Advantages of more stressful cold test procedure for evaluation of maize seed vigour
Cold test (CT) is the oldest vigour test, which is widely used for testing maize seed. CT has not been validated and it is not included in the ISTA Rules, due to the variations in the soil used as a substrate and differences in test procedures. The objective of this study was to compare two CT proce...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Serbian Association of Plant Breedings and Seed Producers
2024-01-01
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| Series: | Selekcija i Semenarstvo |
| Subjects: | |
| Online Access: | https://scindeks-clanci.ceon.rs/data/pdf/0354-5881/2024/0354-58812402031M.pdf |
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| Summary: | Cold test (CT) is the oldest vigour test, which is widely used for testing maize seed. CT has not been validated and it is not included in the ISTA Rules, due to the variations in the soil used as a substrate and differences in test procedures. The objective of this study was to compare two CT procedures by evaluation the seed vigour of 12 maize seed lots. Soil was used in both cases with differences in chilling temperature (10 °C in standard and 7.5 °C in severe CT). Also duration of low temperatures was prolonged from 7 (standard CT) to 10 days (severe CT). Germination at optimal conditions were the same (7 days at 20<=>30°C). Statistical analysis showed that seed lots differed significantly in seed vigour. The application of severe CT created larger differences between lots, but they maintained similar rank positions in both CT procedures, leading to a very high rank correlation. Results of this research show that standard CT is a good indicator of maize seed vigour, but severe CT is useful for clearer differentiation of maize seed lots. |
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| ISSN: | 0354-5881 2406-209X |