Mechanism by Which Laser Irradiation Combined with Spirulina platensis Enhances FeSO4-Induced Ferroptosis in Salmonella Typhimurium and Listeria monocytogenes
To explore the mechanism by which laser (L) irradiation combined with Spirulina platensis (SP) enhances ferroptosis induced by FeSO4 (Fe) in Salmonella enterica serovar Typhimurium (ST) and Listeria monocytogenes (LM), this study measured lipid peroxidation, reactive oxygen species (ROS), glutathion...
Saved in:
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
China Food Publishing Company
2025-01-01
|
| Series: | Shipin Kexue |
| Subjects: | |
| Online Access: | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-2-002.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832539786421731328 |
|---|---|
| author | SUN Jinyue, PAN Jianeng, CHEN Yiying, LIU Rencheng, ZHOU Wenwen |
| author_facet | SUN Jinyue, PAN Jianeng, CHEN Yiying, LIU Rencheng, ZHOU Wenwen |
| author_sort | SUN Jinyue, PAN Jianeng, CHEN Yiying, LIU Rencheng, ZHOU Wenwen |
| collection | DOAJ |
| description | To explore the mechanism by which laser (L) irradiation combined with Spirulina platensis (SP) enhances ferroptosis induced by FeSO4 (Fe) in Salmonella enterica serovar Typhimurium (ST) and Listeria monocytogenes (LM), this study measured lipid peroxidation, reactive oxygen species (ROS), glutathione (GSH), morphological characteristics, cell membrane damage, and expression levels of key genes in the two foodborne pathogens after different treatments. The results showed that the L + SP treatment significantly enhanced the bactericidal effect of FeSO4. The 4 mmol/L Fe + SP + L treatment reduced the counts of both ST and LM to less than 1.4 (lg(CFU/mL)). Additionally, the Fe + SP + L treatment led to increased lipid peroxidation and ROS production and decreased proportion of GSH, thereby damaging the integrity of the cell membrane, increasing the permeability of the cell membrane, decreasing the membrane potential, increasing the leakage of intracellular nucleic acids and proteins, and ultimately resulting in the death of bacteria. In addition, the biofilm inhibition and inactivation experiments showed that the 4 mmol/L Fe + SP + L treatment had an excellent antibiofilm capacity. Finally, the counts of ST and LM on lettuce were significantly decreased to 4.63 and 4.60 (lg(CFU/g)), respectively, after the 4 mmol/L Fe + SP + L treatment. In conclusion, the Fe + SP + L treatment could effectively reduce the counts of ST and LM in both planktonic and biological states, indicating its good potential for controlling foodborne pathogens on the surface of lettuce and other foods. |
| format | Article |
| id | doaj-art-dddec5f92a1041c09475955ee4e800ba |
| institution | Kabale University |
| issn | 1002-6630 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | China Food Publishing Company |
| record_format | Article |
| series | Shipin Kexue |
| spelling | doaj-art-dddec5f92a1041c09475955ee4e800ba2025-02-05T09:08:22ZengChina Food Publishing CompanyShipin Kexue1002-66302025-01-0146281910.7506/spkx1002-6630-20240723-228Mechanism by Which Laser Irradiation Combined with Spirulina platensis Enhances FeSO4-Induced Ferroptosis in Salmonella Typhimurium and Listeria monocytogenesSUN Jinyue, PAN Jianeng, CHEN Yiying, LIU Rencheng, ZHOU Wenwen0(College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, China)To explore the mechanism by which laser (L) irradiation combined with Spirulina platensis (SP) enhances ferroptosis induced by FeSO4 (Fe) in Salmonella enterica serovar Typhimurium (ST) and Listeria monocytogenes (LM), this study measured lipid peroxidation, reactive oxygen species (ROS), glutathione (GSH), morphological characteristics, cell membrane damage, and expression levels of key genes in the two foodborne pathogens after different treatments. The results showed that the L + SP treatment significantly enhanced the bactericidal effect of FeSO4. The 4 mmol/L Fe + SP + L treatment reduced the counts of both ST and LM to less than 1.4 (lg(CFU/mL)). Additionally, the Fe + SP + L treatment led to increased lipid peroxidation and ROS production and decreased proportion of GSH, thereby damaging the integrity of the cell membrane, increasing the permeability of the cell membrane, decreasing the membrane potential, increasing the leakage of intracellular nucleic acids and proteins, and ultimately resulting in the death of bacteria. In addition, the biofilm inhibition and inactivation experiments showed that the 4 mmol/L Fe + SP + L treatment had an excellent antibiofilm capacity. Finally, the counts of ST and LM on lettuce were significantly decreased to 4.63 and 4.60 (lg(CFU/g)), respectively, after the 4 mmol/L Fe + SP + L treatment. In conclusion, the Fe + SP + L treatment could effectively reduce the counts of ST and LM in both planktonic and biological states, indicating its good potential for controlling foodborne pathogens on the surface of lettuce and other foods.https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-2-002.pdflaser; spirulina platensis; ferroptosis; lipid peroxidation; biofilm |
| spellingShingle | SUN Jinyue, PAN Jianeng, CHEN Yiying, LIU Rencheng, ZHOU Wenwen Mechanism by Which Laser Irradiation Combined with Spirulina platensis Enhances FeSO4-Induced Ferroptosis in Salmonella Typhimurium and Listeria monocytogenes Shipin Kexue laser; spirulina platensis; ferroptosis; lipid peroxidation; biofilm |
| title | Mechanism by Which Laser Irradiation Combined with Spirulina platensis Enhances FeSO4-Induced Ferroptosis in Salmonella Typhimurium and Listeria monocytogenes |
| title_full | Mechanism by Which Laser Irradiation Combined with Spirulina platensis Enhances FeSO4-Induced Ferroptosis in Salmonella Typhimurium and Listeria monocytogenes |
| title_fullStr | Mechanism by Which Laser Irradiation Combined with Spirulina platensis Enhances FeSO4-Induced Ferroptosis in Salmonella Typhimurium and Listeria monocytogenes |
| title_full_unstemmed | Mechanism by Which Laser Irradiation Combined with Spirulina platensis Enhances FeSO4-Induced Ferroptosis in Salmonella Typhimurium and Listeria monocytogenes |
| title_short | Mechanism by Which Laser Irradiation Combined with Spirulina platensis Enhances FeSO4-Induced Ferroptosis in Salmonella Typhimurium and Listeria monocytogenes |
| title_sort | mechanism by which laser irradiation combined with spirulina platensis enhances feso4 induced ferroptosis in salmonella typhimurium and listeria monocytogenes |
| topic | laser; spirulina platensis; ferroptosis; lipid peroxidation; biofilm |
| url | https://www.spkx.net.cn/fileup/1002-6630/PDF/2025-46-2-002.pdf |
| work_keys_str_mv | AT sunjinyuepanjianengchenyiyingliurenchengzhouwenwen mechanismbywhichlaserirradiationcombinedwithspirulinaplatensisenhancesfeso4inducedferroptosisinsalmonellatyphimuriumandlisteriamonocytogenes |