A Chemiluminescence Signal Amplification Method for MicroRNA Detection: The Combination of Molecular Aptamer Beacons with Enzyme-Free Hybridization Chain Reaction
The association between microRNA (miRNA) and various diseases has been established; miRNAs have the potential to be biomarkers for these diseases. Nevertheless, the challenge of correctly quantifying an miRNA arises from its low abundance and a high degree of family homology. Therefore, in the prese...
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MDPI AG
2024-12-01
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| Series: | Molecules |
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| Online Access: | https://www.mdpi.com/1420-3049/29/23/5782 |
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| author | Yu Han Jialin Li Man Li Ran An Xu Zhang Sheng Cai |
| author_facet | Yu Han Jialin Li Man Li Ran An Xu Zhang Sheng Cai |
| author_sort | Yu Han |
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| description | The association between microRNA (miRNA) and various diseases has been established; miRNAs have the potential to be biomarkers for these diseases. Nevertheless, the challenge of correctly quantifying an miRNA arises from its low abundance and a high degree of family homology. Therefore, in the present study, we devised a chemiluminescence (CL) detection method for miRNAs, known as the hybridization chain reaction (HCR)-CL, utilizing the enzyme-free signal amplification technology of HCR. The proposed methodology obviates the need for temperature conversion and offers a straightforward procedure owing to the absence of enzymatic participation, and the lumino-H<sub>2</sub>O<sub>2</sub>-mediated CL reaction occurs at a high rate. The technique successfully detected 2.5 amol of the target analyte and 50 amol of miR-146b in a 1% concentration of human serum. In summary, the method developed in this study is characterized by its ease of operation, cost-effectiveness, remarkable analytical prowess, and ability to detect miRNA without the need for total RNA extraction from serum samples. This method is expected to be widely used for biological sample testing in clinical settings. |
| format | Article |
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| institution | OA Journals |
| issn | 1420-3049 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
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| series | Molecules |
| spelling | doaj-art-dd603d3d9cd84d7882f7bd5304f99d2f2025-08-20T01:55:31ZengMDPI AGMolecules1420-30492024-12-012923578210.3390/molecules29235782A Chemiluminescence Signal Amplification Method for MicroRNA Detection: The Combination of Molecular Aptamer Beacons with Enzyme-Free Hybridization Chain ReactionYu Han0Jialin Li1Man Li2Ran An3Xu Zhang4Sheng Cai5College of Pharmacy, Jilin Medical University, Jilin 132013, ChinaCollege of Pharmacy, Jilin Medical University, Jilin 132013, ChinaCollege of Pharmacy, Jilin Medical University, Jilin 132013, ChinaCollege of Pharmacy, Jilin Medical University, Jilin 132013, ChinaCollege of Pharmacy, Jilin Medical University, Jilin 132013, ChinaInstitute of Drug Metabolism and Pharmaceutical Analysis, Zhejiang Province Key Laboratory of Anti-Cancer Drug Research, Zhejiang University, Hangzhou 310058, ChinaThe association between microRNA (miRNA) and various diseases has been established; miRNAs have the potential to be biomarkers for these diseases. Nevertheless, the challenge of correctly quantifying an miRNA arises from its low abundance and a high degree of family homology. Therefore, in the present study, we devised a chemiluminescence (CL) detection method for miRNAs, known as the hybridization chain reaction (HCR)-CL, utilizing the enzyme-free signal amplification technology of HCR. The proposed methodology obviates the need for temperature conversion and offers a straightforward procedure owing to the absence of enzymatic participation, and the lumino-H<sub>2</sub>O<sub>2</sub>-mediated CL reaction occurs at a high rate. The technique successfully detected 2.5 amol of the target analyte and 50 amol of miR-146b in a 1% concentration of human serum. In summary, the method developed in this study is characterized by its ease of operation, cost-effectiveness, remarkable analytical prowess, and ability to detect miRNA without the need for total RNA extraction from serum samples. This method is expected to be widely used for biological sample testing in clinical settings.https://www.mdpi.com/1420-3049/29/23/5782chemiluminescencehybridization chain reactionmiR-146bmolecular aptamer beacon |
| spellingShingle | Yu Han Jialin Li Man Li Ran An Xu Zhang Sheng Cai A Chemiluminescence Signal Amplification Method for MicroRNA Detection: The Combination of Molecular Aptamer Beacons with Enzyme-Free Hybridization Chain Reaction Molecules chemiluminescence hybridization chain reaction miR-146b molecular aptamer beacon |
| title | A Chemiluminescence Signal Amplification Method for MicroRNA Detection: The Combination of Molecular Aptamer Beacons with Enzyme-Free Hybridization Chain Reaction |
| title_full | A Chemiluminescence Signal Amplification Method for MicroRNA Detection: The Combination of Molecular Aptamer Beacons with Enzyme-Free Hybridization Chain Reaction |
| title_fullStr | A Chemiluminescence Signal Amplification Method for MicroRNA Detection: The Combination of Molecular Aptamer Beacons with Enzyme-Free Hybridization Chain Reaction |
| title_full_unstemmed | A Chemiluminescence Signal Amplification Method for MicroRNA Detection: The Combination of Molecular Aptamer Beacons with Enzyme-Free Hybridization Chain Reaction |
| title_short | A Chemiluminescence Signal Amplification Method for MicroRNA Detection: The Combination of Molecular Aptamer Beacons with Enzyme-Free Hybridization Chain Reaction |
| title_sort | chemiluminescence signal amplification method for microrna detection the combination of molecular aptamer beacons with enzyme free hybridization chain reaction |
| topic | chemiluminescence hybridization chain reaction miR-146b molecular aptamer beacon |
| url | https://www.mdpi.com/1420-3049/29/23/5782 |
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