Determination of embryotoxic dose limit of detomidine with in-ovo model
<b>Aim:</b> The aim of this study is to determine the embryotoxic dose limit of detomidine with an in-ovo model using fertile hen?s eggs.<p> <b>Materials and Methods: </b>210 fertile eggs were divided into 7 equal (n: 30) groups and placed in the incubator under optimum...
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| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Selcuk University Press
2021-06-01
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| Series: | Eurasian Journal of Veterinary Sciences |
| Subjects: | |
| Online Access: | http://eurasianjvetsci.org/pdf.php3?id=1349 |
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| Summary: | <b>Aim:</b> The aim of this study is to determine the embryotoxic dose limit of
detomidine with an in-ovo model using fertile hen?s eggs.<p>
<b>Materials and Methods: </b>210 fertile eggs were divided into 7 equal (n:
30) groups and placed in the incubator under optimum conditions. No
application was made to the first group. On the seventh day, normal saline
solution, 10, 5, 2.5, 1.25, and 0.625 mg/kg doses of detomidine were administered
to egg air-sacs in the other groups, respectively. On the 21st day,
the eggs were opened and malformations, dead embryos and non-fertile
eggs were determined.<p>
<b>Results: </b>While 3, 2, 4, 6 and 5 dead embryos were determined in 0.625,
1.25, 2.5, 5, and 10 mg/kg treated groups, respectively, no dead embryos
were observed in the control and saline groups. While one non-fertile egg
was determined in the control and 5 mg/kg detomidine groups, no nonfertile
eggs were observed in the other groups. Mortality rates of detomidine
group at doses of 2.5, 5 and 10 mg/kg were higher than the control
and saline groups (p<0.05).<p>
<b>Conclusion:</b> Considering that the used smallest dose is 7.8 times higher
than the highest recommended dose for the target species, recommended
dose can be accepted safe in pregnant mammals for embryonic death. However,
considering that the dose should be 100 times lower as a safety
factor for mammals, it should be taken into account that negativities may
also be observed. |
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| ISSN: | 1309-6958 2146-1953 |