Identification of T and В lymphocytes in chickens and their subpopulations byflowcytofluorometric analysis using flow cytometer BD FACSVerse™
Method of identification ofT- and B-lymphocytes in chickens and their subpopulations by flow cytofluorometric analysis using BD FACSVerseTM was developed. Method of lymphocytic gate extraction with control of leukocyte common antigen CD45 and monocytic antigen expression was determined. Antibody pan...
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| Format: | Article |
| Language: | English |
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Da Vinci Media
2018-04-01
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| Series: | Ветеринария сегодня |
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| Online Access: | https://veterinary.arriah.ru/jour/article/view/194 |
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| author | M. A. Volkova Ir. A. Chvala |
| author_facet | M. A. Volkova Ir. A. Chvala |
| author_sort | M. A. Volkova |
| collection | DOAJ |
| description | Method of identification ofT- and B-lymphocytes in chickens and their subpopulations by flow cytofluorometric analysis using BD FACSVerseTM was developed. Method of lymphocytic gate extraction with control of leukocyte common antigen CD45 and monocytic antigen expression was determined. Antibody panels for immunophenotyping lymphocytes when testing chicken cell mediated immune response were selected and tested. They enable to determine a relative number of T- and B-lymphocytes, T-helpers and T-cytotoxic cell populations in blood and chicken lymphoid organs. The paper demonstrates specificity and reproducibility of the developed method of identification of chicken lymphocyte subpopulation. |
| format | Article |
| id | doaj-art-de83649460cd415ab800a4f7af1c71ab |
| institution | Kabale University |
| issn | 2304-196X 2658-6959 |
| language | English |
| publishDate | 2018-04-01 |
| publisher | Da Vinci Media |
| record_format | Article |
| series | Ветеринария сегодня |
| spelling | doaj-art-de83649460cd415ab800a4f7af1c71ab2025-02-06T09:52:05ZengDa Vinci MediaВетеринария сегодня2304-196X2658-69592018-04-01024347194Identification of T and В lymphocytes in chickens and their subpopulations byflowcytofluorometric analysis using flow cytometer BD FACSVerse™M. A. Volkova0Ir. A. Chvala1FGBI "ARRIAH", VladimirFGBI "ARRIAH", VladimirMethod of identification ofT- and B-lymphocytes in chickens and their subpopulations by flow cytofluorometric analysis using BD FACSVerseTM was developed. Method of lymphocytic gate extraction with control of leukocyte common antigen CD45 and monocytic antigen expression was determined. Antibody panels for immunophenotyping lymphocytes when testing chicken cell mediated immune response were selected and tested. They enable to determine a relative number of T- and B-lymphocytes, T-helpers and T-cytotoxic cell populations in blood and chicken lymphoid organs. The paper demonstrates specificity and reproducibility of the developed method of identification of chicken lymphocyte subpopulation.https://veterinary.arriah.ru/jour/article/view/194flow cytofluorometric analysisimmunophenotyping of chicken lymphocytes |
| spellingShingle | M. A. Volkova Ir. A. Chvala Identification of T and В lymphocytes in chickens and their subpopulations byflowcytofluorometric analysis using flow cytometer BD FACSVerse™ Ветеринария сегодня flow cytofluorometric analysis immunophenotyping of chicken lymphocytes |
| title | Identification of T and В lymphocytes in chickens and their subpopulations byflowcytofluorometric analysis using flow cytometer BD FACSVerse™ |
| title_full | Identification of T and В lymphocytes in chickens and their subpopulations byflowcytofluorometric analysis using flow cytometer BD FACSVerse™ |
| title_fullStr | Identification of T and В lymphocytes in chickens and their subpopulations byflowcytofluorometric analysis using flow cytometer BD FACSVerse™ |
| title_full_unstemmed | Identification of T and В lymphocytes in chickens and their subpopulations byflowcytofluorometric analysis using flow cytometer BD FACSVerse™ |
| title_short | Identification of T and В lymphocytes in chickens and their subpopulations byflowcytofluorometric analysis using flow cytometer BD FACSVerse™ |
| title_sort | identification of t and в lymphocytes in chickens and their subpopulations byflowcytofluorometric analysis using flow cytometer bd facsverse™ |
| topic | flow cytofluorometric analysis immunophenotyping of chicken lymphocytes |
| url | https://veterinary.arriah.ru/jour/article/view/194 |
| work_keys_str_mv | AT mavolkova identificationoftandvlymphocytesinchickensandtheirsubpopulationsbyflowcytofluorometricanalysisusingflowcytometerbdfacsverse AT irachvala identificationoftandvlymphocytesinchickensandtheirsubpopulationsbyflowcytofluorometricanalysisusingflowcytometerbdfacsverse |