Exploring the Active Constituents of <i>Andrographis paniculata</i> in Protecting the Skin Barrier and the Synergistic Effects with Collagen XVII
<i>Andrographis paniculata</i> is mainly used to treat skin inflammations, wounds, and infections. In this study, Andrographis Herba, the aerial part of the plant, was proven to increase the viability of UVB-damaged HaCat cells and reduce reactive oxygen species levels. The chemical comp...
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| Format: | Article |
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MDPI AG
2025-01-01
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| Series: | Antioxidants |
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| Online Access: | https://www.mdpi.com/2076-3921/14/1/118 |
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| author | Heng Xu Shiying Lan Simin Lin Anjing Wang Yuanlin Luo Jing Wang Zhenzhong Yang |
| author_facet | Heng Xu Shiying Lan Simin Lin Anjing Wang Yuanlin Luo Jing Wang Zhenzhong Yang |
| author_sort | Heng Xu |
| collection | DOAJ |
| description | <i>Andrographis paniculata</i> is mainly used to treat skin inflammations, wounds, and infections. In this study, Andrographis Herba, the aerial part of the plant, was proven to increase the viability of UVB-damaged HaCat cells and reduce reactive oxygen species levels. The chemical composition of Andrographis Herba extract (AHE) was analyzed using UPLC-Q-TOF-MS, and diterpene lactones were identified as its primary constituents. Then, the fraction of diterpene lactones was prepared and exhibited similar effects to AHE. AHE, its diterpene lactones component, and its representative constituent andrographolide all decreased the expression of IL-1β, IL-6, and CDKN1A. Furthermore, the protective effects of AHE and its active ingredients on UVB-damaged epidermal stem cells were investigated. Notably, the combined treatment with andrographolide and collagen XVII enhanced the viability of UVB-damaged epidermal stem cells, increased the expression of stemness markers integrin β1 and p63, and decreased the expression of the differentiation marker keratin 10. This combination demonstrated significant synergy in maintaining skin homeostasis, which provides evidences for the development of skin-protective products. |
| format | Article |
| id | doaj-art-dd9a3066eb9443389b3c29396379487b |
| institution | Kabale University |
| issn | 2076-3921 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antioxidants |
| spelling | doaj-art-dd9a3066eb9443389b3c29396379487b2025-01-24T13:19:32ZengMDPI AGAntioxidants2076-39212025-01-0114111810.3390/antiox14010118Exploring the Active Constituents of <i>Andrographis paniculata</i> in Protecting the Skin Barrier and the Synergistic Effects with Collagen XVIIHeng Xu0Shiying Lan1Simin Lin2Anjing Wang3Yuanlin Luo4Jing Wang5Zhenzhong Yang6Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, ChinaPharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, ChinaProya Cosmetics Co., Ltd., Hangzhou 310023, ChinaPharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, ChinaPharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, ChinaProya Cosmetics Co., Ltd., Hangzhou 310023, ChinaPharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China<i>Andrographis paniculata</i> is mainly used to treat skin inflammations, wounds, and infections. In this study, Andrographis Herba, the aerial part of the plant, was proven to increase the viability of UVB-damaged HaCat cells and reduce reactive oxygen species levels. The chemical composition of Andrographis Herba extract (AHE) was analyzed using UPLC-Q-TOF-MS, and diterpene lactones were identified as its primary constituents. Then, the fraction of diterpene lactones was prepared and exhibited similar effects to AHE. AHE, its diterpene lactones component, and its representative constituent andrographolide all decreased the expression of IL-1β, IL-6, and CDKN1A. Furthermore, the protective effects of AHE and its active ingredients on UVB-damaged epidermal stem cells were investigated. Notably, the combined treatment with andrographolide and collagen XVII enhanced the viability of UVB-damaged epidermal stem cells, increased the expression of stemness markers integrin β1 and p63, and decreased the expression of the differentiation marker keratin 10. This combination demonstrated significant synergy in maintaining skin homeostasis, which provides evidences for the development of skin-protective products.https://www.mdpi.com/2076-3921/14/1/118skin homeostasisAndrographis Herbaditerpene lactonesandrographolidecollagen XVII |
| spellingShingle | Heng Xu Shiying Lan Simin Lin Anjing Wang Yuanlin Luo Jing Wang Zhenzhong Yang Exploring the Active Constituents of <i>Andrographis paniculata</i> in Protecting the Skin Barrier and the Synergistic Effects with Collagen XVII Antioxidants skin homeostasis Andrographis Herba diterpene lactones andrographolide collagen XVII |
| title | Exploring the Active Constituents of <i>Andrographis paniculata</i> in Protecting the Skin Barrier and the Synergistic Effects with Collagen XVII |
| title_full | Exploring the Active Constituents of <i>Andrographis paniculata</i> in Protecting the Skin Barrier and the Synergistic Effects with Collagen XVII |
| title_fullStr | Exploring the Active Constituents of <i>Andrographis paniculata</i> in Protecting the Skin Barrier and the Synergistic Effects with Collagen XVII |
| title_full_unstemmed | Exploring the Active Constituents of <i>Andrographis paniculata</i> in Protecting the Skin Barrier and the Synergistic Effects with Collagen XVII |
| title_short | Exploring the Active Constituents of <i>Andrographis paniculata</i> in Protecting the Skin Barrier and the Synergistic Effects with Collagen XVII |
| title_sort | exploring the active constituents of i andrographis paniculata i in protecting the skin barrier and the synergistic effects with collagen xvii |
| topic | skin homeostasis Andrographis Herba diterpene lactones andrographolide collagen XVII |
| url | https://www.mdpi.com/2076-3921/14/1/118 |
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