Characterization of a new transposon, Tn7772, on a novel plasmid from multidrug-resistant Escherichia coli in China
ABSTRACT: Background: The emergence of multidrug-resistant (MDR) Escherichia coli causes a serious threat to human and animal health. To characterize the genetic environments of a novel plasmid pQL57EC-1 in an MDR E. coli strain QL57EC from feces of a healthy swine in China. Methods: The plasmid pQ...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-09-01
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| Series: | Journal of Global Antimicrobial Resistance |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2213716525001675 |
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| author | Yong Pan Ting Li Yanan Zhang Qiufan Xu Li Yang Yuanfeng Zhao Jinge Xu |
| author_facet | Yong Pan Ting Li Yanan Zhang Qiufan Xu Li Yang Yuanfeng Zhao Jinge Xu |
| author_sort | Yong Pan |
| collection | DOAJ |
| description | ABSTRACT: Background: The emergence of multidrug-resistant (MDR) Escherichia coli causes a serious threat to human and animal health. To characterize the genetic environments of a novel plasmid pQL57EC-1 in an MDR E. coli strain QL57EC from feces of a healthy swine in China. Methods: The plasmid pQL57EC-1 was characterized by antimicrobial susceptibility testing, whole genome sequencing and bioinformatic analysis. The transferability of pQL57EC-1 was verified by conjugation experiments and transformation experiments. Results: E. coli QL57EC carries two plasmids. pQL57EC-1 belongs to the incompatibility groups IncF-FIB/IncX1, while plasmid pQL57EC-2 belongs to the incompatibility group IncF-FIB. Notably, pQL57EC-1 was a novel plasmid, containing a novel Tn7772 transposon flanked by two copies of IS26 at both ends, which can also be found on plasmids from Salmonella enterica isolated from human feces. Conjugation assays for E. coli QL57EC were unsuccessful. However, pQL57EC-1 could be transferred into E. coli DH5α by transformation experiments. Conclusions: This study reported a novel plasmid pQL57EC-1 harboring a novel Tn7772 transposon from swine derived E. coli QL57EC.This finding suggests that Tn7772 may pose a threat to both human and animal health by acting as a reservoir for antibiotic resistance genes (ARGs) and facilitating cross-species transmission. |
| format | Article |
| id | doaj-art-df3ba529b2f94e1cb064bf7528aa1698 |
| institution | Kabale University |
| issn | 2213-7165 |
| language | English |
| publishDate | 2025-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Global Antimicrobial Resistance |
| spelling | doaj-art-df3ba529b2f94e1cb064bf7528aa16982025-08-20T03:46:50ZengElsevierJournal of Global Antimicrobial Resistance2213-71652025-09-014433233910.1016/j.jgar.2025.07.010Characterization of a new transposon, Tn7772, on a novel plasmid from multidrug-resistant Escherichia coli in ChinaYong Pan0Ting Li1Yanan Zhang2Qiufan Xu3Li Yang4Yuanfeng Zhao5Jinge Xu6Institute of Animal Husbandry and Veterinary Medicine, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou, ChinaGuangdong Institute of Modern Agricultural Equipment, Guangzhou, Guangdong, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou, ChinaInstitute of Animal Husbandry and Veterinary Medicine, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou, China; Corresponding author. Institute of Animal Husbandry and Veterinary Medicine, Guizhou Academy of Agricultural Sciences, Guiyang, Guizhou, China.ABSTRACT: Background: The emergence of multidrug-resistant (MDR) Escherichia coli causes a serious threat to human and animal health. To characterize the genetic environments of a novel plasmid pQL57EC-1 in an MDR E. coli strain QL57EC from feces of a healthy swine in China. Methods: The plasmid pQL57EC-1 was characterized by antimicrobial susceptibility testing, whole genome sequencing and bioinformatic analysis. The transferability of pQL57EC-1 was verified by conjugation experiments and transformation experiments. Results: E. coli QL57EC carries two plasmids. pQL57EC-1 belongs to the incompatibility groups IncF-FIB/IncX1, while plasmid pQL57EC-2 belongs to the incompatibility group IncF-FIB. Notably, pQL57EC-1 was a novel plasmid, containing a novel Tn7772 transposon flanked by two copies of IS26 at both ends, which can also be found on plasmids from Salmonella enterica isolated from human feces. Conjugation assays for E. coli QL57EC were unsuccessful. However, pQL57EC-1 could be transferred into E. coli DH5α by transformation experiments. Conclusions: This study reported a novel plasmid pQL57EC-1 harboring a novel Tn7772 transposon from swine derived E. coli QL57EC.This finding suggests that Tn7772 may pose a threat to both human and animal health by acting as a reservoir for antibiotic resistance genes (ARGs) and facilitating cross-species transmission.http://www.sciencedirect.com/science/article/pii/S2213716525001675E. coliPlasmidAntibiotic resistance genesWhole genome sequencingTransposon |
| spellingShingle | Yong Pan Ting Li Yanan Zhang Qiufan Xu Li Yang Yuanfeng Zhao Jinge Xu Characterization of a new transposon, Tn7772, on a novel plasmid from multidrug-resistant Escherichia coli in China Journal of Global Antimicrobial Resistance E. coli Plasmid Antibiotic resistance genes Whole genome sequencing Transposon |
| title | Characterization of a new transposon, Tn7772, on a novel plasmid from multidrug-resistant Escherichia coli in China |
| title_full | Characterization of a new transposon, Tn7772, on a novel plasmid from multidrug-resistant Escherichia coli in China |
| title_fullStr | Characterization of a new transposon, Tn7772, on a novel plasmid from multidrug-resistant Escherichia coli in China |
| title_full_unstemmed | Characterization of a new transposon, Tn7772, on a novel plasmid from multidrug-resistant Escherichia coli in China |
| title_short | Characterization of a new transposon, Tn7772, on a novel plasmid from multidrug-resistant Escherichia coli in China |
| title_sort | characterization of a new transposon tn7772 on a novel plasmid from multidrug resistant escherichia coli in china |
| topic | E. coli Plasmid Antibiotic resistance genes Whole genome sequencing Transposon |
| url | http://www.sciencedirect.com/science/article/pii/S2213716525001675 |
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