Endocrine and Metabolic Signaling in Retroperitoneal White Adipose Tissue Remodeling during Cold Acclimation
The expression profiles of adiponectin, resistin, 5′-AMP-activated protein kinase α (AMPKα), hypoxia-inducible factor-1α (HIF-1α), and key enzymes of glucose and fatty acid metabolism and oxidative phosphorylation in rat retroperitoneal white adipose tissue (RpWAT) during 45-day cold acclimation wer...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Obesity |
| Online Access: | http://dx.doi.org/10.1155/2013/937572 |
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| author | Aleksandra Jankovic Aleksandra Korac Biljana Buzadzic Vesna Otasevic Ana Stancic Milica Vucetic Milica Markelic Ksenija Velickovic Igor Golic Bato Korac |
| author_facet | Aleksandra Jankovic Aleksandra Korac Biljana Buzadzic Vesna Otasevic Ana Stancic Milica Vucetic Milica Markelic Ksenija Velickovic Igor Golic Bato Korac |
| author_sort | Aleksandra Jankovic |
| collection | DOAJ |
| description | The expression profiles of adiponectin, resistin, 5′-AMP-activated protein kinase α (AMPKα), hypoxia-inducible factor-1α (HIF-1α), and key enzymes of glucose and fatty acid metabolism and oxidative phosphorylation in rat retroperitoneal white adipose tissue (RpWAT) during 45-day cold acclimation were examined. After transient suppression on day 1, adiponectin protein level increased following sustained cold exposure. In parallel, on day 1, the protein level of HIF-1α was strongly induced and AMPKα suppressed, while afterwards the reverse was seen. What is more, after an initial decrease on day 1, a sequential increase in pyruvate dehydrogenase, acyl-CoA dehydrogenase, cytochrome c oxidase, and ATP synthase and a decrease in acetyl-CoA carboxylase (from day 3) were observed. Similar to adiponectin, protein level of resistin showed a biphasic profile: it increased after days 1, 3, and 7 and decreased below the control after 21 days of cold-acclimation.
In summary, the data suggest that adiponectin and resistin are important integrators of RpWAT metabolic response and roles it plays during cold acclimation. It seems that AMPKα mediate adiponectin effects on metabolic remodeling RpWAT during cold acclimation. |
| format | Article |
| id | doaj-art-de0f7d081207400ba2f440f78d9a2ace |
| institution | Kabale University |
| issn | 2090-0708 2090-0716 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Obesity |
| spelling | doaj-art-de0f7d081207400ba2f440f78d9a2ace2025-02-03T05:58:33ZengWileyJournal of Obesity2090-07082090-07162013-01-01201310.1155/2013/937572937572Endocrine and Metabolic Signaling in Retroperitoneal White Adipose Tissue Remodeling during Cold AcclimationAleksandra Jankovic0Aleksandra Korac1Biljana Buzadzic2Vesna Otasevic3Ana Stancic4Milica Vucetic5Milica Markelic6Ksenija Velickovic7Igor Golic8Bato Korac9Department of Physiology, Institute for Biological Research “Sinisa Stankovic”, University of Belgrade, 11060 Belgrade, SerbiaFaculty of Biology, Center for Electron Microscopy, University of Belgrade, 11000 Belgrade, SerbiaDepartment of Physiology, Institute for Biological Research “Sinisa Stankovic”, University of Belgrade, 11060 Belgrade, SerbiaDepartment of Physiology, Institute for Biological Research “Sinisa Stankovic”, University of Belgrade, 11060 Belgrade, SerbiaDepartment of Physiology, Institute for Biological Research “Sinisa Stankovic”, University of Belgrade, 11060 Belgrade, SerbiaDepartment of Physiology, Institute for Biological Research “Sinisa Stankovic”, University of Belgrade, 11060 Belgrade, SerbiaFaculty of Biology, Center for Electron Microscopy, University of Belgrade, 11000 Belgrade, SerbiaFaculty of Biology, Center for Electron Microscopy, University of Belgrade, 11000 Belgrade, SerbiaFaculty of Biology, Center for Electron Microscopy, University of Belgrade, 11000 Belgrade, SerbiaDepartment of Physiology, Institute for Biological Research “Sinisa Stankovic”, University of Belgrade, 11060 Belgrade, SerbiaThe expression profiles of adiponectin, resistin, 5′-AMP-activated protein kinase α (AMPKα), hypoxia-inducible factor-1α (HIF-1α), and key enzymes of glucose and fatty acid metabolism and oxidative phosphorylation in rat retroperitoneal white adipose tissue (RpWAT) during 45-day cold acclimation were examined. After transient suppression on day 1, adiponectin protein level increased following sustained cold exposure. In parallel, on day 1, the protein level of HIF-1α was strongly induced and AMPKα suppressed, while afterwards the reverse was seen. What is more, after an initial decrease on day 1, a sequential increase in pyruvate dehydrogenase, acyl-CoA dehydrogenase, cytochrome c oxidase, and ATP synthase and a decrease in acetyl-CoA carboxylase (from day 3) were observed. Similar to adiponectin, protein level of resistin showed a biphasic profile: it increased after days 1, 3, and 7 and decreased below the control after 21 days of cold-acclimation. In summary, the data suggest that adiponectin and resistin are important integrators of RpWAT metabolic response and roles it plays during cold acclimation. It seems that AMPKα mediate adiponectin effects on metabolic remodeling RpWAT during cold acclimation.http://dx.doi.org/10.1155/2013/937572 |
| spellingShingle | Aleksandra Jankovic Aleksandra Korac Biljana Buzadzic Vesna Otasevic Ana Stancic Milica Vucetic Milica Markelic Ksenija Velickovic Igor Golic Bato Korac Endocrine and Metabolic Signaling in Retroperitoneal White Adipose Tissue Remodeling during Cold Acclimation Journal of Obesity |
| title | Endocrine and Metabolic Signaling in Retroperitoneal White Adipose Tissue Remodeling during Cold Acclimation |
| title_full | Endocrine and Metabolic Signaling in Retroperitoneal White Adipose Tissue Remodeling during Cold Acclimation |
| title_fullStr | Endocrine and Metabolic Signaling in Retroperitoneal White Adipose Tissue Remodeling during Cold Acclimation |
| title_full_unstemmed | Endocrine and Metabolic Signaling in Retroperitoneal White Adipose Tissue Remodeling during Cold Acclimation |
| title_short | Endocrine and Metabolic Signaling in Retroperitoneal White Adipose Tissue Remodeling during Cold Acclimation |
| title_sort | endocrine and metabolic signaling in retroperitoneal white adipose tissue remodeling during cold acclimation |
| url | http://dx.doi.org/10.1155/2013/937572 |
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