Congenital Zika Syndrome: Insights from Integrated Proteomic and Metabolomic Analysis
<b>Background:</b> In this study, we investigated the role of extracellular vesicles (EVs) in the pathogenesis of Congenital Zika Syndrome (CZS). Previous studies have highlighted the role of EVs in intercellular communication and the modulation of biological processes during viral infec...
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2024-12-01
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| author | Leticia Gomes-de-Pontes Lucila Akune Barreiros Lillian Nunes Gomes Ranieri Coelho Salgado Sarah Maria da Silva Napoleão Paulo V. Soeiro-Pereira Saulo Duarte Passos Antonio Condino-Neto |
| author_facet | Leticia Gomes-de-Pontes Lucila Akune Barreiros Lillian Nunes Gomes Ranieri Coelho Salgado Sarah Maria da Silva Napoleão Paulo V. Soeiro-Pereira Saulo Duarte Passos Antonio Condino-Neto |
| author_sort | Leticia Gomes-de-Pontes |
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| description | <b>Background:</b> In this study, we investigated the role of extracellular vesicles (EVs) in the pathogenesis of Congenital Zika Syndrome (CZS). Previous studies have highlighted the role of EVs in intercellular communication and the modulation of biological processes during viral infections, motivating our in-depth analysis. Our objective was to identify specific molecular signatures in the EVs of patients with CZS, focusing on their potential as biomarkers and on cellular pathways affected by the infection. <b>Methods:</b> We conducted advanced proteomic and metabolomic analyses using mass spectrometry for protein and metabolite identification. EVs were isolated from CZS patient samples and control groups using Izon qEV size-exclusion chromatography columns. <b>Results:</b> The analyzed EVs presented distinct molecular profiles in patients with CZS. Proteomic analysis revealed significant alterations in specific proteins, suggesting involvement in the PI3K-AKT-mTOR pathway, while metabolomics highlighted metabolites related to critical processes in Zika virus pathogenesis. These findings suggest a key role for the PI3K-AKT-mTOR pathway in regulating cellular processes during infection and indicate the involvement of EVs in intercellular communication. Additionally, the results identified potential biomarkers capable of aiding early diagnosis and assessing disease progression. <b>Conclusions:</b> This study demonstrates that EVs play a crucial role in intercellular communication during Zika virus infection. The identification of specific alterations in the PI3K-AKT-mTOR pathway highlights a possible therapeutic target, providing new opportunities for the development of more effective treatment strategies for CZS. Our findings significantly advance the understanding of CZS and underscore the need for further investigations using advanced techniques to validate and explore these potential molecular targets. |
| format | Article |
| id | doaj-art-de2943509b7e4e94a51112441935de0a |
| institution | Kabale University |
| issn | 2218-273X |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Biomolecules |
| spelling | doaj-art-de2943509b7e4e94a51112441935de0a2025-01-24T13:24:55ZengMDPI AGBiomolecules2218-273X2024-12-011513210.3390/biom15010032Congenital Zika Syndrome: Insights from Integrated Proteomic and Metabolomic AnalysisLeticia Gomes-de-Pontes0Lucila Akune Barreiros1Lillian Nunes Gomes2Ranieri Coelho Salgado3Sarah Maria da Silva Napoleão4Paulo V. Soeiro-Pereira5Saulo Duarte Passos6Antonio Condino-Neto7Department of Immunology (LIH), Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 2415-Butantã, São Paulo 05508-000, SP, BrazilDepartment of Immunology (LIH), Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 2415-Butantã, São Paulo 05508-000, SP, BrazilDepartment of Immunology (LIH), Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 2415-Butantã, São Paulo 05508-000, SP, BrazilDepartment of Immunology (LIH), Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 2415-Butantã, São Paulo 05508-000, SP, BrazilDepartment of Immunology (LIH), Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 2415-Butantã, São Paulo 05508-000, SP, BrazilDepartment of Pathology, Federal University of Maranhão, São Luís 65065-545, MA, BrazilInfectious Pediatric Laboratory, Medicine School of Jundiaí, Jundiaí 13202-550, SP, BrazilDepartment of Immunology (LIH), Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 2415-Butantã, São Paulo 05508-000, SP, Brazil<b>Background:</b> In this study, we investigated the role of extracellular vesicles (EVs) in the pathogenesis of Congenital Zika Syndrome (CZS). Previous studies have highlighted the role of EVs in intercellular communication and the modulation of biological processes during viral infections, motivating our in-depth analysis. Our objective was to identify specific molecular signatures in the EVs of patients with CZS, focusing on their potential as biomarkers and on cellular pathways affected by the infection. <b>Methods:</b> We conducted advanced proteomic and metabolomic analyses using mass spectrometry for protein and metabolite identification. EVs were isolated from CZS patient samples and control groups using Izon qEV size-exclusion chromatography columns. <b>Results:</b> The analyzed EVs presented distinct molecular profiles in patients with CZS. Proteomic analysis revealed significant alterations in specific proteins, suggesting involvement in the PI3K-AKT-mTOR pathway, while metabolomics highlighted metabolites related to critical processes in Zika virus pathogenesis. These findings suggest a key role for the PI3K-AKT-mTOR pathway in regulating cellular processes during infection and indicate the involvement of EVs in intercellular communication. Additionally, the results identified potential biomarkers capable of aiding early diagnosis and assessing disease progression. <b>Conclusions:</b> This study demonstrates that EVs play a crucial role in intercellular communication during Zika virus infection. The identification of specific alterations in the PI3K-AKT-mTOR pathway highlights a possible therapeutic target, providing new opportunities for the development of more effective treatment strategies for CZS. Our findings significantly advance the understanding of CZS and underscore the need for further investigations using advanced techniques to validate and explore these potential molecular targets.https://www.mdpi.com/2218-273X/15/1/32Zika infectionextracellularvesiclesproteomemetabolome |
| spellingShingle | Leticia Gomes-de-Pontes Lucila Akune Barreiros Lillian Nunes Gomes Ranieri Coelho Salgado Sarah Maria da Silva Napoleão Paulo V. Soeiro-Pereira Saulo Duarte Passos Antonio Condino-Neto Congenital Zika Syndrome: Insights from Integrated Proteomic and Metabolomic Analysis Biomolecules Zika infection extracellular vesicles proteome metabolome |
| title | Congenital Zika Syndrome: Insights from Integrated Proteomic and Metabolomic Analysis |
| title_full | Congenital Zika Syndrome: Insights from Integrated Proteomic and Metabolomic Analysis |
| title_fullStr | Congenital Zika Syndrome: Insights from Integrated Proteomic and Metabolomic Analysis |
| title_full_unstemmed | Congenital Zika Syndrome: Insights from Integrated Proteomic and Metabolomic Analysis |
| title_short | Congenital Zika Syndrome: Insights from Integrated Proteomic and Metabolomic Analysis |
| title_sort | congenital zika syndrome insights from integrated proteomic and metabolomic analysis |
| topic | Zika infection extracellular vesicles proteome metabolome |
| url | https://www.mdpi.com/2218-273X/15/1/32 |
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