Quantitative glycoproteomics of human milk and association with atopic disease.
The prevalence of allergic diseases and asthma is increasing rapidly worldwide, with environmental and lifestyle behaviors implicated as a reason. Epidemiological studies have shown that children who grow up on farms are at lower risk of developing childhood atopic disease, indicating the presence o...
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Public Library of Science (PLoS)
2022-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0267967&type=printable |
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| author | Matilda Holm Mayank Saraswat Sakari Joenväärä Antti Seppo R John Looney Tiialotta Tohmola Jutta Renkonen Risto Renkonen Kirsi M Järvinen |
| author_facet | Matilda Holm Mayank Saraswat Sakari Joenväärä Antti Seppo R John Looney Tiialotta Tohmola Jutta Renkonen Risto Renkonen Kirsi M Järvinen |
| author_sort | Matilda Holm |
| collection | DOAJ |
| description | The prevalence of allergic diseases and asthma is increasing rapidly worldwide, with environmental and lifestyle behaviors implicated as a reason. Epidemiological studies have shown that children who grow up on farms are at lower risk of developing childhood atopic disease, indicating the presence of a protective "farm effect". The Old Order Mennonite (OOM) community in Upstate New York have traditional, agrarian lifestyles, a low rate of atopic disease, and long periods of exclusive breastfeeding. Human milk proteins are heavily glycosylated, although there is a paucity of studies investigating the milk glycoproteome. In this study, we have used quantitative glycoproteomics to compare the N-glycoprotein profiles of 54 milk samples from Rochester urban/suburban and OOM mothers, two populations with different lifestyles, exposures, and risk of atopic disease. We also compared N-glycoprotein profiles according to the presence or absence of atopic disease in the mothers and, separately, the children. We identified 79 N-glycopeptides from 15 different proteins and found that proteins including immunoglobulin A1, polymeric immunoglobulin receptor, and lactotransferrin displayed significant glycan heterogeneity. We found that the abundances of 38 glycopeptides differed significantly between Rochester and OOM mothers and also identified four glycopeptides with significantly different abundances between all comparisons. These four glycopeptides may be associated with the development of atopic disease. The findings of this study suggest that the differential glycosylation of milk proteins could be linked to atopic disease. |
| format | Article |
| id | doaj-art-df45d76c55524df8b056584dfaa81a9e |
| institution | Kabale University |
| issn | 1932-6203 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-df45d76c55524df8b056584dfaa81a9e2025-01-21T05:31:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01175e026796710.1371/journal.pone.0267967Quantitative glycoproteomics of human milk and association with atopic disease.Matilda HolmMayank SaraswatSakari JoenvääräAntti SeppoR John LooneyTiialotta TohmolaJutta RenkonenRisto RenkonenKirsi M JärvinenThe prevalence of allergic diseases and asthma is increasing rapidly worldwide, with environmental and lifestyle behaviors implicated as a reason. Epidemiological studies have shown that children who grow up on farms are at lower risk of developing childhood atopic disease, indicating the presence of a protective "farm effect". The Old Order Mennonite (OOM) community in Upstate New York have traditional, agrarian lifestyles, a low rate of atopic disease, and long periods of exclusive breastfeeding. Human milk proteins are heavily glycosylated, although there is a paucity of studies investigating the milk glycoproteome. In this study, we have used quantitative glycoproteomics to compare the N-glycoprotein profiles of 54 milk samples from Rochester urban/suburban and OOM mothers, two populations with different lifestyles, exposures, and risk of atopic disease. We also compared N-glycoprotein profiles according to the presence or absence of atopic disease in the mothers and, separately, the children. We identified 79 N-glycopeptides from 15 different proteins and found that proteins including immunoglobulin A1, polymeric immunoglobulin receptor, and lactotransferrin displayed significant glycan heterogeneity. We found that the abundances of 38 glycopeptides differed significantly between Rochester and OOM mothers and also identified four glycopeptides with significantly different abundances between all comparisons. These four glycopeptides may be associated with the development of atopic disease. The findings of this study suggest that the differential glycosylation of milk proteins could be linked to atopic disease.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0267967&type=printable |
| spellingShingle | Matilda Holm Mayank Saraswat Sakari Joenväärä Antti Seppo R John Looney Tiialotta Tohmola Jutta Renkonen Risto Renkonen Kirsi M Järvinen Quantitative glycoproteomics of human milk and association with atopic disease. PLoS ONE |
| title | Quantitative glycoproteomics of human milk and association with atopic disease. |
| title_full | Quantitative glycoproteomics of human milk and association with atopic disease. |
| title_fullStr | Quantitative glycoproteomics of human milk and association with atopic disease. |
| title_full_unstemmed | Quantitative glycoproteomics of human milk and association with atopic disease. |
| title_short | Quantitative glycoproteomics of human milk and association with atopic disease. |
| title_sort | quantitative glycoproteomics of human milk and association with atopic disease |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0267967&type=printable |
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