Investigating the Mutations in atpE and Rv0678 Genes in Mycobacterium Tuberculosis Clinical Isolates
Introduction: Tuberculosis (TB) caused by the bacterium Mycobacterium tuberculosis remains a critical global public health concern due to the high morbidity and mortality rates. Mutation in atpE and Rv0678 genes contributes to drug resistance in M. tuberculosis. This study investigates the antib...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Pasteur Institute of Iran
2023-12-01
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| Series: | Journal of Medical Microbiology and Infectious Diseases |
| Subjects: | |
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| Summary: | Introduction: Tuberculosis (TB) caused by the bacterium Mycobacterium
tuberculosis remains a critical global public health concern due to the high
morbidity and mortality rates. Mutation in atpE and Rv0678 genes
contributes to drug resistance in M. tuberculosis. This study investigates the
antibiotic resistance patterns and mutations in atpE and Rv0678 genes in 22
M. tuberculosis clinical isolates. Methods: Drug susceptibility testing (DST)
for rifampin, isoniazid, streptomycin, capreomycin, ofloxacin, kanamycin,
and ethambutol was conducted using the proportional method. This was
followed by determining the minimum inhibitory concentration (MIC) for
bedaquiline (BDQ) via the microplate Alamar blue assay (MABA). Genomic
regions encompassing atpE and Rv0678 genes were amplified and
sequenced for mutation analysis. Data analysis was performed using SPSS
software to interpret mutation patterns concerning drug susceptibility
profiles. Results: Of 22 isolates, 5 (27.8%) were extensively drug-resistant
tuberculosis (XDR-TB), and 13 (72.2%) were multi-drug resistant
tuberculosis (MDR-TB). Resistance rates to kanamycin, ofloxacin,
capreomycin, and streptomycin were 40.6%, 46.3%, 85%, and 74.6%,
respectively. Additionally, phenotypic resistance to bedaquiline was
observed in 12 (54.5%) isolates. Sequencing revealed no resistanceconferring
mutations in the atpE or Rv0678 genes among the isolates.
Conclusion: Our findings showed substantial resistance to first- and secondline
drugs in M. tuberculosis clinical isolates. This highlights the necessity
for ongoing, comprehensive studies to elucidate the evolving drug resistance
patterns and understand the underlying mechanisms in clinical isolates. |
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| ISSN: | 2345-5349 2345-5330 |