Integrating melt electrospinning writing and microfluidics to engineer a human cardiac microenvironment for high-fidelity drug screening
The preclinical evaluation of drug-induced cardiotoxicity is critical for developing novel drug, helping to avoid drug wastage and post-marketing withdrawal. Although human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) and the engineered heart organoid have been used for drug scree...
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KeAi Communications Co., Ltd.
2025-03-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X24005292 |
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| author | Yu-hong Wang Ting-ting Liu Yan-ping Guo Shuo-ji Zhu Zi-ming Liao Jia-mei Song Xi-ming Zhu Jia-liang Liang Moussa Ide Nasser Nan-bo Liu De-hua Chang Ping Zhu Bin Yao |
| author_facet | Yu-hong Wang Ting-ting Liu Yan-ping Guo Shuo-ji Zhu Zi-ming Liao Jia-mei Song Xi-ming Zhu Jia-liang Liang Moussa Ide Nasser Nan-bo Liu De-hua Chang Ping Zhu Bin Yao |
| author_sort | Yu-hong Wang |
| collection | DOAJ |
| description | The preclinical evaluation of drug-induced cardiotoxicity is critical for developing novel drug, helping to avoid drug wastage and post-marketing withdrawal. Although human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) and the engineered heart organoid have been used for drug screening and mimicking disease models, they are always limited by the immaturity and lack of functionality of the cardiomyocytes. In this study, we constructed a Cardiomyocytes-on-a-Chip (CoC) that combines micro-grooves (MGs) and circulating mechanical stimulation to recapitulate the well-organized structure and stable beating of myocardial tissue. The phenotypic changes and maturation of CMs cultured on the CoC have been verified and can be used for the evaluation of cardiotoxicity and cardioprotective drug responses. Taken together, these results highlight the ability of our myocardial microarray platform to accurately reflect clinical behaviour, underscoring its potential as a powerful pre-clinical tool for assessing drug response and toxicity. |
| format | Article |
| id | doaj-art-df6a1f58f4ea42bab756be4d660bbc55 |
| institution | Kabale University |
| issn | 2452-199X |
| language | English |
| publishDate | 2025-03-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj-art-df6a1f58f4ea42bab756be4d660bbc552025-01-26T05:04:26ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2025-03-0145551566Integrating melt electrospinning writing and microfluidics to engineer a human cardiac microenvironment for high-fidelity drug screeningYu-hong Wang0Ting-ting Liu1Yan-ping Guo2Shuo-ji Zhu3Zi-ming Liao4Jia-mei Song5Xi-ming Zhu6Jia-liang Liang7Moussa Ide Nasser8Nan-bo Liu9De-hua Chang10Ping Zhu11Bin Yao12Academy of Medical Engineering and Translational Medicine, Tianjin University, 300072, China; Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong, 510100, China; Guangdong Provincial Key Laboratory of Pathogenesis, Targeted Prevention and Treatment of Heart Disease, Guangzhou Key Laboratory of Cardiac Pathogenesis and Prevention, Guangdong, 510100, ChinaDepartment of Laboratory Diagnosis, The 971th Hospital, Qingdao, ChinaGuangdong Provincial Key Laboratory of Pathogenesis, Targeted Prevention and Treatment of Heart Disease, Guangzhou Key Laboratory of Cardiac Pathogenesis and Prevention, Guangdong, 510100, China; School of Medicine, South China University of Technology, 510641, ChinaGuangdong Cardiovascular Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong, 510100, China; Guangdong Provincial Key Laboratory of Pathogenesis, Targeted Prevention and Treatment of Heart Disease, Guangzhou Key Laboratory of Cardiac Pathogenesis and Prevention, Guangdong, 510100, China; Department of Cardiac Surgery, The University of Tokyo Hospital, Tokyo, 113-8654, JapanAcademy of Medical Engineering and Translational Medicine, Tianjin University, 300072, ChinaAcademy of Medical Engineering and Translational Medicine, Tianjin University, 300072, ChinaAcademy of Medical Engineering and Translational Medicine, Tianjin University, 300072, ChinaGuangdong Cardiovascular Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong, 510100, China; Guangdong Provincial Key Laboratory of Pathogenesis, Targeted Prevention and Treatment of Heart Disease, Guangzhou Key Laboratory of Cardiac Pathogenesis and Prevention, Guangdong, 510100, ChinaGuangdong Cardiovascular Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong, 510100, China; Guangdong Provincial Key Laboratory of Pathogenesis, Targeted Prevention and Treatment of Heart Disease, Guangzhou Key Laboratory of Cardiac Pathogenesis and Prevention, Guangdong, 510100, ChinaGuangdong Cardiovascular Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong, 510100, China; Guangdong Provincial Key Laboratory of Pathogenesis, Targeted Prevention and Treatment of Heart Disease, Guangzhou Key Laboratory of Cardiac Pathogenesis and Prevention, Guangdong, 510100, China; Corresponding author. Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong, 510100, China.Department of Cell Therapy in Regenerative Medicine, University of Tokyo Hospital, Tokyo, 113-8654, Japan; Corresponding author.Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong, 510100, China; Guangdong Provincial Key Laboratory of Pathogenesis, Targeted Prevention and Treatment of Heart Disease, Guangzhou Key Laboratory of Cardiac Pathogenesis and Prevention, Guangdong, 510100, China; Corresponding author. Guangdong Cardiovascular Institute, Guangdong Provincial People's Hospital (Guangdong Academy of Medical Sciences), Southern Medical University, Guangzhou, Guangdong, 510100, China.Academy of Medical Engineering and Translational Medicine, Tianjin University, 300072, China; Corresponding author.The preclinical evaluation of drug-induced cardiotoxicity is critical for developing novel drug, helping to avoid drug wastage and post-marketing withdrawal. Although human induced pluripotent stem cell-derived cardiomyocytes (iPSC-CMs) and the engineered heart organoid have been used for drug screening and mimicking disease models, they are always limited by the immaturity and lack of functionality of the cardiomyocytes. In this study, we constructed a Cardiomyocytes-on-a-Chip (CoC) that combines micro-grooves (MGs) and circulating mechanical stimulation to recapitulate the well-organized structure and stable beating of myocardial tissue. The phenotypic changes and maturation of CMs cultured on the CoC have been verified and can be used for the evaluation of cardiotoxicity and cardioprotective drug responses. Taken together, these results highlight the ability of our myocardial microarray platform to accurately reflect clinical behaviour, underscoring its potential as a powerful pre-clinical tool for assessing drug response and toxicity.http://www.sciencedirect.com/science/article/pii/S2452199X24005292iPSC-CMsCardiac tissue engineeringMicro-groovesMechanical stretchingMicrofluidicsDrug screening |
| spellingShingle | Yu-hong Wang Ting-ting Liu Yan-ping Guo Shuo-ji Zhu Zi-ming Liao Jia-mei Song Xi-ming Zhu Jia-liang Liang Moussa Ide Nasser Nan-bo Liu De-hua Chang Ping Zhu Bin Yao Integrating melt electrospinning writing and microfluidics to engineer a human cardiac microenvironment for high-fidelity drug screening Bioactive Materials iPSC-CMs Cardiac tissue engineering Micro-grooves Mechanical stretching Microfluidics Drug screening |
| title | Integrating melt electrospinning writing and microfluidics to engineer a human cardiac microenvironment for high-fidelity drug screening |
| title_full | Integrating melt electrospinning writing and microfluidics to engineer a human cardiac microenvironment for high-fidelity drug screening |
| title_fullStr | Integrating melt electrospinning writing and microfluidics to engineer a human cardiac microenvironment for high-fidelity drug screening |
| title_full_unstemmed | Integrating melt electrospinning writing and microfluidics to engineer a human cardiac microenvironment for high-fidelity drug screening |
| title_short | Integrating melt electrospinning writing and microfluidics to engineer a human cardiac microenvironment for high-fidelity drug screening |
| title_sort | integrating melt electrospinning writing and microfluidics to engineer a human cardiac microenvironment for high fidelity drug screening |
| topic | iPSC-CMs Cardiac tissue engineering Micro-grooves Mechanical stretching Microfluidics Drug screening |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X24005292 |
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