Immunization with a Synthetic Helicobacter pylori Peptide Induces Secretory IgA Antibodies and Protects Mice against Infection
Helicobacter pylori is a spiral Gram-negative bacterium associated with inflammation of the gastric mucosa, peptic ulcer, and gastric adenocarcinoma, whose treatment has failed due to antibiotic resistance and side effects. Furthermore, because there are no vaccines effective against H. pylori, an a...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Canadian Journal of Infectious Diseases and Medical Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2019/8595487 |
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| author | David Espinosa-Ramos Diana Caballero-Hernández Ricardo Gomez-Flores Armando Trejo-Chávez Luis Jerónimo Pérez-Limón Myriam Angélica de la Garza-Ramos Reyes Tamez-Guerra Patricia Tamez-Guerra Cristina Rodriguez-Padilla |
| author_facet | David Espinosa-Ramos Diana Caballero-Hernández Ricardo Gomez-Flores Armando Trejo-Chávez Luis Jerónimo Pérez-Limón Myriam Angélica de la Garza-Ramos Reyes Tamez-Guerra Patricia Tamez-Guerra Cristina Rodriguez-Padilla |
| author_sort | David Espinosa-Ramos |
| collection | DOAJ |
| description | Helicobacter pylori is a spiral Gram-negative bacterium associated with inflammation of the gastric mucosa, peptic ulcer, and gastric adenocarcinoma, whose treatment has failed due to antibiotic resistance and side effects. Furthermore, because there are no vaccines effective against H. pylori, an appropriate vaccine design targeting conserved/essential genes must be identified. In the present study, a H. pylori 50–52 kDa immunogen-derived peptide antigen with the sequence Met-Val-Thr-Leu-Ile-Asn-Asn-Glu (MVTLINNE) was used to immunize against H. pylori infection. For this, mice received an intraperitoneal injection of 100 μg of H. pylori peptide on the first week, followed by two weekly subcutaneous reinforcements and further 109 bacteria administration in the drinking water for 3 weeks. Thymic cells proliferative responses to concanavalin A, serum levels of IL-2, IL-4, IL-6, IL-10, IL-17, IFN-γ, and TNF-α cytokines, and IgG1, IgG2a, IgG2b, IgG3 IgM, and IgA immunoglobulins were evaluated. Significant (p<0.05) increases on lymphoproliferation and spleen weights after immunization were observed. In contrast, infection significantly (p<0.05) decreased lymphoproliferation, which was recovered in immunized mice. In addition, levels of serum TH1 and TH2 cytokines were not altered after immunization, except for the significant increase in IL-6 production in immunized and/or infected animals. Moreover, immunization correlated with plasma secretory IgA and IgG, whereas infection alone only elicited IgM antibodies. Peptide immunization protected 100% of mice against virulent H. pylori. MVTLINNE peptide deserves further research as an approach to the prophylaxis of H. pylori infection. |
| format | Article |
| id | doaj-art-df4b16f0fb284c67b86f77b4a8a54ba7 |
| institution | Kabale University |
| issn | 1712-9532 1918-1493 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Canadian Journal of Infectious Diseases and Medical Microbiology |
| spelling | doaj-art-df4b16f0fb284c67b86f77b4a8a54ba72025-02-03T01:31:18ZengWileyCanadian Journal of Infectious Diseases and Medical Microbiology1712-95321918-14932019-01-01201910.1155/2019/85954878595487Immunization with a Synthetic Helicobacter pylori Peptide Induces Secretory IgA Antibodies and Protects Mice against InfectionDavid Espinosa-Ramos0Diana Caballero-Hernández1Ricardo Gomez-Flores2Armando Trejo-Chávez3Luis Jerónimo Pérez-Limón4Myriam Angélica de la Garza-Ramos5Reyes Tamez-Guerra6Patricia Tamez-Guerra7Cristina Rodriguez-Padilla8Universidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Departamento de Microbiología e Inmunología, San Nicolás de los Garza, NL. C.P. 66450, MexicoUniversidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Departamento de Microbiología e Inmunología, San Nicolás de los Garza, NL. C.P. 66450, MexicoUniversidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Departamento de Microbiología e Inmunología, San Nicolás de los Garza, NL. C.P. 66450, MexicoUniversidad Autónoma de Nuevo León, Facultad de Medicina Veterinaria y Zootecnia, Departamento de Patobiología, Campus de Ciencias Agropecuarias, Escobedo, NL. C.P. 66050, MexicoUniversidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Departamento de Microbiología e Inmunología, San Nicolás de los Garza, NL. C.P. 66450, MexicoUniversidad Autónoma de Nuevo León, Facultad de Odontología y Centro de Investigación y Desarrollo en Ciencias de la Salud, Unidad de Odontología Integral y Especialidades, Av. Dr. Aguirre Pequeño y Silao S/N, Monterrey, NL. C.P. 64460, MexicoUniversidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Departamento de Microbiología e Inmunología, San Nicolás de los Garza, NL. C.P. 66450, MexicoUniversidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Departamento de Microbiología e Inmunología, San Nicolás de los Garza, NL. C.P. 66450, MexicoUniversidad Autónoma de Nuevo León, Facultad de Ciencias Biológicas, Departamento de Microbiología e Inmunología, San Nicolás de los Garza, NL. C.P. 66450, MexicoHelicobacter pylori is a spiral Gram-negative bacterium associated with inflammation of the gastric mucosa, peptic ulcer, and gastric adenocarcinoma, whose treatment has failed due to antibiotic resistance and side effects. Furthermore, because there are no vaccines effective against H. pylori, an appropriate vaccine design targeting conserved/essential genes must be identified. In the present study, a H. pylori 50–52 kDa immunogen-derived peptide antigen with the sequence Met-Val-Thr-Leu-Ile-Asn-Asn-Glu (MVTLINNE) was used to immunize against H. pylori infection. For this, mice received an intraperitoneal injection of 100 μg of H. pylori peptide on the first week, followed by two weekly subcutaneous reinforcements and further 109 bacteria administration in the drinking water for 3 weeks. Thymic cells proliferative responses to concanavalin A, serum levels of IL-2, IL-4, IL-6, IL-10, IL-17, IFN-γ, and TNF-α cytokines, and IgG1, IgG2a, IgG2b, IgG3 IgM, and IgA immunoglobulins were evaluated. Significant (p<0.05) increases on lymphoproliferation and spleen weights after immunization were observed. In contrast, infection significantly (p<0.05) decreased lymphoproliferation, which was recovered in immunized mice. In addition, levels of serum TH1 and TH2 cytokines were not altered after immunization, except for the significant increase in IL-6 production in immunized and/or infected animals. Moreover, immunization correlated with plasma secretory IgA and IgG, whereas infection alone only elicited IgM antibodies. Peptide immunization protected 100% of mice against virulent H. pylori. MVTLINNE peptide deserves further research as an approach to the prophylaxis of H. pylori infection.http://dx.doi.org/10.1155/2019/8595487 |
| spellingShingle | David Espinosa-Ramos Diana Caballero-Hernández Ricardo Gomez-Flores Armando Trejo-Chávez Luis Jerónimo Pérez-Limón Myriam Angélica de la Garza-Ramos Reyes Tamez-Guerra Patricia Tamez-Guerra Cristina Rodriguez-Padilla Immunization with a Synthetic Helicobacter pylori Peptide Induces Secretory IgA Antibodies and Protects Mice against Infection Canadian Journal of Infectious Diseases and Medical Microbiology |
| title | Immunization with a Synthetic Helicobacter pylori Peptide Induces Secretory IgA Antibodies and Protects Mice against Infection |
| title_full | Immunization with a Synthetic Helicobacter pylori Peptide Induces Secretory IgA Antibodies and Protects Mice against Infection |
| title_fullStr | Immunization with a Synthetic Helicobacter pylori Peptide Induces Secretory IgA Antibodies and Protects Mice against Infection |
| title_full_unstemmed | Immunization with a Synthetic Helicobacter pylori Peptide Induces Secretory IgA Antibodies and Protects Mice against Infection |
| title_short | Immunization with a Synthetic Helicobacter pylori Peptide Induces Secretory IgA Antibodies and Protects Mice against Infection |
| title_sort | immunization with a synthetic helicobacter pylori peptide induces secretory iga antibodies and protects mice against infection |
| url | http://dx.doi.org/10.1155/2019/8595487 |
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