Effects of dietary supplementation with fish-derived Bacillus amyloliquefaciens AV5 on growth status, immune response, microbiota, and intestinal health of Nile tilapia (Oreochromis niloticus)
In this study, the effects of the probiotic Bacillus amyloliquefaciens AV5 on Nile tilapia (Oreochromis niloticus) at three different concentrations (GC: 0 cfu/g feed, G1: 1 ×106 cfu/g feed, and G2:1 ×108 cfu/g feed) on growth conditions, antioxidant responses, microbiota, and intestinal structure w...
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Elsevier
2025-04-01
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| Series: | Aquaculture Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352513425000444 |
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| author | Vicent Michael Shija Huapu Chen Yi Li Lishuko Ng’onga Kwaku Amoah Zhong Yong Junxi Chen Yu Dapeng Robert Mkuye Jia Cai |
| author_facet | Vicent Michael Shija Huapu Chen Yi Li Lishuko Ng’onga Kwaku Amoah Zhong Yong Junxi Chen Yu Dapeng Robert Mkuye Jia Cai |
| author_sort | Vicent Michael Shija |
| collection | DOAJ |
| description | In this study, the effects of the probiotic Bacillus amyloliquefaciens AV5 on Nile tilapia (Oreochromis niloticus) at three different concentrations (GC: 0 cfu/g feed, G1: 1 ×106 cfu/g feed, and G2:1 ×108 cfu/g feed) on growth conditions, antioxidant responses, microbiota, and intestinal structure were assessed. Nile Tilapia fingerlings (23.5 g ± 0.2 g) were provided with commercial feed containing different doses of B. amyloliquefaciens AV5 over 42 days. The results showed substantial improvements in initial weight, weight gain rate, survival rate, condition factor, intestinal somatic index, hepatosomatic index, and specific growth rate in the G2 group compared to the GC and G1 groups. However, the feed conversion ratios were notably higher in G1 and GC than in G2. Alkaline phosphatase and acid phosphatase activities increased considerably in G2 compared with GC and G1, but alanine aminotransferase and aspartate aminotransferase levels increased significantly in GC and G1 compared with G2. The most abundant bacteria at the phylum level are Proteobacteria, followed by Fusobacteriota, Actinobacteriota, Bacteroidetes, and Firmicutes. At the class level, there was a notably higher presence of Bacteroidia and Bacilli in groups G1 and G2 than in GC, indicating the potential suppression of harmful bacteria within the G1 and G2 diet groups. Improved intestinal morphology was observed in the G2 group. In G2, the fish livers changed from abnormally shaped to normally shaped nuclei and the gaps within the liver tissues decreased. These findings indicate that G2 can significantly enhance Nile tilapia growth. |
| format | Article |
| id | doaj-art-dedb5fb700d449318a4fc9adaa7a262f |
| institution | Kabale University |
| issn | 2352-5134 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aquaculture Reports |
| spelling | doaj-art-dedb5fb700d449318a4fc9adaa7a262f2025-02-02T05:27:43ZengElsevierAquaculture Reports2352-51342025-04-0141102658Effects of dietary supplementation with fish-derived Bacillus amyloliquefaciens AV5 on growth status, immune response, microbiota, and intestinal health of Nile tilapia (Oreochromis niloticus)Vicent Michael Shija0Huapu Chen1Yi Li2Lishuko Ng’onga3Kwaku Amoah4Zhong Yong5Junxi Chen6Yu Dapeng7Robert Mkuye8Jia Cai9Shenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China; College of Fishery, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524088, ChinaCollege of Fishery, Guangdong Ocean University, Zhanjiang 524088, ChinaCollege of Fishery, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524088, ChinaCollege of Fishery, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524088, ChinaCollege of Fishery, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524088, ChinaCollege of Fishery, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524088, ChinaCollege of Fishery, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524088, ChinaCollege of Fishery, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524088, ChinaCollege of Fishery, Guangdong Ocean University, Zhanjiang 524088, ChinaShenzhen Institute of Guangdong Ocean University, Shenzhen 518120, China; College of Fishery, Guangdong Ocean University, Zhanjiang 524088, China; Guangdong Provincial Key Laboratory of Aquatic Animal Disease Control and Healthy Culture, Zhanjiang 524088, China; Guangdong Key Laboratory of Control for Diseases of Aquatic Economic Animals, Zhanjiang 524088, China; Southern Marine Science and Engineering Guangdong Laboratory, Zhanjiang 524002, China; Guangxi Key Laboratory for Marine Natural Products and Combinational Biosynthesis Chemistry, Nanning 530200, China; Guangxi Beibu Gulf Marine Research Centre, Guangxi Academy of Sciences, Nanning 530007, China; Correspondence to: Fisheries College, Guangdong Ocean University, No. 1 of Haida Road, Mazhang District, Zhanjiang, Guangdong Province 524088, China.In this study, the effects of the probiotic Bacillus amyloliquefaciens AV5 on Nile tilapia (Oreochromis niloticus) at three different concentrations (GC: 0 cfu/g feed, G1: 1 ×106 cfu/g feed, and G2:1 ×108 cfu/g feed) on growth conditions, antioxidant responses, microbiota, and intestinal structure were assessed. Nile Tilapia fingerlings (23.5 g ± 0.2 g) were provided with commercial feed containing different doses of B. amyloliquefaciens AV5 over 42 days. The results showed substantial improvements in initial weight, weight gain rate, survival rate, condition factor, intestinal somatic index, hepatosomatic index, and specific growth rate in the G2 group compared to the GC and G1 groups. However, the feed conversion ratios were notably higher in G1 and GC than in G2. Alkaline phosphatase and acid phosphatase activities increased considerably in G2 compared with GC and G1, but alanine aminotransferase and aspartate aminotransferase levels increased significantly in GC and G1 compared with G2. The most abundant bacteria at the phylum level are Proteobacteria, followed by Fusobacteriota, Actinobacteriota, Bacteroidetes, and Firmicutes. At the class level, there was a notably higher presence of Bacteroidia and Bacilli in groups G1 and G2 than in GC, indicating the potential suppression of harmful bacteria within the G1 and G2 diet groups. Improved intestinal morphology was observed in the G2 group. In G2, the fish livers changed from abnormally shaped to normally shaped nuclei and the gaps within the liver tissues decreased. These findings indicate that G2 can significantly enhance Nile tilapia growth.http://www.sciencedirect.com/science/article/pii/S2352513425000444Bacillus amyloliquefaciens AV5GrowthGut microbiotaHistologyOreochromis niloticus |
| spellingShingle | Vicent Michael Shija Huapu Chen Yi Li Lishuko Ng’onga Kwaku Amoah Zhong Yong Junxi Chen Yu Dapeng Robert Mkuye Jia Cai Effects of dietary supplementation with fish-derived Bacillus amyloliquefaciens AV5 on growth status, immune response, microbiota, and intestinal health of Nile tilapia (Oreochromis niloticus) Aquaculture Reports Bacillus amyloliquefaciens AV5 Growth Gut microbiota Histology Oreochromis niloticus |
| title | Effects of dietary supplementation with fish-derived Bacillus amyloliquefaciens AV5 on growth status, immune response, microbiota, and intestinal health of Nile tilapia (Oreochromis niloticus) |
| title_full | Effects of dietary supplementation with fish-derived Bacillus amyloliquefaciens AV5 on growth status, immune response, microbiota, and intestinal health of Nile tilapia (Oreochromis niloticus) |
| title_fullStr | Effects of dietary supplementation with fish-derived Bacillus amyloliquefaciens AV5 on growth status, immune response, microbiota, and intestinal health of Nile tilapia (Oreochromis niloticus) |
| title_full_unstemmed | Effects of dietary supplementation with fish-derived Bacillus amyloliquefaciens AV5 on growth status, immune response, microbiota, and intestinal health of Nile tilapia (Oreochromis niloticus) |
| title_short | Effects of dietary supplementation with fish-derived Bacillus amyloliquefaciens AV5 on growth status, immune response, microbiota, and intestinal health of Nile tilapia (Oreochromis niloticus) |
| title_sort | effects of dietary supplementation with fish derived bacillus amyloliquefaciens av5 on growth status immune response microbiota and intestinal health of nile tilapia oreochromis niloticus |
| topic | Bacillus amyloliquefaciens AV5 Growth Gut microbiota Histology Oreochromis niloticus |
| url | http://www.sciencedirect.com/science/article/pii/S2352513425000444 |
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