BIOTECHNOLOGICAL VIEW OF ETHANOL BIOSYNTHESIS FROM MISCANTHUS
It is demonstrated that Saccharomyces сerevisiae Y-1693 yeast (RNCIM) is recalcitrant to aqueous enzymatic Miscanthus hydrolysate. Its good condition indicates that the media have no detrimental impurities characteristic of acid chemical hydrolyzates. Ethanol biosynthesis from enzymatic Miscanthus c...
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Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2015-01-01
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Series: | Вавиловский журнал генетики и селекции |
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Online Access: | https://vavilov.elpub.ru/jour/article/view/283 |
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author | O. V. Baibakova E. A. Skiba |
author_facet | O. V. Baibakova E. A. Skiba |
author_sort | O. V. Baibakova |
collection | DOAJ |
description | It is demonstrated that Saccharomyces сerevisiae Y-1693 yeast (RNCIM) is recalcitrant to aqueous enzymatic Miscanthus hydrolysate. Its good condition indicates that the media have no detrimental impurities characteristic of acid chemical hydrolyzates. Ethanol biosynthesis from enzymatic Miscanthus cellulose hydrolysate occurs best at native active pH 4,5–4,7 pH. Addition of 1 % yeast extract makes the medium of aqueous enzymatic hydrolyzate of Miscanthus cellulose complete. Moreover, in batch fermentation it is sufficient to introduce 10 % yeast inoculum in the log phase of growth. As shown by gas-liquid chromatography, enzymatic digestion of Miscanthus cellulose yields ethanol with low contents of esters and fusel oils. Miscanthus bioethanol is free of methanol. |
format | Article |
id | doaj-art-df9b559b9d4e40109e24b51ef0c2f8a8 |
institution | Kabale University |
issn | 2500-3259 |
language | English |
publishDate | 2015-01-01 |
publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
record_format | Article |
series | Вавиловский журнал генетики и селекции |
spelling | doaj-art-df9b559b9d4e40109e24b51ef0c2f8a82025-02-01T09:58:01ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592015-01-01183564571267BIOTECHNOLOGICAL VIEW OF ETHANOL BIOSYNTHESIS FROM MISCANTHUSO. V. Baibakova0E. A. Skiba1Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences, Biysk, RussiaInstitute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences, Biysk, RussiaIt is demonstrated that Saccharomyces сerevisiae Y-1693 yeast (RNCIM) is recalcitrant to aqueous enzymatic Miscanthus hydrolysate. Its good condition indicates that the media have no detrimental impurities characteristic of acid chemical hydrolyzates. Ethanol biosynthesis from enzymatic Miscanthus cellulose hydrolysate occurs best at native active pH 4,5–4,7 pH. Addition of 1 % yeast extract makes the medium of aqueous enzymatic hydrolyzate of Miscanthus cellulose complete. Moreover, in batch fermentation it is sufficient to introduce 10 % yeast inoculum in the log phase of growth. As shown by gas-liquid chromatography, enzymatic digestion of Miscanthus cellulose yields ethanol with low contents of esters and fusel oils. Miscanthus bioethanol is free of methanol.https://vavilov.elpub.ru/jour/article/view/283miscanthussoranovskii varietyenzymatic digestioncellulosesaccharomyces cerevisiae y-1693strainbioethanol |
spellingShingle | O. V. Baibakova E. A. Skiba BIOTECHNOLOGICAL VIEW OF ETHANOL BIOSYNTHESIS FROM MISCANTHUS Вавиловский журнал генетики и селекции miscanthus soranovskii variety enzymatic digestion cellulose saccharomyces cerevisiae y-1693 strain bioethanol |
title | BIOTECHNOLOGICAL VIEW OF ETHANOL BIOSYNTHESIS FROM MISCANTHUS |
title_full | BIOTECHNOLOGICAL VIEW OF ETHANOL BIOSYNTHESIS FROM MISCANTHUS |
title_fullStr | BIOTECHNOLOGICAL VIEW OF ETHANOL BIOSYNTHESIS FROM MISCANTHUS |
title_full_unstemmed | BIOTECHNOLOGICAL VIEW OF ETHANOL BIOSYNTHESIS FROM MISCANTHUS |
title_short | BIOTECHNOLOGICAL VIEW OF ETHANOL BIOSYNTHESIS FROM MISCANTHUS |
title_sort | biotechnological view of ethanol biosynthesis from miscanthus |
topic | miscanthus soranovskii variety enzymatic digestion cellulose saccharomyces cerevisiae y-1693 strain bioethanol |
url | https://vavilov.elpub.ru/jour/article/view/283 |
work_keys_str_mv | AT ovbaibakova biotechnologicalviewofethanolbiosynthesisfrommiscanthus AT easkiba biotechnologicalviewofethanolbiosynthesisfrommiscanthus |