Qualitative and Quantitative Evaluation of Chemical Constituents from Shuanghuanglian Injection Using Nuclear Magnetic Resonance Spectroscopy
By employing nuclear magnetic resonance (NMR), we implemented a chemical research on Shuanghuanglian injection (SHLI) and identified 17 components, including eight primary metabolites and nine secondary metabolites. Guided by the approach of network pharmacology, the potential activities were briefl...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2022-01-01
|
| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2022/7763207 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832551498531209216 |
|---|---|
| author | Ziyan Wang Zuoyuan Wang Miaomiao Jiang Jing Yang Qingfen Meng Jianli Guan Maoling Xu Xin Chai |
| author_facet | Ziyan Wang Zuoyuan Wang Miaomiao Jiang Jing Yang Qingfen Meng Jianli Guan Maoling Xu Xin Chai |
| author_sort | Ziyan Wang |
| collection | DOAJ |
| description | By employing nuclear magnetic resonance (NMR), we implemented a chemical research on Shuanghuanglian injection (SHLI) and identified 17 components, including eight primary metabolites and nine secondary metabolites. Guided by the approach of network pharmacology, the potential activities were briefly predicted for seven primary metabolites except for formic acid, such as anti-inflammation, antioxidation, and cardiovascular protection. The focused primary metabolites were quantified by a proton nuclear magnetic resonance (1H-NMR) method, which was verified with good linearity and satisfactory precision, repeatability, stability, and accuracy (except for myo-inositol with mean recovery at 135.78%). Based on the successfully established method, seven primary metabolites were effectively quantified with a slight fluctuation in 20 batches of SHLIs. The average total content of these compounds was 6.85 mg/mL, accounting for 24.84% in total solid of SHLI. This research provides an alternative method for analysis of primary metabolites and contributes to the quality control of SHLI. |
| format | Article |
| id | doaj-art-dea9d7b181664070aee222a6314e8990 |
| institution | Kabale University |
| issn | 2090-8873 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-dea9d7b181664070aee222a6314e89902025-02-03T06:01:25ZengWileyJournal of Analytical Methods in Chemistry2090-88732022-01-01202210.1155/2022/7763207Qualitative and Quantitative Evaluation of Chemical Constituents from Shuanghuanglian Injection Using Nuclear Magnetic Resonance SpectroscopyZiyan Wang0Zuoyuan Wang1Miaomiao Jiang2Jing Yang3Qingfen Meng4Jianli Guan5Maoling Xu6Xin Chai7State Key Laboratory of Component-based Chinese MedicineState Key Laboratory of Component-based Chinese MedicineState Key Laboratory of Component-based Chinese MedicineState Key Laboratory of Component-based Chinese MedicineHenan Fusen Pharmaceutical Co.,Ltd.Henan Fusen Pharmaceutical Co.,Ltd.Tianjin Medical University General HospitalState Key Laboratory of Component-based Chinese MedicineBy employing nuclear magnetic resonance (NMR), we implemented a chemical research on Shuanghuanglian injection (SHLI) and identified 17 components, including eight primary metabolites and nine secondary metabolites. Guided by the approach of network pharmacology, the potential activities were briefly predicted for seven primary metabolites except for formic acid, such as anti-inflammation, antioxidation, and cardiovascular protection. The focused primary metabolites were quantified by a proton nuclear magnetic resonance (1H-NMR) method, which was verified with good linearity and satisfactory precision, repeatability, stability, and accuracy (except for myo-inositol with mean recovery at 135.78%). Based on the successfully established method, seven primary metabolites were effectively quantified with a slight fluctuation in 20 batches of SHLIs. The average total content of these compounds was 6.85 mg/mL, accounting for 24.84% in total solid of SHLI. This research provides an alternative method for analysis of primary metabolites and contributes to the quality control of SHLI.http://dx.doi.org/10.1155/2022/7763207 |
| spellingShingle | Ziyan Wang Zuoyuan Wang Miaomiao Jiang Jing Yang Qingfen Meng Jianli Guan Maoling Xu Xin Chai Qualitative and Quantitative Evaluation of Chemical Constituents from Shuanghuanglian Injection Using Nuclear Magnetic Resonance Spectroscopy Journal of Analytical Methods in Chemistry |
| title | Qualitative and Quantitative Evaluation of Chemical Constituents from Shuanghuanglian Injection Using Nuclear Magnetic Resonance Spectroscopy |
| title_full | Qualitative and Quantitative Evaluation of Chemical Constituents from Shuanghuanglian Injection Using Nuclear Magnetic Resonance Spectroscopy |
| title_fullStr | Qualitative and Quantitative Evaluation of Chemical Constituents from Shuanghuanglian Injection Using Nuclear Magnetic Resonance Spectroscopy |
| title_full_unstemmed | Qualitative and Quantitative Evaluation of Chemical Constituents from Shuanghuanglian Injection Using Nuclear Magnetic Resonance Spectroscopy |
| title_short | Qualitative and Quantitative Evaluation of Chemical Constituents from Shuanghuanglian Injection Using Nuclear Magnetic Resonance Spectroscopy |
| title_sort | qualitative and quantitative evaluation of chemical constituents from shuanghuanglian injection using nuclear magnetic resonance spectroscopy |
| url | http://dx.doi.org/10.1155/2022/7763207 |
| work_keys_str_mv | AT ziyanwang qualitativeandquantitativeevaluationofchemicalconstituentsfromshuanghuanglianinjectionusingnuclearmagneticresonancespectroscopy AT zuoyuanwang qualitativeandquantitativeevaluationofchemicalconstituentsfromshuanghuanglianinjectionusingnuclearmagneticresonancespectroscopy AT miaomiaojiang qualitativeandquantitativeevaluationofchemicalconstituentsfromshuanghuanglianinjectionusingnuclearmagneticresonancespectroscopy AT jingyang qualitativeandquantitativeevaluationofchemicalconstituentsfromshuanghuanglianinjectionusingnuclearmagneticresonancespectroscopy AT qingfenmeng qualitativeandquantitativeevaluationofchemicalconstituentsfromshuanghuanglianinjectionusingnuclearmagneticresonancespectroscopy AT jianliguan qualitativeandquantitativeevaluationofchemicalconstituentsfromshuanghuanglianinjectionusingnuclearmagneticresonancespectroscopy AT maolingxu qualitativeandquantitativeevaluationofchemicalconstituentsfromshuanghuanglianinjectionusingnuclearmagneticresonancespectroscopy AT xinchai qualitativeandquantitativeevaluationofchemicalconstituentsfromshuanghuanglianinjectionusingnuclearmagneticresonancespectroscopy |