Detection of viral antigen and inflammatory mediators in fatal pediatric dengue: a study on lung immunopathogenesis
IntroductionThe dengue virus (DENV) is the etiological agent that causes dengue fever illness, an arbovirus with a major endemic potential that has become increasingly prevalent in Brazil and has already been associated with fatal cases in children. DENV has tropism for several organs, including lun...
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Frontiers Media S.A.
2025-02-01
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| author | Leandro Junqueira Moragas Laíza Vianna Arruda Laíza Vianna Arruda Lucca de Lima Siqueira Oliveira Felipe de Andrade Vieira Alves Felipe de Andrade Vieira Alves Natália Gedeão Salomão Natália Gedeão Salomão Jemima Fuentes Ribeiro da Silva Carlos Alberto Basílio-de-Oliveira Rodrigo Panno Basílio-de-Oliveira Ronaldo Mohana-Borges Caio Gonçalves Azevedo Gabriela Xavier de Oliveira Jorge José de Carvalho Fernando Colonna Rosman Fernando Colonna Rosman Marciano Viana Paes Kíssila Rabelo Kíssila Rabelo |
| author_facet | Leandro Junqueira Moragas Laíza Vianna Arruda Laíza Vianna Arruda Lucca de Lima Siqueira Oliveira Felipe de Andrade Vieira Alves Felipe de Andrade Vieira Alves Natália Gedeão Salomão Natália Gedeão Salomão Jemima Fuentes Ribeiro da Silva Carlos Alberto Basílio-de-Oliveira Rodrigo Panno Basílio-de-Oliveira Ronaldo Mohana-Borges Caio Gonçalves Azevedo Gabriela Xavier de Oliveira Jorge José de Carvalho Fernando Colonna Rosman Fernando Colonna Rosman Marciano Viana Paes Kíssila Rabelo Kíssila Rabelo |
| author_sort | Leandro Junqueira Moragas |
| collection | DOAJ |
| description | IntroductionThe dengue virus (DENV) is the etiological agent that causes dengue fever illness, an arbovirus with a major endemic potential that has become increasingly prevalent in Brazil and has already been associated with fatal cases in children. DENV has tropism for several organs, including lungs causing pulmonary complications. The aim of this article was to evaluate the inflammatory and histopathological profile of the lung tissue of three fatal cases of children infected with DENV, which represents a group more susceptible to fatality due to its incomplete development.MethodsHistopathological analysis was carried out using Hematoxylin and Eosin staining and special stains. While the characterization of the inflammatory response and cellular expression was done by marking the viral protein, macrophages, lymphocytes and pro-inflammatory cytokines.Results and discussionThe results confirm that vascular dysfunctions such as hemorrhage, vascular congestion and edema associated with a mononuclear infiltrate were observed in all three cases. In addition, the presence of viral replication and increased expression of inflammatory markers were also observed. Such findings contribute to the study and description of dengue, especially its effects on lung tissue. |
| format | Article |
| id | doaj-art-de1712cfbd514c4d9c91d8f7688cf918 |
| institution | Kabale University |
| issn | 1664-3224 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj-art-de1712cfbd514c4d9c91d8f7688cf9182025-02-04T06:32:03ZengFrontiers Media S.A.Frontiers in Immunology1664-32242025-02-011610.3389/fimmu.2025.14872841487284Detection of viral antigen and inflammatory mediators in fatal pediatric dengue: a study on lung immunopathogenesisLeandro Junqueira Moragas0Laíza Vianna Arruda1Laíza Vianna Arruda2Lucca de Lima Siqueira Oliveira3Felipe de Andrade Vieira Alves4Felipe de Andrade Vieira Alves5Natália Gedeão Salomão6Natália Gedeão Salomão7Jemima Fuentes Ribeiro da Silva8Carlos Alberto Basílio-de-Oliveira9Rodrigo Panno Basílio-de-Oliveira10Ronaldo Mohana-Borges11Caio Gonçalves Azevedo12Gabriela Xavier de Oliveira13Jorge José de Carvalho14Fernando Colonna Rosman15Fernando Colonna Rosman16Marciano Viana Paes17Kíssila Rabelo18Kíssila Rabelo19Laboratório Interdisciplinar de Pesquisas Médicas, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, BrazilLaboratório Interdisciplinar de Pesquisas Médicas, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, BrazilLaboratório de Ultraestrutura e Biologia Tecidual, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório Interdisciplinar de Pesquisas Médicas, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, BrazilLaboratório Interdisciplinar de Pesquisas Médicas, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, BrazilLaboratório de Ultraestrutura e Biologia Tecidual, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório Interdisciplinar de Pesquisas Médicas, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, BrazilLaboratório das Interações Vírus-Hospedeiros, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, BrazilLaboratório de Ultraestrutura e Biologia Tecidual, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, BrazilDepartamento de Patologia, Faculdade de Medicina, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilDepartamento de Patologia, Faculdade de Medicina, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório de Genômica Estrutural, Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório de Ultraestrutura e Biologia Tecidual, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório de Ultraestrutura e Biologia Tecidual, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, BrazilLaboratório de Ultraestrutura e Biologia Tecidual, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, BrazilDepartamento de Patologia, Faculdade de Medicina, Universidade Federal do Rio de Janeiro, Rio de Janeiro, BrazilServiço de Anatomia Patológica, Hospital Municipal Jesus, Rio de Janeiro, BrazilLaboratório Interdisciplinar de Pesquisas Médicas, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, BrazilLaboratório Interdisciplinar de Pesquisas Médicas, Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, BrazilLaboratório de Ultraestrutura e Biologia Tecidual, Universidade do Estado do Rio de Janeiro, Rio de Janeiro, BrazilIntroductionThe dengue virus (DENV) is the etiological agent that causes dengue fever illness, an arbovirus with a major endemic potential that has become increasingly prevalent in Brazil and has already been associated with fatal cases in children. DENV has tropism for several organs, including lungs causing pulmonary complications. The aim of this article was to evaluate the inflammatory and histopathological profile of the lung tissue of three fatal cases of children infected with DENV, which represents a group more susceptible to fatality due to its incomplete development.MethodsHistopathological analysis was carried out using Hematoxylin and Eosin staining and special stains. While the characterization of the inflammatory response and cellular expression was done by marking the viral protein, macrophages, lymphocytes and pro-inflammatory cytokines.Results and discussionThe results confirm that vascular dysfunctions such as hemorrhage, vascular congestion and edema associated with a mononuclear infiltrate were observed in all three cases. In addition, the presence of viral replication and increased expression of inflammatory markers were also observed. Such findings contribute to the study and description of dengue, especially its effects on lung tissue.https://www.frontiersin.org/articles/10.3389/fimmu.2025.1487284/fulldengue viruschildrenhistopathologylunginflammation |
| spellingShingle | Leandro Junqueira Moragas Laíza Vianna Arruda Laíza Vianna Arruda Lucca de Lima Siqueira Oliveira Felipe de Andrade Vieira Alves Felipe de Andrade Vieira Alves Natália Gedeão Salomão Natália Gedeão Salomão Jemima Fuentes Ribeiro da Silva Carlos Alberto Basílio-de-Oliveira Rodrigo Panno Basílio-de-Oliveira Ronaldo Mohana-Borges Caio Gonçalves Azevedo Gabriela Xavier de Oliveira Jorge José de Carvalho Fernando Colonna Rosman Fernando Colonna Rosman Marciano Viana Paes Kíssila Rabelo Kíssila Rabelo Detection of viral antigen and inflammatory mediators in fatal pediatric dengue: a study on lung immunopathogenesis Frontiers in Immunology dengue virus children histopathology lung inflammation |
| title | Detection of viral antigen and inflammatory mediators in fatal pediatric dengue: a study on lung immunopathogenesis |
| title_full | Detection of viral antigen and inflammatory mediators in fatal pediatric dengue: a study on lung immunopathogenesis |
| title_fullStr | Detection of viral antigen and inflammatory mediators in fatal pediatric dengue: a study on lung immunopathogenesis |
| title_full_unstemmed | Detection of viral antigen and inflammatory mediators in fatal pediatric dengue: a study on lung immunopathogenesis |
| title_short | Detection of viral antigen and inflammatory mediators in fatal pediatric dengue: a study on lung immunopathogenesis |
| title_sort | detection of viral antigen and inflammatory mediators in fatal pediatric dengue a study on lung immunopathogenesis |
| topic | dengue virus children histopathology lung inflammation |
| url | https://www.frontiersin.org/articles/10.3389/fimmu.2025.1487284/full |
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