24-Epibrassinolide, a Phytosterol from the Brassinosteroid Family, Protects Dopaminergic Cells against MPP+-Induced Oxidative Stress and Apoptosis
Oxidative stress and apoptosis are frequently cited to explain neuronal cell damage in various neurodegenerative disorders, such as Parkinson' s disease. Brassinosteroids (BRs) are phytosterols recognized to promote stress tolerance of vegetables via modulation of the antioxidative enzyme casca...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Journal of Toxicology |
| Online Access: | http://dx.doi.org/10.1155/2011/392859 |
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| author | Julie Carange Fanny Longpré Benoit Daoust Maria-Grazia Martinoli |
| author_facet | Julie Carange Fanny Longpré Benoit Daoust Maria-Grazia Martinoli |
| author_sort | Julie Carange |
| collection | DOAJ |
| description | Oxidative stress and apoptosis are frequently cited to explain neuronal cell damage in various neurodegenerative disorders, such as Parkinson' s disease. Brassinosteroids (BRs) are phytosterols recognized to promote stress tolerance of vegetables via modulation of the antioxidative enzyme cascade. However, their antioxidative effects on mammalian neuronal cells have never been examined so far.
We analyzed the ability of 24-epibrassinolide (24-Epi), a natural BR, to protect neuronal PC12 cells from 1-methyl-4-phenylpyridinium- (MPP+-) induced oxidative stress and consequent apoptosis in dopaminergic neurons. Our results demonstrate that 24-Epi reduces the levels of intracellular reactive oxygen species and modulates superoxide dismutase, catalase, and glutathione peroxidase activities. Finally, we determined that the antioxidative properties of 24-Epi lead to the inhibition of MPP+-induced apoptosis by reducing DNA fragmentation as well as the Bax/Bcl-2 protein ratio and cleaved caspase-3. This is the first time that the potent antioxidant and neuroprotective role of 24-Epi has been shown in a mammalian neuronal cell line. |
| format | Article |
| id | doaj-art-df399e362be94254aa3235f9dd603e78 |
| institution | Kabale University |
| issn | 1687-8191 1687-8205 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Toxicology |
| spelling | doaj-art-df399e362be94254aa3235f9dd603e782025-02-03T01:24:14ZengWileyJournal of Toxicology1687-81911687-82052011-01-01201110.1155/2011/39285939285924-Epibrassinolide, a Phytosterol from the Brassinosteroid Family, Protects Dopaminergic Cells against MPP+-Induced Oxidative Stress and ApoptosisJulie Carange0Fanny Longpré1Benoit Daoust2Maria-Grazia Martinoli3Department of Biochemistry, Neurosciences Research Group, Université du Québec à Trois-Rivières, Trois-Rivières, QC, G9A 5H7, CanadaDepartment of Biochemistry, Neurosciences Research Group, Université du Québec à Trois-Rivières, Trois-Rivières, QC, G9A 5H7, CanadaDepartment of Biochemistry, Neurosciences Research Group, Université du Québec à Trois-Rivières, Trois-Rivières, QC, G9A 5H7, CanadaDepartment of Biochemistry, Neurosciences Research Group, Université du Québec à Trois-Rivières, Trois-Rivières, QC, G9A 5H7, CanadaOxidative stress and apoptosis are frequently cited to explain neuronal cell damage in various neurodegenerative disorders, such as Parkinson' s disease. Brassinosteroids (BRs) are phytosterols recognized to promote stress tolerance of vegetables via modulation of the antioxidative enzyme cascade. However, their antioxidative effects on mammalian neuronal cells have never been examined so far. We analyzed the ability of 24-epibrassinolide (24-Epi), a natural BR, to protect neuronal PC12 cells from 1-methyl-4-phenylpyridinium- (MPP+-) induced oxidative stress and consequent apoptosis in dopaminergic neurons. Our results demonstrate that 24-Epi reduces the levels of intracellular reactive oxygen species and modulates superoxide dismutase, catalase, and glutathione peroxidase activities. Finally, we determined that the antioxidative properties of 24-Epi lead to the inhibition of MPP+-induced apoptosis by reducing DNA fragmentation as well as the Bax/Bcl-2 protein ratio and cleaved caspase-3. This is the first time that the potent antioxidant and neuroprotective role of 24-Epi has been shown in a mammalian neuronal cell line.http://dx.doi.org/10.1155/2011/392859 |
| spellingShingle | Julie Carange Fanny Longpré Benoit Daoust Maria-Grazia Martinoli 24-Epibrassinolide, a Phytosterol from the Brassinosteroid Family, Protects Dopaminergic Cells against MPP+-Induced Oxidative Stress and Apoptosis Journal of Toxicology |
| title | 24-Epibrassinolide, a Phytosterol from the Brassinosteroid Family, Protects Dopaminergic Cells against MPP+-Induced Oxidative Stress and Apoptosis |
| title_full | 24-Epibrassinolide, a Phytosterol from the Brassinosteroid Family, Protects Dopaminergic Cells against MPP+-Induced Oxidative Stress and Apoptosis |
| title_fullStr | 24-Epibrassinolide, a Phytosterol from the Brassinosteroid Family, Protects Dopaminergic Cells against MPP+-Induced Oxidative Stress and Apoptosis |
| title_full_unstemmed | 24-Epibrassinolide, a Phytosterol from the Brassinosteroid Family, Protects Dopaminergic Cells against MPP+-Induced Oxidative Stress and Apoptosis |
| title_short | 24-Epibrassinolide, a Phytosterol from the Brassinosteroid Family, Protects Dopaminergic Cells against MPP+-Induced Oxidative Stress and Apoptosis |
| title_sort | 24 epibrassinolide a phytosterol from the brassinosteroid family protects dopaminergic cells against mpp induced oxidative stress and apoptosis |
| url | http://dx.doi.org/10.1155/2011/392859 |
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