Mycobiome analyses of critically ill COVID-19 patients
ABSTRACT Coronavirus disease 2019 (COVID-19)-associated pulmonary aspergillosis (CAPA) is a life-threatening complication in patients with severe COVID-19. Previously, acute respiratory distress syndrome in patients with COVID-19 has been associated with lung fungal dysbiosis, evidenced by reduced m...
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| Language: | English |
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American Society for Microbiology
2025-02-01
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| Series: | Microbiology Spectrum |
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| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.04110-23 |
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| author | Danielle Weaver Sara Gago Matteo Bassetti Daniele Roberto Giacobbe Juergen Prattes Martin Hoenigl Florian Reizine Hélène Guegan Jean-Pierre Gangneux Michael John Bromley Paul Bowyer |
| author_facet | Danielle Weaver Sara Gago Matteo Bassetti Daniele Roberto Giacobbe Juergen Prattes Martin Hoenigl Florian Reizine Hélène Guegan Jean-Pierre Gangneux Michael John Bromley Paul Bowyer |
| author_sort | Danielle Weaver |
| collection | DOAJ |
| description | ABSTRACT Coronavirus disease 2019 (COVID-19)-associated pulmonary aspergillosis (CAPA) is a life-threatening complication in patients with severe COVID-19. Previously, acute respiratory distress syndrome in patients with COVID-19 has been associated with lung fungal dysbiosis, evidenced by reduced microbial diversity and Candida colonization. Increased fungal burden in the lungs of critically ill COVID-19 patients is linked to prolonged mechanical ventilation and increased mortality. However, specific mycobiome signatures associated with severe COVID-19 in the context of survival and antifungal drug prophylaxis have not yet been determined, and such knowledge could have an important impact on treatment. To understand the composition of the respiratory mycobiome in critically ill COVID-19 patients with and without CAPA and the impact of antifungal use in patient outcome, we performed a multinational study of 39 COVID-19 patients in intensive care units (ICUs). Respiratory mycobiome was profiled using internal transcribed spacer 1 sequencing, and Aspergillus fumigatus burden was further validated using quantitative PCR. Fungal communities were investigated using alpha diversity, beta diversity, taxa predominance, and taxa abundances. Respiratory mycobiomes of COVID-19 patients were dominated by Candida and Aspergillus. There was no significant association with corticosteroid use or CAPA diagnosis and respiratory fungal communities. Increased A. fumigatus burden was associated with mortality and, the use of azoles at ICU admission was linked with an absence of A. fumigatus. Our findings suggest that mold-active antifungal treatment at ICU admission may be linked with reduced A. fumigatus-associated mortality in severe COVID-19. However, further studies are warranted on this topic.IMPORTANCEInvasive fungal infections are a serious complication affecting up to a third of patients with severe COVID-19. Nevertheless, our understanding of the fungal communities in the lungs during critically ill COVID-19 remains limited. Evidence suggests a higher fungal burden is associated with prolonged ventilation and higher mortality, although the particular organisms responsible for this link are unclear. Antifungal prophylaxis may be beneficial for reducing the burden of fungal co-infections in COVID-19 intensive care. However, the composition of the fungal microbiome in severe COVID-19 in relation to prophylactic antifungals, as well as how this is associated with survival outcomes, is yet to be studied. Our study provides insights into the lung fungal microbiome in severe COVID-19 and has found antifungal treatment to be associated with lower Aspergillus fumigatus burden and that higher levels of this pathogen are associated with mortality. Therefore, our study suggests mold-active antifungal prophylaxis may be beneficial in severe COVID-19. |
| format | Article |
| id | doaj-art-ddaa6308e03c46cd84f578625326728c |
| institution | Kabale University |
| issn | 2165-0497 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj-art-ddaa6308e03c46cd84f578625326728c2025-02-04T14:03:40ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972025-02-0113210.1128/spectrum.04110-23Mycobiome analyses of critically ill COVID-19 patientsDanielle Weaver0Sara Gago1Matteo Bassetti2Daniele Roberto Giacobbe3Juergen Prattes4Martin Hoenigl5Florian Reizine6Hélène Guegan7Jean-Pierre Gangneux8Michael John Bromley9Paul Bowyer10University of Manchester, Manchester, United KingdomUniversity of Manchester, Manchester, United KingdomDepartment of Health Sciences (DISSAL), University of Genoa, Genoa, ItalyDepartment of Health Sciences (DISSAL), University of Genoa, Genoa, ItalyDivision of Infectious Diseases, Medical University of Graz, Graz, AustriaDivision of Infectious Diseases, Medical University of Graz, Graz, AustriaMedical Intensive Care Unit, Centre Hospitalier Universitaire de Rennes, Rennes, FranceCHU Rennes, Inserm, EHESP, Institut de Recherche en Santé, Environnement et Travail (IRSET), Université de Rennes, Rennes, FranceCHU Rennes, Inserm, EHESP, Institut de Recherche en Santé, Environnement et Travail (IRSET), Université de Rennes, Rennes, FranceUniversity of Manchester, Manchester, United KingdomUniversity of Manchester, Manchester, United KingdomABSTRACT Coronavirus disease 2019 (COVID-19)-associated pulmonary aspergillosis (CAPA) is a life-threatening complication in patients with severe COVID-19. Previously, acute respiratory distress syndrome in patients with COVID-19 has been associated with lung fungal dysbiosis, evidenced by reduced microbial diversity and Candida colonization. Increased fungal burden in the lungs of critically ill COVID-19 patients is linked to prolonged mechanical ventilation and increased mortality. However, specific mycobiome signatures associated with severe COVID-19 in the context of survival and antifungal drug prophylaxis have not yet been determined, and such knowledge could have an important impact on treatment. To understand the composition of the respiratory mycobiome in critically ill COVID-19 patients with and without CAPA and the impact of antifungal use in patient outcome, we performed a multinational study of 39 COVID-19 patients in intensive care units (ICUs). Respiratory mycobiome was profiled using internal transcribed spacer 1 sequencing, and Aspergillus fumigatus burden was further validated using quantitative PCR. Fungal communities were investigated using alpha diversity, beta diversity, taxa predominance, and taxa abundances. Respiratory mycobiomes of COVID-19 patients were dominated by Candida and Aspergillus. There was no significant association with corticosteroid use or CAPA diagnosis and respiratory fungal communities. Increased A. fumigatus burden was associated with mortality and, the use of azoles at ICU admission was linked with an absence of A. fumigatus. Our findings suggest that mold-active antifungal treatment at ICU admission may be linked with reduced A. fumigatus-associated mortality in severe COVID-19. However, further studies are warranted on this topic.IMPORTANCEInvasive fungal infections are a serious complication affecting up to a third of patients with severe COVID-19. Nevertheless, our understanding of the fungal communities in the lungs during critically ill COVID-19 remains limited. Evidence suggests a higher fungal burden is associated with prolonged ventilation and higher mortality, although the particular organisms responsible for this link are unclear. Antifungal prophylaxis may be beneficial for reducing the burden of fungal co-infections in COVID-19 intensive care. However, the composition of the fungal microbiome in severe COVID-19 in relation to prophylactic antifungals, as well as how this is associated with survival outcomes, is yet to be studied. Our study provides insights into the lung fungal microbiome in severe COVID-19 and has found antifungal treatment to be associated with lower Aspergillus fumigatus burden and that higher levels of this pathogen are associated with mortality. Therefore, our study suggests mold-active antifungal prophylaxis may be beneficial in severe COVID-19.https://journals.asm.org/doi/10.1128/spectrum.04110-23COVID-19CAPAmycobiomeAspergillus |
| spellingShingle | Danielle Weaver Sara Gago Matteo Bassetti Daniele Roberto Giacobbe Juergen Prattes Martin Hoenigl Florian Reizine Hélène Guegan Jean-Pierre Gangneux Michael John Bromley Paul Bowyer Mycobiome analyses of critically ill COVID-19 patients Microbiology Spectrum COVID-19 CAPA mycobiome Aspergillus |
| title | Mycobiome analyses of critically ill COVID-19 patients |
| title_full | Mycobiome analyses of critically ill COVID-19 patients |
| title_fullStr | Mycobiome analyses of critically ill COVID-19 patients |
| title_full_unstemmed | Mycobiome analyses of critically ill COVID-19 patients |
| title_short | Mycobiome analyses of critically ill COVID-19 patients |
| title_sort | mycobiome analyses of critically ill covid 19 patients |
| topic | COVID-19 CAPA mycobiome Aspergillus |
| url | https://journals.asm.org/doi/10.1128/spectrum.04110-23 |
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