«FREE» AND «BOUND» ANTIBODIES AGAINST HIV–1 STRUCTURE PROTEINS AT EARLY STAGE OF THE DISEASE
Abstract. Using ELISA and immunoblotting we have monitored the activity of “free” antibodies against virus structure proteins in the sera of HIV–infected patients at the stage of the developing humoral immune response. Further, a comparative assessment of “free” and “bound” antibodies frequencies in...
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| Format: | Article |
| Language: | Russian |
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St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists
2014-07-01
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| Series: | Медицинская иммунология |
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| Online Access: | https://www.mimmun.ru/mimmun/article/view/430 |
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| author | G. A. Rjazanova V. P. Kocksin R. V. Khamzina |
| author_facet | G. A. Rjazanova V. P. Kocksin R. V. Khamzina |
| author_sort | G. A. Rjazanova |
| collection | DOAJ |
| description | Abstract. Using ELISA and immunoblotting we have monitored the activity of “free” antibodies against virus structure proteins in the sera of HIV–infected patients at the stage of the developing humoral immune response. Further, a comparative assessment of “free” and “bound” antibodies frequencies in the sera of HIV–infection patients at early stage of the disease has been made. We have obtained two fractions of circulating immune complex (CIC1 and CIC2) using precipitation in polyethylenglycol.We have determined, that antibodies against virus proteins coded by gag, pot and env genes formed immune complex dissociating under +60°C.Using non parametric criterion for z–signs. We have shown significant differences between the frequency on antibodies against virus structure proteins in sera and the presence of these antibodies in immune complexes. (Med. Immunol., 2005, vol.7, № 1, pp. 73576) |
| format | Article |
| id | doaj-art-ddedc3adc5cb4f8c98b38b62bca5a61b |
| institution | Kabale University |
| issn | 1563-0625 2313-741X |
| language | Russian |
| publishDate | 2014-07-01 |
| publisher | St. Petersburg branch of the Russian Association of Allergologists and Clinical Immunologists |
| record_format | Article |
| series | Медицинская иммунология |
| spelling | doaj-art-ddedc3adc5cb4f8c98b38b62bca5a61b2025-08-20T04:00:09ZrusSt. Petersburg branch of the Russian Association of Allergologists and Clinical ImmunologistsМедицинская иммунология1563-06252313-741X2014-07-0171737610.15789/1563-0625-2005-1-73-76427«FREE» AND «BOUND» ANTIBODIES AGAINST HIV–1 STRUCTURE PROTEINS AT EARLY STAGE OF THE DISEASEG. A. Rjazanova0V. P. Kocksin1R. V. Khamzina2Республиканский центр по профилактике и борьбе со СПИД и инфекционными заболеваниями МЗ РТ, КазаньРеспубликанский центр по профилактике и борьбе со СПИД и инфекционными заболеваниями МЗ РТ, КазаньРеспубликанский центр по профилактике и борьбе со СПИД и инфекционными заболеваниями МЗ РТ, КазаньAbstract. Using ELISA and immunoblotting we have monitored the activity of “free” antibodies against virus structure proteins in the sera of HIV–infected patients at the stage of the developing humoral immune response. Further, a comparative assessment of “free” and “bound” antibodies frequencies in the sera of HIV–infection patients at early stage of the disease has been made. We have obtained two fractions of circulating immune complex (CIC1 and CIC2) using precipitation in polyethylenglycol.We have determined, that antibodies against virus proteins coded by gag, pot and env genes formed immune complex dissociating under +60°C.Using non parametric criterion for z–signs. We have shown significant differences between the frequency on antibodies against virus structure proteins in sera and the presence of these antibodies in immune complexes. (Med. Immunol., 2005, vol.7, № 1, pp. 73576)https://www.mimmun.ru/mimmun/article/view/430hiv-1antibodiesimmune complex |
| spellingShingle | G. A. Rjazanova V. P. Kocksin R. V. Khamzina «FREE» AND «BOUND» ANTIBODIES AGAINST HIV–1 STRUCTURE PROTEINS AT EARLY STAGE OF THE DISEASE Медицинская иммунология hiv-1 antibodies immune complex |
| title | «FREE» AND «BOUND» ANTIBODIES AGAINST HIV–1 STRUCTURE PROTEINS AT EARLY STAGE OF THE DISEASE |
| title_full | «FREE» AND «BOUND» ANTIBODIES AGAINST HIV–1 STRUCTURE PROTEINS AT EARLY STAGE OF THE DISEASE |
| title_fullStr | «FREE» AND «BOUND» ANTIBODIES AGAINST HIV–1 STRUCTURE PROTEINS AT EARLY STAGE OF THE DISEASE |
| title_full_unstemmed | «FREE» AND «BOUND» ANTIBODIES AGAINST HIV–1 STRUCTURE PROTEINS AT EARLY STAGE OF THE DISEASE |
| title_short | «FREE» AND «BOUND» ANTIBODIES AGAINST HIV–1 STRUCTURE PROTEINS AT EARLY STAGE OF THE DISEASE |
| title_sort | free and bound antibodies against hiv 1 structure proteins at early stage of the disease |
| topic | hiv-1 antibodies immune complex |
| url | https://www.mimmun.ru/mimmun/article/view/430 |
| work_keys_str_mv | AT garjazanova freeandboundantibodiesagainsthiv1structureproteinsatearlystageofthedisease AT vpkocksin freeandboundantibodiesagainsthiv1structureproteinsatearlystageofthedisease AT rvkhamzina freeandboundantibodiesagainsthiv1structureproteinsatearlystageofthedisease |