Selection of Lactobacillus strains from native chicken feces for the fermentation of purple onion (Allium cepa L.) as an antibiotic alternative against Salmonella spp. in chickens
Background: The increasing prevalence of antibiotic resistance in poultry pathogens necessitates the development of sustainable alternatives to antibiotics. Probiotics, particularly Lactobacillus spp., have shown promise in combating bacterial infections in poultry. Purple onion extract (OE) posses...
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| Format: | Article |
| Language: | English |
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Tripoli University
2024-12-01
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| Series: | Open Veterinary Journal |
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| Online Access: | http://www.ejmanager.com/fulltextpdf.php?mno=225342 |
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| author | Phan Vu Hai Hoang Thi Anh Phuong Pham Hoang Son Hung Tran Thi Na Ngo Huu Lai Nguyen Dinh Thuy Khuong Tran Ngoc Liem Nguyen Xuan Hoa |
| author_facet | Phan Vu Hai Hoang Thi Anh Phuong Pham Hoang Son Hung Tran Thi Na Ngo Huu Lai Nguyen Dinh Thuy Khuong Tran Ngoc Liem Nguyen Xuan Hoa |
| author_sort | Phan Vu Hai |
| collection | DOAJ |
| description | Background:
The increasing prevalence of antibiotic resistance in poultry pathogens necessitates the development of sustainable alternatives to antibiotics. Probiotics, particularly Lactobacillus spp., have shown promise in combating bacterial infections in poultry. Purple onion extract (OE) possesses antibacterial properties and can potentially enhance the probiotic efficacy of Lactobacillus strains.
Aim:
This study aimed to develop a biological product based on Lactobacillus-fermented OE (LFOE) as a sustainable alternative to antibiotics for the control of Salmonella-induced diarrhea in poultry.
Methods:
Lactobacillus strains were isolated from native free-range chicken feces and screened for their antibacterial activity against S. pullorum NCTC10705 and S. typhimurium FC13827, as well as their survival rate in OE. Six promising strains were selected and further characterized for their ability to ferment OE and their co-aggregation ability against the pathogenic bacteria using scanning electron microscopy. 16S rRNA gene sequencing was performed for bacterial identification. The selected strain was used for fermentation in OE, and the resulting product was freeze-dried into a biological preparation. In vivo studies in chicks were conducted to assess the safety and intestinal persistence of LFOE.
Results:
From an initial pool of 68 Lactobacillus strains, six promising candidates (L. plantarum 1582, L. plantarum WCFS1, L. plantarum JDM1, L. acidophilus NCFM, L. agilis DSM 20509, and L. agilis La3) were selected based on their antibacterial activity and high survival rate in OE. Scanning electron microscopy confirmed the ability of these strains to ferment OE and co-aggregate with pathogenic bacteria. 16S rRNA gene sequencing confirmed their taxonomic identity as Lactobacillus. L. plantarum 1582, selected for its superior probiotic properties, was used to ferment OE (LFOE), which proved safe for chicks and demonstrated the strain's ability to survive temporarily in the intestine.
Conclusion:
This study successfully developed a biopreparation based on LFOE as a potential alternative to antibiotics for the control of Salmonella-induced diarrhea in poultry. However, regular re-supplementation is required to maintain probiotic efficacy due to the transient nature of intestinal colonization. [Open Vet J 2024; 14(12.000): 3525-3538] |
| format | Article |
| id | doaj-art-df9efcbce25f48b6a3885a9d390716d2 |
| institution | OA Journals |
| issn | 2226-4485 2218-6050 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Tripoli University |
| record_format | Article |
| series | Open Veterinary Journal |
| spelling | doaj-art-df9efcbce25f48b6a3885a9d390716d22025-08-20T02:16:02ZengTripoli UniversityOpen Veterinary Journal2226-44852218-60502024-12-0114123525353810.5455/OVJ.2024.v14.i12.35225342Selection of Lactobacillus strains from native chicken feces for the fermentation of purple onion (Allium cepa L.) as an antibiotic alternative against Salmonella spp. in chickensPhan Vu Hai0Hoang Thi Anh Phuong1Pham Hoang Son Hung2Tran Thi Na3Ngo Huu Lai4Nguyen Dinh Thuy Khuong5Tran Ngoc Liem6Nguyen Xuan Hoa7Hue University of Agriculture and Forestry - 102 Phung Hung st ., Hue city, Vietnam Tay Nguyen University - 567 Le Duan Buon Ma Thuot City - Dak Lak - Vietnam Hue University of Agriculture and Forestry - 102 Phung Hung st ., Hue city, Vietnam Hue University of Agriculture and Forestry - 102 Phung Hung st ., Hue city, Vietnam Region IV Animal Health Department - 12 Tran Quy Cap, Thach Thang, Hai Chau, Da Nang Hue University of Agriculture and Forestry - 102 Phung Hung st ., Hue city, Vietnam Hue University of Agriculture and Forestry - 102 Phung Hung st ., Hue city, Vietnam Hue University of Agriculture and Forestry - 102 Phung Hung st ., Hue city, VietnamBackground: The increasing prevalence of antibiotic resistance in poultry pathogens necessitates the development of sustainable alternatives to antibiotics. Probiotics, particularly Lactobacillus spp., have shown promise in combating bacterial infections in poultry. Purple onion extract (OE) possesses antibacterial properties and can potentially enhance the probiotic efficacy of Lactobacillus strains. Aim: This study aimed to develop a biological product based on Lactobacillus-fermented OE (LFOE) as a sustainable alternative to antibiotics for the control of Salmonella-induced diarrhea in poultry. Methods: Lactobacillus strains were isolated from native free-range chicken feces and screened for their antibacterial activity against S. pullorum NCTC10705 and S. typhimurium FC13827, as well as their survival rate in OE. Six promising strains were selected and further characterized for their ability to ferment OE and their co-aggregation ability against the pathogenic bacteria using scanning electron microscopy. 16S rRNA gene sequencing was performed for bacterial identification. The selected strain was used for fermentation in OE, and the resulting product was freeze-dried into a biological preparation. In vivo studies in chicks were conducted to assess the safety and intestinal persistence of LFOE. Results: From an initial pool of 68 Lactobacillus strains, six promising candidates (L. plantarum 1582, L. plantarum WCFS1, L. plantarum JDM1, L. acidophilus NCFM, L. agilis DSM 20509, and L. agilis La3) were selected based on their antibacterial activity and high survival rate in OE. Scanning electron microscopy confirmed the ability of these strains to ferment OE and co-aggregate with pathogenic bacteria. 16S rRNA gene sequencing confirmed their taxonomic identity as Lactobacillus. L. plantarum 1582, selected for its superior probiotic properties, was used to ferment OE (LFOE), which proved safe for chicks and demonstrated the strain's ability to survive temporarily in the intestine. Conclusion: This study successfully developed a biopreparation based on LFOE as a potential alternative to antibiotics for the control of Salmonella-induced diarrhea in poultry. However, regular re-supplementation is required to maintain probiotic efficacy due to the transient nature of intestinal colonization. [Open Vet J 2024; 14(12.000): 3525-3538]http://www.ejmanager.com/fulltextpdf.php?mno=225342antibiotic resistancechickenlactobacillusonionsalmonella |
| spellingShingle | Phan Vu Hai Hoang Thi Anh Phuong Pham Hoang Son Hung Tran Thi Na Ngo Huu Lai Nguyen Dinh Thuy Khuong Tran Ngoc Liem Nguyen Xuan Hoa Selection of Lactobacillus strains from native chicken feces for the fermentation of purple onion (Allium cepa L.) as an antibiotic alternative against Salmonella spp. in chickens Open Veterinary Journal antibiotic resistance chicken lactobacillus onion salmonella |
| title | Selection of Lactobacillus strains from native chicken feces for the fermentation of purple onion (Allium cepa L.) as an antibiotic alternative against Salmonella spp. in chickens |
| title_full | Selection of Lactobacillus strains from native chicken feces for the fermentation of purple onion (Allium cepa L.) as an antibiotic alternative against Salmonella spp. in chickens |
| title_fullStr | Selection of Lactobacillus strains from native chicken feces for the fermentation of purple onion (Allium cepa L.) as an antibiotic alternative against Salmonella spp. in chickens |
| title_full_unstemmed | Selection of Lactobacillus strains from native chicken feces for the fermentation of purple onion (Allium cepa L.) as an antibiotic alternative against Salmonella spp. in chickens |
| title_short | Selection of Lactobacillus strains from native chicken feces for the fermentation of purple onion (Allium cepa L.) as an antibiotic alternative against Salmonella spp. in chickens |
| title_sort | selection of lactobacillus strains from native chicken feces for the fermentation of purple onion allium cepa l as an antibiotic alternative against salmonella spp in chickens |
| topic | antibiotic resistance chicken lactobacillus onion salmonella |
| url | http://www.ejmanager.com/fulltextpdf.php?mno=225342 |
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