Alteration of Loperamide-Induced Prostate Relaxation in High-Fat Diet-Fed Rats
Objective. To investigate the change of loperamide-induced prostate relaxation in rats fed with high-fat diet (HFD). Materials and Methods. Adult male Wistar rats were divided into 2 groups: (1) control rats fed with normal chow and (2) rats fed with HFD for 6 months. The prostate was removed for h...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/517836 |
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| _version_ | 1832567624481898496 |
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| author | Sheng-Lung Hsu Hsien-Hui Chung I-Hung Chen Yat-Ching Tong |
| author_facet | Sheng-Lung Hsu Hsien-Hui Chung I-Hung Chen Yat-Ching Tong |
| author_sort | Sheng-Lung Hsu |
| collection | DOAJ |
| description | Objective. To investigate the change of loperamide-induced prostate relaxation in rats fed with high-fat diet (HFD).
Materials and Methods. Adult male Wistar rats were divided into 2 groups: (1) control rats fed with normal chow and (2) rats fed with HFD for 6 months. The prostate was removed for histology study. Isolated prostate strips were hung in organ bath and precontracted with 1 μmol/L phenylephrine or 50 mmol/L KCl. The relaxation responses to loperamide 0.1 to 10 μmol/L were recorded. Western blotting analyses were performed for prostate μ-opioid receptors (MOR) and ATP-sensitive potassium (KATP) channel proteins: sulfonylurea receptor (SUR) and inwardly rectifying potassium channel (Kir) 6.2 subunits. Results. Body weight, prostate weight, plasma levels of glucose, insulin, triglyceride, and cholesterol, as well as systolic blood pressure, were significantly increased in the HFD rats. Histology showed prostatic hyperplasia in the HFD rat prostate. Prostatic relaxation induced by loperamide was markedly reduced in HFD when compared to the control. Protein expressions of MOR, SUR, and Kir 6.2 were decreased in HFD-fed rats. Conclusion. Loperamide-induced prostate relaxation is decreased in HFD rats due to reduced MOR and KATP channel expressions. |
| format | Article |
| id | doaj-art-dda5c66f2ce8457ba1ad53f0b42ce64f |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-dda5c66f2ce8457ba1ad53f0b42ce64f2025-02-03T01:01:00ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/517836517836Alteration of Loperamide-Induced Prostate Relaxation in High-Fat Diet-Fed RatsSheng-Lung Hsu0Hsien-Hui Chung1I-Hung Chen2Yat-Ching Tong3Department of Urology, Chi-Mei Medical Center Jiali, Jiali Town, Tainan City, TaiwanInstitute of Basic Medical Sciences, College of Medicine, National Cheng Kung University, Tainan City, TaiwanInstitute of Clinical Medical Sciences, College of Medicine, National Cheng Kung University, Tainan City, TaiwanDepartment of Urology, Medical College and Hospital, National Cheng Kung University, Tainan City, TaiwanObjective. To investigate the change of loperamide-induced prostate relaxation in rats fed with high-fat diet (HFD). Materials and Methods. Adult male Wistar rats were divided into 2 groups: (1) control rats fed with normal chow and (2) rats fed with HFD for 6 months. The prostate was removed for histology study. Isolated prostate strips were hung in organ bath and precontracted with 1 μmol/L phenylephrine or 50 mmol/L KCl. The relaxation responses to loperamide 0.1 to 10 μmol/L were recorded. Western blotting analyses were performed for prostate μ-opioid receptors (MOR) and ATP-sensitive potassium (KATP) channel proteins: sulfonylurea receptor (SUR) and inwardly rectifying potassium channel (Kir) 6.2 subunits. Results. Body weight, prostate weight, plasma levels of glucose, insulin, triglyceride, and cholesterol, as well as systolic blood pressure, were significantly increased in the HFD rats. Histology showed prostatic hyperplasia in the HFD rat prostate. Prostatic relaxation induced by loperamide was markedly reduced in HFD when compared to the control. Protein expressions of MOR, SUR, and Kir 6.2 were decreased in HFD-fed rats. Conclusion. Loperamide-induced prostate relaxation is decreased in HFD rats due to reduced MOR and KATP channel expressions.http://dx.doi.org/10.1155/2014/517836 |
| spellingShingle | Sheng-Lung Hsu Hsien-Hui Chung I-Hung Chen Yat-Ching Tong Alteration of Loperamide-Induced Prostate Relaxation in High-Fat Diet-Fed Rats The Scientific World Journal |
| title | Alteration of Loperamide-Induced Prostate Relaxation in High-Fat Diet-Fed Rats |
| title_full | Alteration of Loperamide-Induced Prostate Relaxation in High-Fat Diet-Fed Rats |
| title_fullStr | Alteration of Loperamide-Induced Prostate Relaxation in High-Fat Diet-Fed Rats |
| title_full_unstemmed | Alteration of Loperamide-Induced Prostate Relaxation in High-Fat Diet-Fed Rats |
| title_short | Alteration of Loperamide-Induced Prostate Relaxation in High-Fat Diet-Fed Rats |
| title_sort | alteration of loperamide induced prostate relaxation in high fat diet fed rats |
| url | http://dx.doi.org/10.1155/2014/517836 |
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