A Chronic Implant to Record Electroretinogram, Visual Evoked Potentials and Oscillatory Potentials in Awake, Freely Moving Rats for Pharmacological Studies
Electroretinogram (ERG), widely used to study the pharmacological effects of drugs in animal models (e.g., diabetic retinopathy), is usually recorded in anesthetized rats. We report here a novel simple method to obtain chronic implantation of electrodes for simultaneous recording at the retinal and...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Wiley
2004-01-01
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| Series: | Neural Plasticity |
| Online Access: | http://dx.doi.org/10.1155/NP.2004.241 |
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| Summary: | Electroretinogram (ERG), widely used to
study the pharmacological effects of drugs in
animal models (e.g., diabetic retinopathy), is
usually recorded in anesthetized rats. We report
here a novel simple method to obtain chronic
implantation of electrodes for simultaneous
recording at the retinal and cortical levels in
freely moving, unanesthetized animals. We
recorded cortical (VEPs) and retinal (ERGs)
responses evoked by light (flash) stimuli in
awake rats and compared the results in the
same rats anesthetized with urethane (0.6
mg/kg) before and after the monocular administration
of scopolamine methyl bromide (1
solution). We also compared the retinal
responses with those derived from a classic acute
corneal electrode. Anesthesia induced consistent
changes of several VEP and ERG parameters
like an increase of both latency and amplitude.
In particular, the analysis of the variation of
latency, amplitude, and spectral content of
rapid oscillatory potentials could be important
for a functional evaluation of the visual system
in unanesthetized versus anesthetized animals. |
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| ISSN: | 2090-5904 1687-5443 |