Identification of the Key Genes and Pathways in Esophageal Carcinoma
Objective. Esophageal carcinoma (EC) is a frequently common malignancy of gastrointestinal cancer in the world. This study aims to screen key genes and pathways in EC and elucidate the mechanism of it. Methods. 5 microarray datasets of EC were downloaded from Gene Expression Omnibus. Differentially...
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| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2016/2968106 |
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| author | Peng Su Shiwang Wen Yuefeng Zhang Yong Li Yanzhao Xu Yonggang Zhu Huilai Lv Fan Zhang Mingbo Wang Ziqiang Tian |
| author_facet | Peng Su Shiwang Wen Yuefeng Zhang Yong Li Yanzhao Xu Yonggang Zhu Huilai Lv Fan Zhang Mingbo Wang Ziqiang Tian |
| author_sort | Peng Su |
| collection | DOAJ |
| description | Objective. Esophageal carcinoma (EC) is a frequently common malignancy of gastrointestinal cancer in the world. This study aims to screen key genes and pathways in EC and elucidate the mechanism of it. Methods. 5 microarray datasets of EC were downloaded from Gene Expression Omnibus. Differentially expressed genes (DEGs) were screened by bioinformatics analysis. Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment, and protein-protein interaction (PPI) network construction were performed to obtain the biological roles of DEGs in EC. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to verify the expression level of DEGs in EC. Results. A total of 1955 genes were filtered as DEGs in EC. The upregulated genes were significantly enriched in cell cycle and the downregulated genes significantly enriched in Endocytosis. PPI network displayed CDK4 and CCT3 were hub proteins in the network. The expression level of 8 dysregulated DEGs including CDK4, CCT3, THSD4, SIM2, MYBL2, CENPF, CDCA3, and CDKN3 was validated in EC compared to adjacent nontumor tissues and the results were matched with the microarray analysis. Conclusion. The significantly DEGs including CDK4, CCT3, THSD4, and SIM2 may play key roles in tumorigenesis and development of EC involved in cell cycle and Endocytosis. |
| format | Article |
| id | doaj-art-dd708d0990b242b3a352190c4a9d2461 |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-dd708d0990b242b3a352190c4a9d24612025-02-03T01:10:41ZengWileyGastroenterology Research and Practice1687-61211687-630X2016-01-01201610.1155/2016/29681062968106Identification of the Key Genes and Pathways in Esophageal CarcinomaPeng Su0Shiwang Wen1Yuefeng Zhang2Yong Li3Yanzhao Xu4Yonggang Zhu5Huilai Lv6Fan Zhang7Mingbo Wang8Ziqiang Tian9Department of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, ChinaDepartment of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, ChinaDepartment of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, ChinaDepartment of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, ChinaDepartment of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, ChinaDepartment of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, ChinaDepartment of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, ChinaDepartment of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, ChinaDepartment of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, ChinaDepartment of Thoracic Surgery, The Fourth Hospital of Hebei Medical University, Shijiazhuang 050011, ChinaObjective. Esophageal carcinoma (EC) is a frequently common malignancy of gastrointestinal cancer in the world. This study aims to screen key genes and pathways in EC and elucidate the mechanism of it. Methods. 5 microarray datasets of EC were downloaded from Gene Expression Omnibus. Differentially expressed genes (DEGs) were screened by bioinformatics analysis. Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment, and protein-protein interaction (PPI) network construction were performed to obtain the biological roles of DEGs in EC. Quantitative real-time polymerase chain reaction (qRT-PCR) was used to verify the expression level of DEGs in EC. Results. A total of 1955 genes were filtered as DEGs in EC. The upregulated genes were significantly enriched in cell cycle and the downregulated genes significantly enriched in Endocytosis. PPI network displayed CDK4 and CCT3 were hub proteins in the network. The expression level of 8 dysregulated DEGs including CDK4, CCT3, THSD4, SIM2, MYBL2, CENPF, CDCA3, and CDKN3 was validated in EC compared to adjacent nontumor tissues and the results were matched with the microarray analysis. Conclusion. The significantly DEGs including CDK4, CCT3, THSD4, and SIM2 may play key roles in tumorigenesis and development of EC involved in cell cycle and Endocytosis.http://dx.doi.org/10.1155/2016/2968106 |
| spellingShingle | Peng Su Shiwang Wen Yuefeng Zhang Yong Li Yanzhao Xu Yonggang Zhu Huilai Lv Fan Zhang Mingbo Wang Ziqiang Tian Identification of the Key Genes and Pathways in Esophageal Carcinoma Gastroenterology Research and Practice |
| title | Identification of the Key Genes and Pathways in Esophageal Carcinoma |
| title_full | Identification of the Key Genes and Pathways in Esophageal Carcinoma |
| title_fullStr | Identification of the Key Genes and Pathways in Esophageal Carcinoma |
| title_full_unstemmed | Identification of the Key Genes and Pathways in Esophageal Carcinoma |
| title_short | Identification of the Key Genes and Pathways in Esophageal Carcinoma |
| title_sort | identification of the key genes and pathways in esophageal carcinoma |
| url | http://dx.doi.org/10.1155/2016/2968106 |
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