry test) provided a marker for ocular surface inflammation. Further assessments of disease severity and inflammation comprised Goldmann applanation tonometry for intraocular pressure (IOP), Schirmer’s test with anesthesia, ocular surface staining with unpreserved fluorescein (Oxford scale index), tear breakup time (TBUT), McMonnies questionnaire, and the Ocular Surface Disease index (OSDI). Results. Clinically significant MMP-9 levels (>40 ng/mL) were detected in tear film from 46.7% of subjects treated with BAK-containing medication. In contrast, only 16.7% of subjects treated with preservative-free medication or untreated individuals demonstrated similar MMP-9 levels. This difference was statistically significant (p<0.05). MMP-9 results correlated with other indicators of inflammation and disease severity. BAK-containing medication was associated with rapid TBUT (<5 seconds) in 50% of cases, while only 10% of untreated subjects and individuals using preservative-free medication demonstrated comparable TBUT results. Conclusion. Measurement of ocular surface MMP-9 level provides a useful marker for inflammation and DED in POAG. Use of a preservative-free topical prostaglandin formulation results in lower levels of ocular inflammation, compared with BAK-containing medication.http://dx.doi.org/10.1155/2019/1240537 |
| spellingShingle | Anna Zaleska-Żmijewska Ewa Strzemecka Zbigniew M. Wawrzyniak Jacek P. Szaflik Extracellular MMP-9-Based Assessment of Ocular Surface Inflammation in Patients with Primary Open-Angle Glaucoma Journal of Ophthalmology |
| title | Extracellular MMP-9-Based Assessment of Ocular Surface Inflammation in Patients with Primary Open-Angle Glaucoma |
| title_full | Extracellular MMP-9-Based Assessment of Ocular Surface Inflammation in Patients with Primary Open-Angle Glaucoma |
| title_fullStr | Extracellular MMP-9-Based Assessment of Ocular Surface Inflammation in Patients with Primary Open-Angle Glaucoma |
| title_full_unstemmed | Extracellular MMP-9-Based Assessment of Ocular Surface Inflammation in Patients with Primary Open-Angle Glaucoma |
| title_short | Extracellular MMP-9-Based Assessment of Ocular Surface Inflammation in Patients with Primary Open-Angle Glaucoma |
| title_sort | extracellular mmp 9 based assessment of ocular surface inflammation in patients with primary open angle glaucoma |
| url | http://dx.doi.org/10.1155/2019/1240537 |
| work_keys_str_mv | AT annazaleskazmijewska extracellularmmp9basedassessmentofocularsurfaceinflammationinpatientswithprimaryopenangleglaucoma AT ewastrzemecka extracellularmmp9basedassessmentofocularsurfaceinflammationinpatientswithprimaryopenangleglaucoma AT zbigniewmwawrzyniak extracellularmmp9basedassessmentofocularsurfaceinflammationinpatientswithprimaryopenangleglaucoma AT jacekpszaflik extracellularmmp9basedassessmentofocularsurfaceinflammationinpatientswithprimaryopenangleglaucoma |