Anti-Inflammatory, Antithrombotic, and Antioxidant Properties of Amphiphilic Lipid Bioactives from Shrimp
<b>Background/Objectives:</b> Marine organisms, including shrimps, have gained research interest due to containing an abundance of bioactive lipid molecules.This study evaluated the composition and the in vitro biological activities of amphiphilic bioactive compounds from four different...
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2024-12-01
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| author | Alexandros Tsoupras Paschalis Cholidis Dimitrios Kranas Evangelia Aikaterini Galouni Anna Ofrydopoulou Pavlos Efthymiopoulos Katie Shiels Sushanta Kumar Saha George Z. Kyzas Chryssa Anastasiadou |
| author_facet | Alexandros Tsoupras Paschalis Cholidis Dimitrios Kranas Evangelia Aikaterini Galouni Anna Ofrydopoulou Pavlos Efthymiopoulos Katie Shiels Sushanta Kumar Saha George Z. Kyzas Chryssa Anastasiadou |
| author_sort | Alexandros Tsoupras |
| collection | DOAJ |
| description | <b>Background/Objectives:</b> Marine organisms, including shrimps, have gained research interest due to containing an abundance of bioactive lipid molecules.This study evaluated the composition and the in vitro biological activities of amphiphilic bioactive compounds from four different wild shrimp species: <i>Litopenaeus vannamei</i>, <i>Penaeus kerathurus</i>, <i>Aristaeomorpha foliacea</i>, and <i>Parapenaeus longirostris</i>. <b>Methods:</b> Total lipid (TL) extracts were obtained from shrimp and separated into total amphiphilic (TAC) and total lipophilic (TLC) compounds. Phenolic (TPC) and carotenoid (TCC) contents, antioxidant activities (DPPH, ABTS, FRAP assays), and biological effects on platelet-activating factor (PAF) and ADP-induced platelet activation were evaluated. Structural analyses were performed using ATR-FTIR spectroscopy, while LC-MS was used to elucidate the fatty acid composition and overall structure of polar lipids (PLs) present in shrimp TAC extracts. <b>Results:</b> TAC extracts, rich in phenolics, carotenoids, PL, and unsaturated fatty acids (UFAs), exhibited stronger anti-inflammatory and antithrombotic activities compared with TLC extracts, which showed potent antioxidant capacity. Significant amounts of UFAs, such as the monounsaturated fatty acid (MUFA) oleic acid (C18:1n9) and omega-3 (n3) polyunsaturated fatty acids (PUFAs) like eicosapentaenoic acid (EPA; C20:5n3) and docosahexaenoic acid (DHA; C22:6n3), were detected in the PLs of shrimp TAC extracts, with favorable anti-inflammatory values for their n6/n3 PUFA ratio. Shrimp amphiphilic bioactives present in the TAC extracts provide anti-inflammatory effects against the PAF pathway and antithrombotic effects against ADP and eicosanoid pathways. <b>Conclusions</b>: The overall findings support further study on the use of shrimp extracts rich in anti-inflammatory, anti-thrombotic, and antioxidant amphiphilic bioactives as ingredients to produce new bio-functional health-promoting products, in the context of sustainable development and circular economy. |
| format | Article |
| id | doaj-art-dde8f1e75f354ae9b93d401336d3c81a |
| institution | Kabale University |
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| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-dde8f1e75f354ae9b93d401336d3c81a2025-01-24T13:45:03ZengMDPI AGPharmaceuticals1424-82472024-12-011812510.3390/ph18010025Anti-Inflammatory, Antithrombotic, and Antioxidant Properties of Amphiphilic Lipid Bioactives from ShrimpAlexandros Tsoupras0Paschalis Cholidis1Dimitrios Kranas2Evangelia Aikaterini Galouni3Anna Ofrydopoulou4Pavlos Efthymiopoulos5Katie Shiels6Sushanta Kumar Saha7George Z. Kyzas8Chryssa Anastasiadou9Hephaestus, Laboratory, School of Chemistry, Faculty of Sciences, Democritus University of Thrace, St Lukas, 65404 Kavala, GreeceHephaestus, Laboratory, School of Chemistry, Faculty of Sciences, Democritus University of Thrace, St Lukas, 65404 Kavala, GreeceHephaestus, Laboratory, School of Chemistry, Faculty of Sciences, Democritus University of Thrace, St Lukas, 65404 Kavala, GreeceHephaestus, Laboratory, School of Chemistry, Faculty of Sciences, Democritus University of Thrace, St Lukas, 65404 Kavala, GreeceHephaestus, Laboratory, School of Chemistry, Faculty of Sciences, Democritus University of Thrace, St Lukas, 65404 Kavala, GreeceHephaestus, Laboratory, School of Chemistry, Faculty of Sciences, Democritus University of Thrace, St Lukas, 65404 Kavala, GreeceCentre for Applied Bioscience Research, Technological University of the Shannon: Midlands Midwest, Moylish Park, V94 E8YF Limerick, IrelandCentre for Applied Bioscience Research, Technological University of the Shannon: Midlands Midwest, Moylish Park, V94 E8YF Limerick, IrelandHephaestus, Laboratory, School of Chemistry, Faculty of Sciences, Democritus University of Thrace, St Lukas, 65404 Kavala, GreeceFisheries Research Institute, Nea Peramos, 64007 Kavala, Greece<b>Background/Objectives:</b> Marine organisms, including shrimps, have gained research interest due to containing an abundance of bioactive lipid molecules.This study evaluated the composition and the in vitro biological activities of amphiphilic bioactive compounds from four different wild shrimp species: <i>Litopenaeus vannamei</i>, <i>Penaeus kerathurus</i>, <i>Aristaeomorpha foliacea</i>, and <i>Parapenaeus longirostris</i>. <b>Methods:</b> Total lipid (TL) extracts were obtained from shrimp and separated into total amphiphilic (TAC) and total lipophilic (TLC) compounds. Phenolic (TPC) and carotenoid (TCC) contents, antioxidant activities (DPPH, ABTS, FRAP assays), and biological effects on platelet-activating factor (PAF) and ADP-induced platelet activation were evaluated. Structural analyses were performed using ATR-FTIR spectroscopy, while LC-MS was used to elucidate the fatty acid composition and overall structure of polar lipids (PLs) present in shrimp TAC extracts. <b>Results:</b> TAC extracts, rich in phenolics, carotenoids, PL, and unsaturated fatty acids (UFAs), exhibited stronger anti-inflammatory and antithrombotic activities compared with TLC extracts, which showed potent antioxidant capacity. Significant amounts of UFAs, such as the monounsaturated fatty acid (MUFA) oleic acid (C18:1n9) and omega-3 (n3) polyunsaturated fatty acids (PUFAs) like eicosapentaenoic acid (EPA; C20:5n3) and docosahexaenoic acid (DHA; C22:6n3), were detected in the PLs of shrimp TAC extracts, with favorable anti-inflammatory values for their n6/n3 PUFA ratio. Shrimp amphiphilic bioactives present in the TAC extracts provide anti-inflammatory effects against the PAF pathway and antithrombotic effects against ADP and eicosanoid pathways. <b>Conclusions</b>: The overall findings support further study on the use of shrimp extracts rich in anti-inflammatory, anti-thrombotic, and antioxidant amphiphilic bioactives as ingredients to produce new bio-functional health-promoting products, in the context of sustainable development and circular economy.https://www.mdpi.com/1424-8247/18/1/25shrimpamphiphilic bioactivesmarine phenolicscarotenoidspolar lipidsUFA |
| spellingShingle | Alexandros Tsoupras Paschalis Cholidis Dimitrios Kranas Evangelia Aikaterini Galouni Anna Ofrydopoulou Pavlos Efthymiopoulos Katie Shiels Sushanta Kumar Saha George Z. Kyzas Chryssa Anastasiadou Anti-Inflammatory, Antithrombotic, and Antioxidant Properties of Amphiphilic Lipid Bioactives from Shrimp Pharmaceuticals shrimp amphiphilic bioactives marine phenolics carotenoids polar lipids UFA |
| title | Anti-Inflammatory, Antithrombotic, and Antioxidant Properties of Amphiphilic Lipid Bioactives from Shrimp |
| title_full | Anti-Inflammatory, Antithrombotic, and Antioxidant Properties of Amphiphilic Lipid Bioactives from Shrimp |
| title_fullStr | Anti-Inflammatory, Antithrombotic, and Antioxidant Properties of Amphiphilic Lipid Bioactives from Shrimp |
| title_full_unstemmed | Anti-Inflammatory, Antithrombotic, and Antioxidant Properties of Amphiphilic Lipid Bioactives from Shrimp |
| title_short | Anti-Inflammatory, Antithrombotic, and Antioxidant Properties of Amphiphilic Lipid Bioactives from Shrimp |
| title_sort | anti inflammatory antithrombotic and antioxidant properties of amphiphilic lipid bioactives from shrimp |
| topic | shrimp amphiphilic bioactives marine phenolics carotenoids polar lipids UFA |
| url | https://www.mdpi.com/1424-8247/18/1/25 |
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