BIOTECHNOLOGICAL VIEW OF ETHANOL BIOSYNTHESIS FROM MISCANTHUS

It is demonstrated that Saccharomyces сerevisiae Y-1693 yeast (RNCIM) is recalcitrant to aqueous enzymatic Miscanthus hydrolysate. Its good condition indicates that the media have no detrimental impurities characteristic of acid chemical hydrolyzates. Ethanol biosynthesis from enzymatic Miscanthus c...

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Main Authors: O. V. Baibakova, E. A. Skiba
Format: Article
Language:English
Published: Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders 2015-01-01
Series:Вавиловский журнал генетики и селекции
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Online Access:https://vavilov.elpub.ru/jour/article/view/283
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author O. V. Baibakova
E. A. Skiba
author_facet O. V. Baibakova
E. A. Skiba
author_sort O. V. Baibakova
collection DOAJ
description It is demonstrated that Saccharomyces сerevisiae Y-1693 yeast (RNCIM) is recalcitrant to aqueous enzymatic Miscanthus hydrolysate. Its good condition indicates that the media have no detrimental impurities characteristic of acid chemical hydrolyzates. Ethanol biosynthesis from enzymatic Miscanthus cellulose hydrolysate occurs best at native active pH 4,5–4,7 pH. Addition of 1 % yeast extract makes the medium of aqueous enzymatic hydrolyzate of Miscanthus cellulose complete. Moreover, in batch fermentation it is sufficient to introduce 10 % yeast inoculum in the log phase of growth. As shown by gas-liquid chromatography, enzymatic digestion of Miscanthus cellulose yields ethanol with low contents of esters and fusel oils. Miscanthus bioethanol is free of methanol.
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institution Kabale University
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publishDate 2015-01-01
publisher Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
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series Вавиловский журнал генетики и селекции
spelling doaj-art-df9b559b9d4e40109e24b51ef0c2f8a82025-02-01T09:58:01ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592015-01-01183564571267BIOTECHNOLOGICAL VIEW OF ETHANOL BIOSYNTHESIS FROM MISCANTHUSO. V. Baibakova0E. A. Skiba1Institute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences, Biysk, RussiaInstitute for Problems of Chemical and Energetic Technologies, Siberian Branch of the Russian Academy of Sciences, Biysk, RussiaIt is demonstrated that Saccharomyces сerevisiae Y-1693 yeast (RNCIM) is recalcitrant to aqueous enzymatic Miscanthus hydrolysate. Its good condition indicates that the media have no detrimental impurities characteristic of acid chemical hydrolyzates. Ethanol biosynthesis from enzymatic Miscanthus cellulose hydrolysate occurs best at native active pH 4,5–4,7 pH. Addition of 1 % yeast extract makes the medium of aqueous enzymatic hydrolyzate of Miscanthus cellulose complete. Moreover, in batch fermentation it is sufficient to introduce 10 % yeast inoculum in the log phase of growth. As shown by gas-liquid chromatography, enzymatic digestion of Miscanthus cellulose yields ethanol with low contents of esters and fusel oils. Miscanthus bioethanol is free of methanol.https://vavilov.elpub.ru/jour/article/view/283miscanthussoranovskii varietyenzymatic digestioncellulosesaccharomyces cerevisiae y-1693strainbioethanol
spellingShingle O. V. Baibakova
E. A. Skiba
BIOTECHNOLOGICAL VIEW OF ETHANOL BIOSYNTHESIS FROM MISCANTHUS
Вавиловский журнал генетики и селекции
miscanthus
soranovskii variety
enzymatic digestion
cellulose
saccharomyces cerevisiae y-1693
strain
bioethanol
title BIOTECHNOLOGICAL VIEW OF ETHANOL BIOSYNTHESIS FROM MISCANTHUS
title_full BIOTECHNOLOGICAL VIEW OF ETHANOL BIOSYNTHESIS FROM MISCANTHUS
title_fullStr BIOTECHNOLOGICAL VIEW OF ETHANOL BIOSYNTHESIS FROM MISCANTHUS
title_full_unstemmed BIOTECHNOLOGICAL VIEW OF ETHANOL BIOSYNTHESIS FROM MISCANTHUS
title_short BIOTECHNOLOGICAL VIEW OF ETHANOL BIOSYNTHESIS FROM MISCANTHUS
title_sort biotechnological view of ethanol biosynthesis from miscanthus
topic miscanthus
soranovskii variety
enzymatic digestion
cellulose
saccharomyces cerevisiae y-1693
strain
bioethanol
url https://vavilov.elpub.ru/jour/article/view/283
work_keys_str_mv AT ovbaibakova biotechnologicalviewofethanolbiosynthesisfrommiscanthus
AT easkiba biotechnologicalviewofethanolbiosynthesisfrommiscanthus