Analysis of the c.1135G > A, c.1993A > G, c.2059T > C TAP2 gene variants and their relationship with latent tuberculosis infection in Mexico

Tuberculosis (TB) is a worldwide public health problem with 10.6 million people falling ill and 1.5 million deaths every year. Latent tuberculosis infection (LTBI) is a condition in which an individual has been infected with Mycobacterium tuberculosis (Mtb) but does not show clinical signs and sympt...

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Main Authors: Gerardo Cazarez-Navarro, Ivan Hernández-Cañaveral, Ana Gabriela Colima-Fausto, Jaime Palomares-Marín, Karel Licona-Lasteros, Ana Laura Pereira-Suarez, Sergio Yair Rodríguez-Preciado
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Language:English
Published: Elsevier 2024-12-01
Series:Journal of Clinical Tuberculosis and Other Mycobacterial Diseases
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Online Access:http://www.sciencedirect.com/science/article/pii/S2405579424000883
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author Gerardo Cazarez-Navarro
Ivan Hernández-Cañaveral
Ana Gabriela Colima-Fausto
Jaime Palomares-Marín
Karel Licona-Lasteros
Ana Laura Pereira-Suarez
Sergio Yair Rodríguez-Preciado
author_facet Gerardo Cazarez-Navarro
Ivan Hernández-Cañaveral
Ana Gabriela Colima-Fausto
Jaime Palomares-Marín
Karel Licona-Lasteros
Ana Laura Pereira-Suarez
Sergio Yair Rodríguez-Preciado
author_sort Gerardo Cazarez-Navarro
collection DOAJ
description Tuberculosis (TB) is a worldwide public health problem with 10.6 million people falling ill and 1.5 million deaths every year. Latent tuberculosis infection (LTBI) is a condition in which an individual has been infected with Mycobacterium tuberculosis (Mtb) but does not show clinical signs and symptoms. The transporter associated with antigen processing (TAP2) protein plays a fundamental role in the immune response promoting the clearance of intracellular pathogens, such as Mtb. Our study aimed to determine the association between c.1135G > A (rs1800454), c.1993A > G (rs241447) and c.2059 T > C (rs241448) TAP2 gene variants with LTBI susceptibility. In this case-control study, 180 individuals (90 were LTBI-positive and 90 were controls) from shelters were analyzed. Genotyping of the polymorphisms was performed using the Applied Biosystems Step One Thermal Cycler Real-Time PCR allelic discrimination technology. The haplotypic analyses were performed with the Arlequin 3.5 software. The G allele (OR = 1.732, CI = 1.125–2.667, p = 0.012) and AG genotype of the c.1993A > G variant (p=<0.001) were associated with susceptibility to LTBI (p=<0.001), as well as the GAT, AAT, AAC, AGT haplotypes (p=<0.001). The c.1135G > A and c.2059 T > C variants were not associated with LTBI risk.
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spelling doaj-art-dd66d7e4fc2b4e2db95ee9e634477cc42025-08-20T01:58:56ZengElsevierJournal of Clinical Tuberculosis and Other Mycobacterial Diseases2405-57942024-12-013710050110.1016/j.jctube.2024.100501Analysis of the c.1135G > A, c.1993A > G, c.2059T > C TAP2 gene variants and their relationship with latent tuberculosis infection in MexicoGerardo Cazarez-Navarro0Ivan Hernández-Cañaveral1Ana Gabriela Colima-Fausto2Jaime Palomares-Marín3Karel Licona-Lasteros4Ana Laura Pereira-Suarez5Sergio Yair Rodríguez-Preciado6Secretaria de Salud Jalisco, Guadalajara, Jalisco, México, Dr. Baeza Alzaga 107, Zona Centro, Guadalajara 44100, Jalisco, MéxicoDepartamento de Microbiología y Patología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Independencia Oriente, Guadalajara 44340, Jalisco, MéxicoSchool of Medicine, Universidad Autónoma de Guadalajara, Universidad 700, Lomas del Valle, Guadalajara 45129, Jalisco, MéxicoDepartamento de Microbiología y Patología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Independencia Oriente, Guadalajara 44340, Jalisco, MéxicoDepartamento de Microbiología y Patología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Independencia Oriente, Guadalajara 44340, Jalisco, México; Laboratorio de Sistemas Biológicos, Departamento de Ciencias de la Salud, Centro Universitario de los Valles, Universidad de Guadalajara, Carretera Guadalajara - Ameca Km. 45.5, Ameca 46600, Jalisco, MéxicoDepartamento de Microbiología y Patología, Centro Universitario de Ciencias de la Salud, Universidad de Guadalajara, Sierra Mojada 950, Independencia Oriente, Guadalajara 44340, Jalisco, MéxicoLaboratorio de Sistemas Biológicos, Departamento de Ciencias de la Salud, Centro Universitario de los Valles, Universidad de Guadalajara, Carretera Guadalajara - Ameca Km. 45.5, Ameca 46600, Jalisco, México; Corresponding author at: Departamento de Ciencias de las Salud, Centro Universitario de los Valles, Universidad de Guadalajara, Carr. a Guadalajara Km. 45.5, CP 46600 Ameca, Jalisco, México.Tuberculosis (TB) is a worldwide public health problem with 10.6 million people falling ill and 1.5 million deaths every year. Latent tuberculosis infection (LTBI) is a condition in which an individual has been infected with Mycobacterium tuberculosis (Mtb) but does not show clinical signs and symptoms. The transporter associated with antigen processing (TAP2) protein plays a fundamental role in the immune response promoting the clearance of intracellular pathogens, such as Mtb. Our study aimed to determine the association between c.1135G > A (rs1800454), c.1993A > G (rs241447) and c.2059 T > C (rs241448) TAP2 gene variants with LTBI susceptibility. In this case-control study, 180 individuals (90 were LTBI-positive and 90 were controls) from shelters were analyzed. Genotyping of the polymorphisms was performed using the Applied Biosystems Step One Thermal Cycler Real-Time PCR allelic discrimination technology. The haplotypic analyses were performed with the Arlequin 3.5 software. The G allele (OR = 1.732, CI = 1.125–2.667, p = 0.012) and AG genotype of the c.1993A > G variant (p=<0.001) were associated with susceptibility to LTBI (p=<0.001), as well as the GAT, AAT, AAC, AGT haplotypes (p=<0.001). The c.1135G > A and c.2059 T > C variants were not associated with LTBI risk.http://www.sciencedirect.com/science/article/pii/S2405579424000883Latent tuberculosis infectionGenetic susceptibilityMycobacterium tuberculosisTAP2
spellingShingle Gerardo Cazarez-Navarro
Ivan Hernández-Cañaveral
Ana Gabriela Colima-Fausto
Jaime Palomares-Marín
Karel Licona-Lasteros
Ana Laura Pereira-Suarez
Sergio Yair Rodríguez-Preciado
Analysis of the c.1135G > A, c.1993A > G, c.2059T > C TAP2 gene variants and their relationship with latent tuberculosis infection in Mexico
Journal of Clinical Tuberculosis and Other Mycobacterial Diseases
Latent tuberculosis infection
Genetic susceptibility
Mycobacterium tuberculosis
TAP2
title Analysis of the c.1135G > A, c.1993A > G, c.2059T > C TAP2 gene variants and their relationship with latent tuberculosis infection in Mexico
title_full Analysis of the c.1135G > A, c.1993A > G, c.2059T > C TAP2 gene variants and their relationship with latent tuberculosis infection in Mexico
title_fullStr Analysis of the c.1135G > A, c.1993A > G, c.2059T > C TAP2 gene variants and their relationship with latent tuberculosis infection in Mexico
title_full_unstemmed Analysis of the c.1135G > A, c.1993A > G, c.2059T > C TAP2 gene variants and their relationship with latent tuberculosis infection in Mexico
title_short Analysis of the c.1135G > A, c.1993A > G, c.2059T > C TAP2 gene variants and their relationship with latent tuberculosis infection in Mexico
title_sort analysis of the c 1135g a c 1993a g c 2059t c tap2 gene variants and their relationship with latent tuberculosis infection in mexico
topic Latent tuberculosis infection
Genetic susceptibility
Mycobacterium tuberculosis
TAP2
url http://www.sciencedirect.com/science/article/pii/S2405579424000883
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