Development and validation of a new mpox virus sequencing and bioinformatic analysis pipeline

Development and validation of a new mpox virus sequencing and bioinformatic analysis pipeline

Sequencing and bioinformatic analysis of mpox virus (MPXV) remain challenging in resource-limited settings. We developed and validated a PCR-based sequencing assay that targets a 12.5 kilobase (kb) region that is phylogenetically representative of the whole ∼ 200 kb MPXV genome. We combined this seq...

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Bibliographic Details
Main Authors: Missiani Ochwoto, Skyler Kuhn, Paul Schaughency, Ben Greene, Kailin Hawes, Felix Koukouikila-Koussounda, Reiche Golmard Elenga, Dachel Aymard Eyenet Boussam, Pembe Issamou Mayangue, Jonathan Schulz, Craig Martens, Fabien Roch Niama, Justin Lack, Ryan F. Relich, Vincent J. Munster, Claude Kwe Yinda
Format: Article
Language:English
Published: Taylor & Francis Group 2025-12-01
Series:Emerging Microbes and Infections
Subjects:
Mpox
MPXV
clades
PCR
sequencing
bioinformatics pipeline
Online Access:https://www.tandfonline.com/doi/10.1080/22221751.2025.2494733
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