Actinidia deliciosa as a complemental therapy against nephropathy and oxidative stress in diabetic rats
This study aimed to evaluate the anti-hyperglycemic and antioxidant role of Actinidia deliciosa (kiwifruits) aqueous extract in streptozotocin-treated rats. Animals were distributed into; control, A. deliciosa aqueous extract (ADAE; 1 g/kg orally), streptozotocin (STZ; 50 mg/kg, i.p, single dose), a...
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Tsinghua University Press
2023-11-01
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| Series: | Food Science and Human Wellness |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221345302300071X |
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| author | Ali Y. Naoom Wenyi Kang Nora F. Ghanem Mohamed M. Abdel-Daim Fatma M. El-Demerdash |
| author_facet | Ali Y. Naoom Wenyi Kang Nora F. Ghanem Mohamed M. Abdel-Daim Fatma M. El-Demerdash |
| author_sort | Ali Y. Naoom |
| collection | DOAJ |
| description | This study aimed to evaluate the anti-hyperglycemic and antioxidant role of Actinidia deliciosa (kiwifruits) aqueous extract in streptozotocin-treated rats. Animals were distributed into; control, A. deliciosa aqueous extract (ADAE; 1 g/kg orally), streptozotocin (STZ; 50 mg/kg, i.p, single dose), and STZ plus ADAE, respectively. Results showed that ADAE had high antioxidant and radical scavenging potency. Elevation in blood sugar level, lipid peroxidation (LPO), kidney function biomarkers, and perturbations in hematological parameters were observed in diabetic rats. While, enzymatic and non-enzymatic antioxidants, protein content, and alkaline phosphatase (ALP) activity declined. Furthermore, histological, immunohistochemical alpha-smooth muscle actin immunoreactivity (α-SMA-ir) and histochemical (collagen, total protein, DNA, and RNA) alterations were observed in rat kidneys. Moreover, STZ produced upregulation of inflammatory associated genes (tumor necrosis factor-alpha; TNF-α and transforming growth factor β1; TGF-β1) and triggered apoptosis by upregulating apoptotic related gene [Bcl2-associated X protein (Bax)] and downregulating anti-apoptotic related gene B-cell lymphoma-2 (Bcl-2) based on real-time PCR data. Moreover, diabetic rats administered with ADAE showed significant restoration in LPO, antioxidant status, and biochemical indices besides tissue architecture, and genes improvement regarding STZ group. Conclusively, A. deliciosa has a valuable ameliorative influence and can restore glucose levels and improve kidney dysfunction in diabetic rats. |
| format | Article |
| id | doaj-art-ddc7ff7789e04b66a5a9dffdb59f81ad |
| institution | Kabale University |
| issn | 2213-4530 |
| language | English |
| publishDate | 2023-11-01 |
| publisher | Tsinghua University Press |
| record_format | Article |
| series | Food Science and Human Wellness |
| spelling | doaj-art-ddc7ff7789e04b66a5a9dffdb59f81ad2025-02-03T06:54:16ZengTsinghua University PressFood Science and Human Wellness2213-45302023-11-0112619811990Actinidia deliciosa as a complemental therapy against nephropathy and oxidative stress in diabetic ratsAli Y. Naoom0Wenyi Kang1Nora F. Ghanem2Mohamed M. Abdel-Daim3Fatma M. El-Demerdash4Department of Medical Laboratory Techniques, Imam Ja’afar Al-Sadiq University, Al-Muthanna 66002, IraqNational R & D Center for Edible Fungus Processing Technology, Henan University, Kaifeng 475004, ChinaDepartment of Zoology, Faculty of Science, Kafr ElSheikh University, Kafr El Sheikh 33516, EgyptDepartment of Pharmaceutical Sciences, Pharmacy Program, Batterjee Medical College, Jeddah 21442, Saudi Arabia; Pharmacology Department, Faculty of Veterinary Medicine, Suez Canal University, Ismailia 41522, EgyptDepartment of Environmental Studies, Institute of Graduate Studies and Research, Alexandria University, Alexandria 21526, Egypt; Corresponding author at: Department of Environmental Studies, Institute of Graduate Studies and Research, Alexandria University, Alexandria 21526, Egypt.This study aimed to evaluate the anti-hyperglycemic and antioxidant role of Actinidia deliciosa (kiwifruits) aqueous extract in streptozotocin-treated rats. Animals were distributed into; control, A. deliciosa aqueous extract (ADAE; 1 g/kg orally), streptozotocin (STZ; 50 mg/kg, i.p, single dose), and STZ plus ADAE, respectively. Results showed that ADAE had high antioxidant and radical scavenging potency. Elevation in blood sugar level, lipid peroxidation (LPO), kidney function biomarkers, and perturbations in hematological parameters were observed in diabetic rats. While, enzymatic and non-enzymatic antioxidants, protein content, and alkaline phosphatase (ALP) activity declined. Furthermore, histological, immunohistochemical alpha-smooth muscle actin immunoreactivity (α-SMA-ir) and histochemical (collagen, total protein, DNA, and RNA) alterations were observed in rat kidneys. Moreover, STZ produced upregulation of inflammatory associated genes (tumor necrosis factor-alpha; TNF-α and transforming growth factor β1; TGF-β1) and triggered apoptosis by upregulating apoptotic related gene [Bcl2-associated X protein (Bax)] and downregulating anti-apoptotic related gene B-cell lymphoma-2 (Bcl-2) based on real-time PCR data. Moreover, diabetic rats administered with ADAE showed significant restoration in LPO, antioxidant status, and biochemical indices besides tissue architecture, and genes improvement regarding STZ group. Conclusively, A. deliciosa has a valuable ameliorative influence and can restore glucose levels and improve kidney dysfunction in diabetic rats.http://www.sciencedirect.com/science/article/pii/S221345302300071XDiabetes mellitus;Actinidia deliciosaInflammationApoptosisOxidative stressNephropathy |
| spellingShingle | Ali Y. Naoom Wenyi Kang Nora F. Ghanem Mohamed M. Abdel-Daim Fatma M. El-Demerdash Actinidia deliciosa as a complemental therapy against nephropathy and oxidative stress in diabetic rats Food Science and Human Wellness Diabetes mellitus; Actinidia deliciosa Inflammation Apoptosis Oxidative stress Nephropathy |
| title | Actinidia deliciosa as a complemental therapy against nephropathy and oxidative stress in diabetic rats |
| title_full | Actinidia deliciosa as a complemental therapy against nephropathy and oxidative stress in diabetic rats |
| title_fullStr | Actinidia deliciosa as a complemental therapy against nephropathy and oxidative stress in diabetic rats |
| title_full_unstemmed | Actinidia deliciosa as a complemental therapy against nephropathy and oxidative stress in diabetic rats |
| title_short | Actinidia deliciosa as a complemental therapy against nephropathy and oxidative stress in diabetic rats |
| title_sort | actinidia deliciosa as a complemental therapy against nephropathy and oxidative stress in diabetic rats |
| topic | Diabetes mellitus; Actinidia deliciosa Inflammation Apoptosis Oxidative stress Nephropathy |
| url | http://www.sciencedirect.com/science/article/pii/S221345302300071X |
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