Adventitious shoot growth in Grammatophylum scriptum var. Leopard Blume under colchicine and oryzalin applications

Grammatophylum scriptum var. Leopard Blume is of great demand due to its uniqueness flowers showing tiger-like patterns in color. This can still be developed further to make it the main element of a cut flower arrangement. Polyploidization offers the potentials of producing better plant vigour and p...

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Main Authors: Dwiati Murni, Hery Susanto Agus
Format: Article
Language:English
Published: EDP Sciences 2025-01-01
Series:BIO Web of Conferences
Online Access:https://www.bio-conferences.org/articles/bioconf/pdf/2025/09/bioconf_icsard2024_03014.pdf
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author Dwiati Murni
Hery Susanto Agus
author_facet Dwiati Murni
Hery Susanto Agus
author_sort Dwiati Murni
collection DOAJ
description Grammatophylum scriptum var. Leopard Blume is of great demand due to its uniqueness flowers showing tiger-like patterns in color. This can still be developed further to make it the main element of a cut flower arrangement. Polyploidization offers the potentials of producing better plant vigour and performance in comparison to its diploid counterparts. This study aims to investigate the effectiveness of colchicine and oryzalin in inducing polyploidization in G. scriptum var. Leopard and to determine the most optimum concentration of the substances in producing desired polyploid G. scriptum var. Leopard. An experiment method was arranged in a Completely Randomized Design employing eight treatments consisting of four colchicine concentrations and four oryzalin concentrations. Some morpho-anatomical characters were used as the parameters to examine. Plants under both colchicine and oryzalin showed fresh green in colour. Even sturdier and larger size were observed in the oryzalin-treated plants. Stomata and epidermal cells enlarged leading to a decrease in stomata number and density. It can be concluded that oryzalin of 75 μM was found to be the most optimum treatment as it produced plants of wide and sturdy leaves supported by numerous roots and higher plant habitus.
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spelling doaj-art-de0bca773e184c2aa5328513d5ff1ee72025-08-20T03:12:39ZengEDP SciencesBIO Web of Conferences2117-44582025-01-011580301410.1051/bioconf/202515803014bioconf_icsard2024_03014Adventitious shoot growth in Grammatophylum scriptum var. Leopard Blume under colchicine and oryzalin applicationsDwiati Murni0Hery Susanto Agus1The Faculty of Biology Universitas Jenderal SoedirmanThe Faculty of Biology Universitas Jenderal SoedirmanGrammatophylum scriptum var. Leopard Blume is of great demand due to its uniqueness flowers showing tiger-like patterns in color. This can still be developed further to make it the main element of a cut flower arrangement. Polyploidization offers the potentials of producing better plant vigour and performance in comparison to its diploid counterparts. This study aims to investigate the effectiveness of colchicine and oryzalin in inducing polyploidization in G. scriptum var. Leopard and to determine the most optimum concentration of the substances in producing desired polyploid G. scriptum var. Leopard. An experiment method was arranged in a Completely Randomized Design employing eight treatments consisting of four colchicine concentrations and four oryzalin concentrations. Some morpho-anatomical characters were used as the parameters to examine. Plants under both colchicine and oryzalin showed fresh green in colour. Even sturdier and larger size were observed in the oryzalin-treated plants. Stomata and epidermal cells enlarged leading to a decrease in stomata number and density. It can be concluded that oryzalin of 75 μM was found to be the most optimum treatment as it produced plants of wide and sturdy leaves supported by numerous roots and higher plant habitus.https://www.bio-conferences.org/articles/bioconf/pdf/2025/09/bioconf_icsard2024_03014.pdf
spellingShingle Dwiati Murni
Hery Susanto Agus
Adventitious shoot growth in Grammatophylum scriptum var. Leopard Blume under colchicine and oryzalin applications
BIO Web of Conferences
title Adventitious shoot growth in Grammatophylum scriptum var. Leopard Blume under colchicine and oryzalin applications
title_full Adventitious shoot growth in Grammatophylum scriptum var. Leopard Blume under colchicine and oryzalin applications
title_fullStr Adventitious shoot growth in Grammatophylum scriptum var. Leopard Blume under colchicine and oryzalin applications
title_full_unstemmed Adventitious shoot growth in Grammatophylum scriptum var. Leopard Blume under colchicine and oryzalin applications
title_short Adventitious shoot growth in Grammatophylum scriptum var. Leopard Blume under colchicine and oryzalin applications
title_sort adventitious shoot growth in grammatophylum scriptum var leopard blume under colchicine and oryzalin applications
url https://www.bio-conferences.org/articles/bioconf/pdf/2025/09/bioconf_icsard2024_03014.pdf
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